Efficient production of transgenic chickens using self-inactive HIV-based lentiviral vectors

We demonstrated the simple and effective production of transgenic chickens,in which the enhanced green fluorescence protein(EGFP)was expressed by using third-generation self-inactive HIV-based lentiviral vectors.In our experiments,lentiviruses were injected into 204 fertilized eggs,from which 30(15%)chickens were hatched.The exogenous gene was detected in the genomes of 16 out of 30(53%)chickens.The green fluorescence signal was observed directly in various body parts,and was particularly significant in the testes.The transgenes were also found in the offspring of these chickens.The results indicate that HIV-based lentiviral vectors can be used to generate transgenic birds economically and effectively.

Enhanced photocatalytic hydrogen production from Co-MOF/CN by nitrogen and sulfur co-doped coal-based carbon quantum dots

A novel composite photocatalyst for photocatalytic decomposition of water for hydrogen evolution was successfully synthesized by in-situ growth of nitrogen and sulfur co-doped coal-based carbon quantum dots(NSCQDs)nanoparticles on the surface of sheet cobalt-based metal-organic framework(Co-MOF)and graphitic carbon nitride(g-C_(3)N_(4),CN).The structure and properties of the obtained catalysts were systematically analyzed.NSCQDs effectively broaden the absorption of Co-MOF and CN in the visible region.The new composite photocatalyst has high hydrogen production activity and the hydrogen production rate reaches 6254μmol/(g·h)at pH=9.At the same time,NSCQDs synergy Co-MOF/CN composites have good stability.After four cycles of hydrogen production,the performance remains relatively stable.The tran sient photocurrent response and Nyquist plot experimental results further demonstrate the improvement of carrier separation efficiency in composite catalysts.The semiconductor type(n-type semico nductor)of the single-phase catalyst was determined by the Mott-Schottky test,and the band structure was analyzed.The conductive and valence bands of CN are-0.99 and 1.72 eV,respectively,and the conduction and valence bands of Co-MOF are-1.85 and 1.33 eV,respectively.Th e mechanism of the photocatalytic reaction can be inferred,that is,Z-type heterojunction is formed between CN an d Co-MOF,and NSCQDs was used as cocatalyst.

Is Process as Important as Outcome？ Empirical Evidence from the Chinese Context

Although the relationship between pay level and pay satisfaction has been verified as one of the most robust relationships in the literature of pay satisfaction, scholars have not yet reached an agreement regarding its underlying mechanisms. The present paper aims to investigate the link between absolute pay level and pay level satisfaction by introducing both distributive and procedural fairness perceptions as mediators in accordance with fairness heuristic perspective. A sample of 298 employees from various types of organizations completed a survey, and a series of models were compared to evaluate if the hypothesized theoretical model fits our data. We also constructed bias-corrected bootstrap confidence intervals to test the hypothesized mediation effects. The suggested theoretical model is supported, and future research directions are discussed.

Transformati on al leadership and employee voice: an affective perspective

This study aims to examine the effect of transformational leadership on employee voice behavior. Specifically, this study investigates the mediating role of positive affect in linking sformational leadership with voice and the moderating role of over-qualification in influencing the mediation of positive affect. In particular, a twowave survey was con ducted. A sample of 271 participa nts complete a questionnaire measuring tran sformati on al leadership, positive affect and various demographic variables in the first wave. Then, they complete a second questionnaire measuring perceived over-qualification and voice behavior 2 weeks later. We conduct a series of multiple regression analyses to test the model. The results show that positive affect partially mediated the relationship between transformational leadership and employee voice behavior. Over-qualification moderated the relationship between positive affect and voice. In addition, moderated mediation analyses reveal that positive affect mediates the relationship between transformational leadership and voice only for highly over-qualified employees. This study investigates a new method linking transformational leadership and voice from an affective perspective by finding the mediating effect of positive affect. We also verify the role of "energized to" process in shaping proactive behaviors. Finally, this study examines the boundary conditions of the effect of positive affect on voice.