In modern warfare,unpowered glide munitions,represented by JDAM,are widely used.Accurately predicting the future trajectory of such targets is crucial for intercepting them.This paper proposes a future point predictio...In modern warfare,unpowered glide munitions,represented by JDAM,are widely used.Accurately predicting the future trajectory of such targets is crucial for intercepting them.This paper proposes a future point prediction method for unpowered gliding targets based on attitude computation.By estimating the current state of the target,we derive the target’s attitude coordinate system.Subsequently,the paper analyzes the forces acting on the target and updates the state transition matrix,ultimately calculating the future position of the target.Experimental results show that,compared to traditional methods,this approach improves the accuracy of future point predictions by 9%to 45%.展开更多
Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory du...Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.展开更多
文摘In modern warfare,unpowered glide munitions,represented by JDAM,are widely used.Accurately predicting the future trajectory of such targets is crucial for intercepting them.This paper proposes a future point prediction method for unpowered gliding targets based on attitude computation.By estimating the current state of the target,we derive the target’s attitude coordinate system.Subsequently,the paper analyzes the forces acting on the target and updates the state transition matrix,ultimately calculating the future position of the target.Experimental results show that,compared to traditional methods,this approach improves the accuracy of future point predictions by 9%to 45%.
基金supported by the National Development and Reform Commission of China(No.20111158)。
文摘Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.
