3,4-Dihydroxy L-phenylalanine(L-DOPA)is considered a potent drug for the treatment of Parkinson disease.Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the hi...3,4-Dihydroxy L-phenylalanine(L-DOPA)is considered a potent drug for the treatment of Parkinson disease.Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of L-DOPA.Screenings of critical components were completed by using a Plackett–Burman design,while further optimization was carried out using the Box–Behnken design.The optimized factor levels predicted by the model were pH 6.1,1.659 g L^(-1)yeast extract,1.491 g L^(-1)L-tyrosine and 0.0290 g L^(-1)CuSO4.The predicted yield of L-DOPA with these levels was 1.319 g L^(-1),while actual yield obtained was 1.273 g L^(-1).The statistical analysis revealed that model is significant with F value 19.55 and R2 value 0.9514.This process resulted in a 3.594-fold increase in the yield of LDOPA.L-DOPA was confirmed by HPTLC and HPLC analysis.Thus,Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of L-DOPA.展开更多
文摘3,4-Dihydroxy L-phenylalanine(L-DOPA)is considered a potent drug for the treatment of Parkinson disease.Physical and nutritional parameters where optimized by using Yarrowia lipolytica-NCIM 3450 to accomplished the highest production of L-DOPA.Screenings of critical components were completed by using a Plackett–Burman design,while further optimization was carried out using the Box–Behnken design.The optimized factor levels predicted by the model were pH 6.1,1.659 g L^(-1)yeast extract,1.491 g L^(-1)L-tyrosine and 0.0290 g L^(-1)CuSO4.The predicted yield of L-DOPA with these levels was 1.319 g L^(-1),while actual yield obtained was 1.273 g L^(-1).The statistical analysis revealed that model is significant with F value 19.55 and R2 value 0.9514.This process resulted in a 3.594-fold increase in the yield of LDOPA.L-DOPA was confirmed by HPTLC and HPLC analysis.Thus,Yarrowia lipolytica-NCIM 3450 has potential to be a new source for the production of L-DOPA.
