[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model a...[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model and GGE biplot based on data from a 2-year regional test and a 1-year production test.[Results]The analysis of the AMMI model for the 2-year regional test indicated that Chuanmai 618 had a moderate yield and good stability.During the production test,Chuanmai 618 had an average yield of 450.52 kg/666.7 m^(2),an effective spike of 235700 spike/666.7 m^(2),a 1000-seed weight of 47.93 g,and a kernel number per spike of 47.28.The AMMI analysis sequencing graph showed that the varieties were ranked in the following order:Zhongkemai 1816>Chuanmai 618>Shumai 1958>Chuanyu 42>Mianmai 367>Xikemai 5518.According to the GE analysis,Chuanmai 618 had comparative advantages.[Conclusions]The new wheat variety Chuanmai 618 is a high-quality disease-resistant variety with good yield and stability.展开更多
Background: Insulin-like factor 2(IGF2) plays an important role in embryonic growth process by modulating intermediary metabolism and cell proliferation. Folic acid is involved in one carbon metabolism and contribu...Background: Insulin-like factor 2(IGF2) plays an important role in embryonic growth process by modulating intermediary metabolism and cell proliferation. Folic acid is involved in one carbon metabolism and contributes to DNA methylation which is related to gene expression. The purpose of this study was to explore whether folic acid could regulate IGF2 expression via epigenetic mechanism and further promote embryonic growth of new-hatched broilers.Methods: In the present study, 360 fertile eggs were selected and randomly assigned to four treatments. On11 embryonic day of incubation(E11), 0, 50, 100 and 150 μg folic acid were injected into eggs respectively.After hatched, growth performance of broilers were calculated. Hepatic IGF2 expression, methylation level and chromatin structure of promoter region were analyzed.Results: Results have showed that IGF2 expression was up-regulated in 150 μg folic acid group(P 〈 0.05) and other two dose of folic acid did not affect gene expression(P 〉 0.05). Meanwhile, methylation level of IGF2 promoter were lower in 100 and 150 μg groups, which was consistent with lower expression of DNA methyltransferase1(DNMT1)(P 〈 0.05). What's more, chromatin looseness of IGF2 promoter was higher in 150 μg group than control group(P 〈 0.05). Further, birth weight(BW), liver and bursa index of new-hatched chickens in 150 μg folic acid group were higher than the other groups(P 〈 0.05). There were positive correlations between hepatic IGF2 expression and BW and organs index(P 〈 0.05).Conclusion: In conclusion, our data have demonstrated that 150 μg folic acid injection on E11 could up-regulate IGF2 expression by modulating DNA hypomethylation and improving chromatin accessibility in the gene promoter region,and ulteriorly facilitate embryonic growth and organ development of broilers.展开更多
基金Sichuan"14 th Five-Year Plan"Wheat Breeding Tackling Project(2021YFYZ0002)Sichuan Provincial Financial Special Project(2021ZYGG-003)+2 种基金Sichuan Biological Breeding Major Science and Technology Program(2022ZDZX0014,2022ZDZX0016)Sichuan Science and Technology Program(2022JDRC0113,2022YFSY0015)Modern Discipline Construction Promotion Project of Sichuan Academy of Agricultural Sciences(2021XKJS005).
文摘[Objectives]The paper was to analyze the yield characteristics of a new high-quality disease-resistant wheat variety Chuanmai 618.[Methods]The yield characteristics of Chuanmai 618 were analyzed using the AMMI model and GGE biplot based on data from a 2-year regional test and a 1-year production test.[Results]The analysis of the AMMI model for the 2-year regional test indicated that Chuanmai 618 had a moderate yield and good stability.During the production test,Chuanmai 618 had an average yield of 450.52 kg/666.7 m^(2),an effective spike of 235700 spike/666.7 m^(2),a 1000-seed weight of 47.93 g,and a kernel number per spike of 47.28.The AMMI analysis sequencing graph showed that the varieties were ranked in the following order:Zhongkemai 1816>Chuanmai 618>Shumai 1958>Chuanyu 42>Mianmai 367>Xikemai 5518.According to the GE analysis,Chuanmai 618 had comparative advantages.[Conclusions]The new wheat variety Chuanmai 618 is a high-quality disease-resistant variety with good yield and stability.
基金supported by the Natural Science Foundation of China (No. 31272464)the Program for New Century Excellent Talents (NCET-12-0476)the Program for Shaanxi Science & Technology (2014 K01-18-02, 2015NY149, 2015KTCQ02-19)
文摘Background: Insulin-like factor 2(IGF2) plays an important role in embryonic growth process by modulating intermediary metabolism and cell proliferation. Folic acid is involved in one carbon metabolism and contributes to DNA methylation which is related to gene expression. The purpose of this study was to explore whether folic acid could regulate IGF2 expression via epigenetic mechanism and further promote embryonic growth of new-hatched broilers.Methods: In the present study, 360 fertile eggs were selected and randomly assigned to four treatments. On11 embryonic day of incubation(E11), 0, 50, 100 and 150 μg folic acid were injected into eggs respectively.After hatched, growth performance of broilers were calculated. Hepatic IGF2 expression, methylation level and chromatin structure of promoter region were analyzed.Results: Results have showed that IGF2 expression was up-regulated in 150 μg folic acid group(P 〈 0.05) and other two dose of folic acid did not affect gene expression(P 〉 0.05). Meanwhile, methylation level of IGF2 promoter were lower in 100 and 150 μg groups, which was consistent with lower expression of DNA methyltransferase1(DNMT1)(P 〈 0.05). What's more, chromatin looseness of IGF2 promoter was higher in 150 μg group than control group(P 〈 0.05). Further, birth weight(BW), liver and bursa index of new-hatched chickens in 150 μg folic acid group were higher than the other groups(P 〈 0.05). There were positive correlations between hepatic IGF2 expression and BW and organs index(P 〈 0.05).Conclusion: In conclusion, our data have demonstrated that 150 μg folic acid injection on E11 could up-regulate IGF2 expression by modulating DNA hypomethylation and improving chromatin accessibility in the gene promoter region,and ulteriorly facilitate embryonic growth and organ development of broilers.