AIM To investigate the clinicopathological significance of progesterone receptor membrane component 1(PGRMC1) and PGRMC2 in hepatocellular carcinoma(HCC). METHODS We performed immunohistochemical staining to evaluate ...AIM To investigate the clinicopathological significance of progesterone receptor membrane component 1(PGRMC1) and PGRMC2 in hepatocellular carcinoma(HCC). METHODS We performed immunohistochemical staining to evaluate the estrogen receptor(ER), progesterone receptor(PR), PGRMC1, and PGRMC2 in a clinical cohort consisting of 89 paired HCC and non-tumor liver samples. We also analyzed HCC data(n = 373) from The Cancer Genome Atlas(TCGA). We correlated the expression status of PGRMC1 and PGRMC2 with clinicopathological indicators and the clinical outcomes of the HCC patients. We knocked down or overexpressed PGRMC1 in HCC cell lines to evaluate its biological significance in HCC cell proliferation, differentiation, migration, and invasion. RESULTS We found that few HCC cases expressed ER(5.6%) and PR(4.5%). In contrast, most HCC cases expressed PGRMC1(89.9%) and PGRMC2(100%). PGRMC1 and PGRMC2 exhibited significantly lower expression in tumor tissue than in non-tumor tissue(P < 0.001). Lower PGRMC1 expression in HCC was significantly associated with higher serum alpha-fetoprotein expression(P = 0.004), poorer tumor differentiation(P = 0.045) and liver capsule penetration(P = 0.038). Low PGRMC1 expression was an independent predictor for worse disease-free survival(P = 0.002, HR = 2.384,CI: 1.377-4.128) in our cases, as well as in the TCGA cohort(P < 0.001, HR = 2.857, CI: 1.781-4.584). The expression of PGRMC2 did not relate to patient outcome. PGRMC1 knockdown promoted a poorly differentiated phenotype and proliferation of HCC cells in vitro, while PGRMC1 overexpression caused the opposite effects.CONCLUSION PGRMC1 is a non-classical hormonal receptor that negatively regulates hepatocarcinogenesis. PGRMC1 down-regulation is associated with progression of HCC and is a poor prognostic indicator.展开更多
BACKGROUND There has been no report to use camrelizumab with chemotherapy for advanced bladder cancer patients with positive programmed death-ligand 1(PD-L1)expression and high tumor mutational burden(TMB).More effect...BACKGROUND There has been no report to use camrelizumab with chemotherapy for advanced bladder cancer patients with positive programmed death-ligand 1(PD-L1)expression and high tumor mutational burden(TMB).More effective predictors of bladder cancer immunotherapy have yet to be explored,and the combination of multiple factors may be more predictive than a single factor.CASE SUMMARY We report the case of a 74-year-old male patient with recurrent metastatic bladder cancer,which demonstrated positive PD-L1 expression and high TMB.The immune checkpoint inhibitor camrelizumab was administered to the patient in combination with gemcitabine and cisplatin.The patient achieved a partial response with a progression-free survival of 11 mo.CONCLUSION This is the first report to use camrelizumab with chemotherapy for advanced bladder cancer patients with positive PD-L1 expression and high TMB.展开更多
The views of the unities between heaven and man and between form and spirit in The Yellow Emperor’s Classic of Internal Medicine essentially reflect the mutual influence of physical and psychological factors.In today...The views of the unities between heaven and man and between form and spirit in The Yellow Emperor’s Classic of Internal Medicine essentially reflect the mutual influence of physical and psychological factors.In today’s social environment,psychological and social factors have become important factors that affect the incidence of male asthenospermia.Therefore,the treatment of asthenospermia focuses more on the principle of simultaneous treatment of mind and body.With the development of modern biological-psychological-social medicine,the characteristics and advantages of psychosomatic medicine of traditional Chinese medicine are more prominent,which may provide new ideas for the diagnosis and treatment of asthenospermia.展开更多
基金Supported by the Ministry of Science and Technology,No.NSC102-2320-B-006-011.,No.MOST103-2320-B-006-021-MY2,and No.MOST105-2320-B-006-033 to Tsai HWNational Cheng Kung University Hospital,Taiwan,No.NCKUH-10406002 and No.NCKUH-10509001 to Tsai HW
文摘AIM To investigate the clinicopathological significance of progesterone receptor membrane component 1(PGRMC1) and PGRMC2 in hepatocellular carcinoma(HCC). METHODS We performed immunohistochemical staining to evaluate the estrogen receptor(ER), progesterone receptor(PR), PGRMC1, and PGRMC2 in a clinical cohort consisting of 89 paired HCC and non-tumor liver samples. We also analyzed HCC data(n = 373) from The Cancer Genome Atlas(TCGA). We correlated the expression status of PGRMC1 and PGRMC2 with clinicopathological indicators and the clinical outcomes of the HCC patients. We knocked down or overexpressed PGRMC1 in HCC cell lines to evaluate its biological significance in HCC cell proliferation, differentiation, migration, and invasion. RESULTS We found that few HCC cases expressed ER(5.6%) and PR(4.5%). In contrast, most HCC cases expressed PGRMC1(89.9%) and PGRMC2(100%). PGRMC1 and PGRMC2 exhibited significantly lower expression in tumor tissue than in non-tumor tissue(P < 0.001). Lower PGRMC1 expression in HCC was significantly associated with higher serum alpha-fetoprotein expression(P = 0.004), poorer tumor differentiation(P = 0.045) and liver capsule penetration(P = 0.038). Low PGRMC1 expression was an independent predictor for worse disease-free survival(P = 0.002, HR = 2.384,CI: 1.377-4.128) in our cases, as well as in the TCGA cohort(P < 0.001, HR = 2.857, CI: 1.781-4.584). The expression of PGRMC2 did not relate to patient outcome. PGRMC1 knockdown promoted a poorly differentiated phenotype and proliferation of HCC cells in vitro, while PGRMC1 overexpression caused the opposite effects.CONCLUSION PGRMC1 is a non-classical hormonal receptor that negatively regulates hepatocarcinogenesis. PGRMC1 down-regulation is associated with progression of HCC and is a poor prognostic indicator.
文摘BACKGROUND There has been no report to use camrelizumab with chemotherapy for advanced bladder cancer patients with positive programmed death-ligand 1(PD-L1)expression and high tumor mutational burden(TMB).More effective predictors of bladder cancer immunotherapy have yet to be explored,and the combination of multiple factors may be more predictive than a single factor.CASE SUMMARY We report the case of a 74-year-old male patient with recurrent metastatic bladder cancer,which demonstrated positive PD-L1 expression and high TMB.The immune checkpoint inhibitor camrelizumab was administered to the patient in combination with gemcitabine and cisplatin.The patient achieved a partial response with a progression-free survival of 11 mo.CONCLUSION This is the first report to use camrelizumab with chemotherapy for advanced bladder cancer patients with positive PD-L1 expression and high TMB.
基金2021 Hubei University of Chinese Medicine Research Team Building ProjectDocument of Wuhan Health Committee(Wuwei[2019]No.17)the fourth batch of national research and training projects for excellent talents of traditional Chinese medicine(zymyjf[2017]No.24).
文摘The views of the unities between heaven and man and between form and spirit in The Yellow Emperor’s Classic of Internal Medicine essentially reflect the mutual influence of physical and psychological factors.In today’s social environment,psychological and social factors have become important factors that affect the incidence of male asthenospermia.Therefore,the treatment of asthenospermia focuses more on the principle of simultaneous treatment of mind and body.With the development of modern biological-psychological-social medicine,the characteristics and advantages of psychosomatic medicine of traditional Chinese medicine are more prominent,which may provide new ideas for the diagnosis and treatment of asthenospermia.
