A cDNA library with the capacity of 2.0×106 was constructed using mRNA extracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5’ non-coding region and 223 nucleo...A cDNA library with the capacity of 2.0×106 was constructed using mRNA extracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5’ non-coding region and 223 nucleotides of 3’ non-coding region was obtained by random screening. The DNA fragment contains an open reading frame of 1 344 nucleotides and encodes 447 amino acids. Seguence analysis of DNA and deduced polypeptide demonstrated that it encoded for a pea elongation factor 1-alpha (EF-1α). The functional domains of EF-1α is well conserved and EF-1α can be a good candidate for studying molecular evolution.展开更多
文摘A cDNA library with the capacity of 2.0×106 was constructed using mRNA extracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5’ non-coding region and 223 nucleotides of 3’ non-coding region was obtained by random screening. The DNA fragment contains an open reading frame of 1 344 nucleotides and encodes 447 amino acids. Seguence analysis of DNA and deduced polypeptide demonstrated that it encoded for a pea elongation factor 1-alpha (EF-1α). The functional domains of EF-1α is well conserved and EF-1α can be a good candidate for studying molecular evolution.
