Distribution of pathogenic bacteria and antimicrobial sensitivity of eye infections in Suzhou

AIM:To investigate the types of bacteria in patients with eye infections in Suzhou and their drug resistance to commonly used antibacterial drugs.METHODS:The clinical data of 155 patients were retrospectively collected in this study,and the pathogenic bacteria species and drug resistance of each pathogenic bacteria were analyzed.RESULTS:Among the 155 patients(age from 12 to 87 years old,with an average age of 57,99 males and 56 females)with eye infections(160 eyes:74 in the left eye,76 in the right eye and 5 in both eyes,all of which were exogenous),71(45.81%)strains were gram-positive bacteria,23(14.84%)strains were gram-negative bacteria and 61(39.35%)strains were fungi.Gram-positive bacteria were highly resistant to penicillin and erythromycin(78.87%and 46.48%respectively),but least resistant to vancomycin at 0.Gram-negative bacteria were highly resistant to cefoxitin and compound sulfamethoxazole(100%and 95.65%respectively),but least resistant to meropenem at 0.Comparison of the resistance of gram-positive and gram-negative bacteria to some drugs revealed statistically significant differences(P<0.05)in the resistance of both to cefoxitin,cotrimoxazole,levofloxacin,cefuroxime,ceftriaxone and ceftazidime,and both had higher rates of resistance to gram-negative bacteria than to gram-positive bacteria.The distribution of bacterial infection strains showed that Staphylococcus epidermidis was the most common strain in the conjunctiva,cornea,aqueous humor or vitreous body and other eye parts.Besides,Fusarium and Pseudomonas aeruginosa were also among the most common strains of conjunctival and corneal infections.CONCLUSION:Gram-positive bacteria are the dominant bacteria in eye infections,followed by gram-negative bacteria and fungi.Considering the resistance of gramnegative bacteria to multiple drugs,monitoring of bacteria should be strengthened in eye bacterial infections for effective prevention and control to reduce complications caused by eye infections.

大别山黄杜鹃根茎叶花内参基因的表达稳定性评价

黄杜鹃(Rhododendron molle G. Don)是中国野生杜鹃花资源中极具观赏价值和药用价值的特有物种,其野生资源在大别山区零散分布,因此对其稳定内参基因的评估和筛选对黄杜鹃分子生物学和遗传学的研究至关重要。本研究以黄杜鹃根、茎、叶、花为试验材料,采用实时荧光定量PCR技术,分析RmRPL3-1、RmNAC-1、RmCOX1-4、RmPDS-1、RmUBQ-6、RmL10-4等候选内参基因在不同器官组织中的表达稳定性情况,并基于GeNorm、Normfinder和Best-Keeper软件分析荧光定量PCR的结果,筛选最优的内参基因。结果表明黄杜鹃不同组织中最稳定表达的内参基因为RmCOX1-4和RmUBQ-6的组合。本研究筛选的最稳定的内参基因将为黄杜鹃后续的基因表达研究提供校准依据。