Effects of gentiana scabra bage on expression of hepatic type Ⅰ,Ⅲ collagen proteins in Paragonimus skrjabini rats with liver fibrosis

Objective:To exploit- the effects of gentiana scabra bage on the expression of hepatic collagen proteins in Paragonimus skrjabini rats with liver fibrosis.Methods:Immunohistochemical technique was used to observe the changes of content of hepatic type Ⅰ,Ⅲ collagen proteins in Paragonimus skrjabini rats with Liver fibrosis before and after the gentiana scabra bage treatmeat.Results:Comparing with the model group,changes of hepatic tvpe Ⅰ and type Ⅲ collagen proteins in gentiana scabra bage treated group were significantly weakened.Conclusions:Gentiana scabra bage treatment can reduce the content of hepatic type Ⅲ and type Ⅰ collagen protein significantly in Paragonimus skrjabini rats with liver fibrosis,thereby,playing the role against hepatic fibrosis.

Protease-activated receptor(PAR)1, PAR2 and PAR4 expressions in esophageal squamous cell carcinoma

Here,we used reverse transcription-PCR(RT-PCR) and western blot to detect protease-activated receptor(PAR) 1,PAR 2 and PAR 4 expression in cancer tissues and cell lines of esophageal squamous cell carcinoma,and investigated the co-relationship between PAR expression and clinic-pathological data for esophageal cancer.The methylation of PAR4 gene promoter involved in esophageal carcinoma was also analyzed.By comparing the mRNA expressions of normal esophageal tissue and human esophageal epithelial cells(HEEpiC),we found that among the 28 cases of esophageal squamous cell carcinoma,PAR1(60%) and PAR2(71%) were elevated in 17 and 20 cases,respectively,and PAR4(68%) expression was lowered in 19 cases.Whereas,in human esophageal squamous cells(TE-1 and TE-10),PAR1 and PAR2 expression was increased but PAR4 was decreased.Combined with clinical data,the expression of PAR1 in poorly differentiated(P=0.016) and middle and lower parts of the esophagus(P=0.016) was higher; expression of PAR4 in poorly differentiated carcinoma was lower(P=0.049).Regarding TE-1 and TE-10 protein expression,we found that in randomized esophageal carcinoma,PAR1(P=0.027) and PAR2(P=0.039) expressions were increased,but lowered for PAR4(P=0.0001).In HEEpiC,TE-1,TE-10,esophageal and normal esophagus tissue samples(case No.7),the frequency of methylation at the 19 CpG loci of PAR4 was 35.4%,95.2%,83.8%,62.6% and 48.2%,respectively.Our results indicate that the expression of PAR1 and PAR2 in esophageal squamous cell carcinoma is increased but PAR4 is decreased.Hypermethylation of the promoter of the PAR4 gene may contribute to reduced expression of PAR4 in esophageal squamous cell carcinoma.

Primary pancreatic lymphoma: Report of six cases

瞄准：在临床的实践提高主要胰腺的淋巴瘤(PPL ) 的识别。方法：临床的演讲，成像特征和 PPL 病人的病理学的特征的回顾的评论被介绍，以及他们的诊断和治疗，在有文学评论的联合。结果：组织学的诊断被 EUS-FNA 被外科并且在二个病人在四个病人做。六个 PPL 病人(5 男性和 1 女性；年龄变化， 16-65 年；吝啬的年龄， 46 年) 有症状的持续时间二个星期到三个月。主要主要症状不特征，是腹的疼痛，腹的群众，重量损失，黄疸，恶心并且呕吐。病人之一开发了尖锐胰腺炎。在一个病人，浆液 CA19-9 的水平是 76.3 microg/L。腹的 CT 扫描证明六个肿瘤中的三个位于胰的头，二在里面身体和尾巴，和在整个胰的身体。在在四种情况中的胰的肿瘤的直径是超过 6 厘米，与同类的密度和不清楚的边阶。提高的 CT 扫描证明仅仅肿瘤边稍微被提高。胰腺的管不规则地在其肿瘤位于胰腺的头和身体的二种情况中被缩小，在哪个内视镜后退 cholangiopancreatography (ERCP ) 证明近似片断稍微被扩大。二个病人经历了惠普尔操作，一个病人经历了胰切除术，并且另一个病人经历了起作用的胆汁的解压缩。PPL 在舞台我在 2 个病人并且在在 4 个病人的阶段 II E 的 E 根据安乔木分类系统。B 房间 non-Hodgkin 的淋巴瘤的诊断在所有病人被成为组织病理学说的联盟者。所有六个病人经历了全身的化疗，其中一也与鲸鱼群妈辐射线测定被对待。一个病人二个星期在诊断以后死了，二个病人失去了后续，收到的化疗熬过 49 和 37 瞬间的二个病人，和留下的病人仍然是活着的 21 瞬间，在诊断和治疗以后。结论：PPL 是从胰腺的实质发源的额外的节的淋巴瘤的一种稀罕形式。临床并且成像调查结果另外不在胰腺的淋巴瘤和胰腺的癌症的区别是特定的，它值得注意。胰的指导 EUS 的好针的渴望(EUS-FNA ) 象先进免疫一样要求富有经验的 cytopathologists 在组织的小数量上获得最后的诊断的组织化学的试金。外科和辅助化疗或放射疗法能生产相当好的结果。