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纸基微流体技术在即时检测中的应用 被引量:1
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作者 徐佳素 张雅 +2 位作者 苏晓崧 张师音 葛胜祥 《生物工程学报》 CAS CSCD 北大核心 2020年第7期1283-1292,共10页
即时检测(Point-of-care testing,POCT)是在采样现场或病人旁边进行的一种检测方式,它利用便携式分析仪器及配套试剂进行检测,能快速得到结果,被广泛应用于体外诊断行业。其中纸基微流体检测技术以其成本低廉、操作简单、检测快速、设... 即时检测(Point-of-care testing,POCT)是在采样现场或病人旁边进行的一种检测方式,它利用便携式分析仪器及配套试剂进行检测,能快速得到结果,被广泛应用于体外诊断行业。其中纸基微流体检测技术以其成本低廉、操作简单、检测快速、设备便携、应用不受场所限制等优点在发展现场POCT技术中具有非常大的潜力。近年来,纸基微流体技术的发展及其与各种新技术、新方法的融合推动了POCT技术和方法的实质性发展。文中简要概括了纸的分类与特性,介绍了基于纸基微流体技术发展而来的样本前处理方法、反应过程中的液流控制方式、反应后检测结果的读取与分析手段,综述了近年来在面向POCT应用中各类基于纸基微流体的分析装置(Microfluidicpaper-basedanalyticaldevices,μPADs)的研究进展,最后对纸基微流体技术在POCT应用中存在的问题进行了讨论,并对未来前景作了展望。 展开更多
关键词 纸基微流体 即时检测 体外诊断
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Influence of liquid water on coalbed methane adsorption:An experimental research on coal reservoirs in the south of Qinshui Basin 被引量:4
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作者 Shuxun Sang Yanming Zhu +2 位作者 Jing zhang Xiaodong zhang shiyin zhang 《Chinese Science Bulletin》 SCIE EI CAS 2005年第S1期79-85,共7页
Using Isothermal Adsorption/Desorption System Model IS-100 and Electrohydraulic Servo Rock System Model MTS815 as the main apparatuses and collecting samples from the major coal reservoirs in the south of Qinshui Basi... Using Isothermal Adsorption/Desorption System Model IS-100 and Electrohydraulic Servo Rock System Model MTS815 as the main apparatuses and collecting samples from the major coal reservoirs in the south of Qinshui Basin, a hot point region of coalbed methane exploration, the paper carries out systematical comparisons of the isothermal adsorption experimental data for injection water coal samples, equilibrium moisture samples and dry coal samples, probes and establishes an experimental method of injection water coal sample preparation and isothermal experiment to simulate real reservoir conditions, and then summaries the experimental regulations and discusses the mechanism of liquid water influencing coal methane adsorption. Results of the experiment indicate that: The Langmuir volume of injection water coal samples is notably larger than that of equilibrium moisture samples, as well as larger than or equivalent to that of dry coal samples; the Langmuir pressure of injection water coal samples is the highest, the next is equilibrium moisture samples, while the dry samples is the lowest, of which the experimental results of injection water samples to simulate real reservoir conditions are more close to the fact. Under the conditions of in-position reservoirs, liquid water in coals has evident influence on methane adsorption ability of coal matrix, which can increase the adsorbability of coal and make the adsorption regulation fit to Langmuir model better. Its major reason is the increase of wetting coal matrix adsorbability. The above experimental results overthrow the conventional cognition that liquid water has no influence on coalbed methane adsorption, which may lead to an improvement of the coalbed methane isothermal adsorption experimental method and of the reliability of coalbed methane resource evaluation and prediction. 展开更多
关键词 COAL RESERVOIR liquid water METHANE ADSORPTION INFLUENCE mechanism.
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Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
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作者 Tianli Zou Junhua Deng +8 位作者 Xiangdong Li shiyin zhang Lingyan Chen Liying Hao Jinshan Zhuang Heng Wang Guihong zhang Shengxiang Ge Kegong Tian 《Virologica Sinica》 SCIE CAS CSCD 2022年第3期462-464,共3页
Dear Editor,African swine fever virus(ASFV),the sole member of the family Asfarviridae,is the causative agent of African swine fever(ASF),a viral disease that leads to high mortality in domestic pigs.Since firstly ide... Dear Editor,African swine fever virus(ASFV),the sole member of the family Asfarviridae,is the causative agent of African swine fever(ASF),a viral disease that leads to high mortality in domestic pigs.Since firstly identified in Kenya in the 1920s,ASFV has been prevalent in Africa,Europe,and Russian Federation(Sanchez et al.,2019).Recently,ASFV was introduced to Asian countries including China,Mongolia,Vietnam,Cambodia,Laos and South Korea,which lead to huge economic losses to local pig industries(Li and Tian,2018;Gaudreault and Richt,2019).The first case of ASFV in China was reported in August 2018(Zhou et al.,2018). 展开更多
关键词 SWINE HYDROLYSIS African
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New discovery of high-affinity SARS-CoV-2 spike S2 protein binding peptide selected by PhIP-Seq
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作者 Mengyuan Chen Shuizhen He +17 位作者 Hualong Xiong Dongxu zhang Shaojuan Wang Wangheng Hou Xiaomei Zhu Jin Wang Yang Huang Congming Hong Yubin Wu Ruoyao Qi Tianying zhang Quan Yuan Tingdong Li Yixin Chen shiyin zhang Shengxiang Ge Jun zhang Ningshao Xia 《Virologica Sinica》 SCIE CAS CSCD 2022年第5期758-761,共4页
Dear Editor,Theworld had suffered pandemics of COVID-19 caused by SARS-CoV-2.At the time ofwriting,COVID-19 is still widely spreading inmany countries and regions,and by June 14,2022,totally 532,887,351 cases,includin... Dear Editor,Theworld had suffered pandemics of COVID-19 caused by SARS-CoV-2.At the time ofwriting,COVID-19 is still widely spreading inmany countries and regions,and by June 14,2022,totally 532,887,351 cases,including 6,307,021 deaths,were reported globally(WHO,https://covid19.who.int/table).Powerful measures,such as prophylactic and therapeutic interventions against the virus,are in urgent need for COVID-19 control and prevention.The spike protein,which is expressed on the virus surface,is the key determinant of viral-host interaction(Ng et al.,2021)and mediates the virus entry. 展开更多
关键词 PHIP totally
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