瞄准：为了测试交付为改善作为一个 DNA 疫苗的助手编码 IL-15 的一个原生质标志的可行性，有免疫力的回答由肝炎 B 核心基因 DNA 疫苗导致了。方法：我们使用了 RT-PCR 开发 IL-15 表示构造的基于的策略。我们首先证实基因能由于 IL-15...瞄准：为了测试交付为改善作为一个 DNA 疫苗的助手编码 IL-15 的一个原生质标志的可行性，有免疫力的回答由肝炎 B 核心基因 DNA 疫苗导致了。方法：我们使用了 RT-PCR 开发 IL-15 表示构造的基于的策略。我们首先证实基因能由于 IL-15 的差的表示在埃希氏杆菌属关口 i 被表示。然后， IL-15 原生质标志表示产品的简历活动被 CTLL-2 增长试金识别。从每 IL-15 的 DNA 的 100 微克真核细胞的表示原生质标志和怀有编码 HBV 核心基因(缩短的 pHBc144 ) 的 N 终点的 144 氨基酸的 DNA 的 recombinant 原生质标志习惯于共同使免疫 C57 BL/6 老鼠。anti-HBcIgG 的效价被 ELISA 检测，抗原特定的 CD8 (+) T 房间(CD8 (+) IFN-gamma (+) T 房间) 被在不同时间点染色的细胞内部的 cytokine 检测。结果：在由 pIL-15 和老鼠的抗原特定的 CD8 (+) 房间逐渐地增加了的 pHBc144 DNA 疫苗的合作免疫以后，有免疫力的反应的第一座山峰出现了 14 d 以后，然后，抗原特定的 CD8 (+) T 房间的数字逐渐地减少了并且在 3 瞬间在稳定的水平维持了。在增加以后，抗原特定的 CD8 (+) T 房间的数字到达了第二座山峰与以后的 10 d 一第一山峰双，然后，抗原特定的 CD8 (+) T 房间的数字慢慢地减少了。一个基因助手没在体液的有免疫力的回答上有重要效果的 IL-15 由肝炎 B 核心基因 DNA 疫苗导致了，但是增加了抗原特定的 CD8 (+) T 房间由肝炎 B 核心基因 DNA 疫苗导致了的记忆。结论：HBc Ag 1-144 氨基酸构造的 DNA 疫苗导致交付 plasmid 的 IL-15 提高的有效房间免疫，和 cytokine CD8 (+) T 房间的长寿。展开更多
CO poisoning is one of the obstacles for platinum catalysts toward the electro-catalysis process for proton exchange membrane fuel cell(PEMFC)or direct methanol fuel cell(DMFC).Herein,we aim to weaken the CO poisoning...CO poisoning is one of the obstacles for platinum catalysts toward the electro-catalysis process for proton exchange membrane fuel cell(PEMFC)or direct methanol fuel cell(DMFC).Herein,we aim to weaken the CO poisoning on Pt by varying the cluster sizes and supports via doping graphene with B and N based on DFT+D3 calculations.展开更多
To obtain environmentally friendly,integrated and miniaturized gas sensors for the increasing request for the Internet of Things industry and other relative areas,the ultra-thin CoO_(x)/Zn O heterogeneous film with ac...To obtain environmentally friendly,integrated and miniaturized gas sensors for the increasing request for the Internet of Things industry and other relative areas,the ultra-thin CoO_(x)/Zn O heterogeneous film with active interfacial sites was in-situ deposited on micro-electro-mechanical systems(MEMS)as H_(2)S sensor.Atomic layer deposition(ALD)was employed to in-situ fabricate the uniform Zn O thin film.ALD CoO_(x)was deposited on ZnO surface to obtain CoO_(x)/Zn O heterojunction and active interfacial sites.The ultra-thin film(20 nm)with 50 ALD Co O_(x)decorated on 250 ALD Zn O displays excellent sensing performance,including very high response(4.45@200×10^(-9))and selectivity to H_(2)S with a limit of detection(LOD)of 0.38×10^(-9),long-term sensing stability,high response/recovery performance(7.5 s/15.7 s)and mechanical strength at 230。C.Reasons for the high sensing performance of CoO_(x)/Zn O have been confirmed by series of characterizations and density functional theory(DFT)calculation.Heterojunction film thickness with Debye length,the oxygen vacancies and the synergistic effect of active interfacial sites are main reasons for the high sensing performance.The strategy by fabrication of CoO_(x)/Zn O heterogeneous film within Debye length and employing synergistic effect of active interfacial sites offers a promising route for the design of environmentally friendly gas sensors.Furthermore,the ALD technique offers a facile in-situ strategy and high-throughput fabrication of MEMS gas sensors.展开更多
Owing to the wide range and low cost of sodium resources,sodium-ion batteries(SIBs)have received extensive attention and research.Metal sulfides with high theoretical capacity are used as promising anode materials for...Owing to the wide range and low cost of sodium resources,sodium-ion batteries(SIBs)have received extensive attention and research.Metal sulfides with high theoretical capacity are used as promising anode materials for SIBs.This paper presents the electrochemical performance of the binder-free NiS_(2)nanosheet arrays grown on stainless steel(SS)substrate(NiS_(2)/SS)using an in situ growth and sulfidation strategy as anode for sodium ion batteries.Owing to the close connection between the NiS_(2)nanosheet arrays and the SS current collector,the NiS_(2)/SS anode demonstrates high rate capability with a reversible capacity of 492.5 mAh·g^(-1)at 5.0C rate.Such rate capability is superior to that of NiS_(2)nanoparticles(NiS_(2)/CMC:41.7 mAh·g^(-1)at 5.0C,NiS_(2)/PVDF:7.3 mAh·g^(-1)at 5.0C)and other Ni sulfides(100–450 mAh·g^(-1)at 5.0C)reported.Furthermore,the initial reversible specific capacity and Coulombic efficiency of NiS_(2)/SS are 786.5 mAh·g^(-1)and 81%,respec-tively,demonstrating a better sodium storage ability than those of most NiS_(2)anodes reported for SIBs.In addition,the amorphization and conversion mechanism during the sodiation/desodiation process of NiS_(2)are proposed after investigation by in situ X-ray diffraction(XRD)measurements of intermediate products at successive charge/discharge stages.展开更多
基金supported by Research Grant Council(C6011-20G,16308420,and N_HKUST610/17)of the Hong Kong Special Administrative Regionthe support from the Research Grants Council Postdoctoral Fellowship Scheme(PDFS2021-6S08)。
基金the National High Technology Research and Development Program of China (863 Program), No.G1999054105the National Natural Science Foundation of China, No. 30070693
文摘瞄准：为了测试交付为改善作为一个 DNA 疫苗的助手编码 IL-15 的一个原生质标志的可行性，有免疫力的回答由肝炎 B 核心基因 DNA 疫苗导致了。方法：我们使用了 RT-PCR 开发 IL-15 表示构造的基于的策略。我们首先证实基因能由于 IL-15 的差的表示在埃希氏杆菌属关口 i 被表示。然后， IL-15 原生质标志表示产品的简历活动被 CTLL-2 增长试金识别。从每 IL-15 的 DNA 的 100 微克真核细胞的表示原生质标志和怀有编码 HBV 核心基因(缩短的 pHBc144 ) 的 N 终点的 144 氨基酸的 DNA 的 recombinant 原生质标志习惯于共同使免疫 C57 BL/6 老鼠。anti-HBcIgG 的效价被 ELISA 检测，抗原特定的 CD8 (+) T 房间(CD8 (+) IFN-gamma (+) T 房间) 被在不同时间点染色的细胞内部的 cytokine 检测。结果：在由 pIL-15 和老鼠的抗原特定的 CD8 (+) 房间逐渐地增加了的 pHBc144 DNA 疫苗的合作免疫以后，有免疫力的反应的第一座山峰出现了 14 d 以后，然后，抗原特定的 CD8 (+) T 房间的数字逐渐地减少了并且在 3 瞬间在稳定的水平维持了。在增加以后，抗原特定的 CD8 (+) T 房间的数字到达了第二座山峰与以后的 10 d 一第一山峰双，然后，抗原特定的 CD8 (+) T 房间的数字慢慢地减少了。一个基因助手没在体液的有免疫力的回答上有重要效果的 IL-15 由肝炎 B 核心基因 DNA 疫苗导致了，但是增加了抗原特定的 CD8 (+) T 房间由肝炎 B 核心基因 DNA 疫苗导致了的记忆。结论：HBc Ag 1-144 氨基酸构造的 DNA 疫苗导致交付 plasmid 的 IL-15 提高的有效房间免疫，和 cytokine CD8 (+) T 房间的长寿。
基金financially supported by the National Natural Science Foundation of China(No.21975136)the Open Funds from National Engineering Lab for Mobile Source Emission Control Technology(No.NELMS2020A12)。
文摘CO poisoning is one of the obstacles for platinum catalysts toward the electro-catalysis process for proton exchange membrane fuel cell(PEMFC)or direct methanol fuel cell(DMFC).Herein,we aim to weaken the CO poisoning on Pt by varying the cluster sizes and supports via doping graphene with B and N based on DFT+D3 calculations.
基金financially supported by the National Key Research and Development Program of China(No.2020YFB2008600)the financial support from China Scholarship Council(CSC)。
文摘To obtain environmentally friendly,integrated and miniaturized gas sensors for the increasing request for the Internet of Things industry and other relative areas,the ultra-thin CoO_(x)/Zn O heterogeneous film with active interfacial sites was in-situ deposited on micro-electro-mechanical systems(MEMS)as H_(2)S sensor.Atomic layer deposition(ALD)was employed to in-situ fabricate the uniform Zn O thin film.ALD CoO_(x)was deposited on ZnO surface to obtain CoO_(x)/Zn O heterojunction and active interfacial sites.The ultra-thin film(20 nm)with 50 ALD Co O_(x)decorated on 250 ALD Zn O displays excellent sensing performance,including very high response(4.45@200×10^(-9))and selectivity to H_(2)S with a limit of detection(LOD)of 0.38×10^(-9),long-term sensing stability,high response/recovery performance(7.5 s/15.7 s)and mechanical strength at 230。C.Reasons for the high sensing performance of CoO_(x)/Zn O have been confirmed by series of characterizations and density functional theory(DFT)calculation.Heterojunction film thickness with Debye length,the oxygen vacancies and the synergistic effect of active interfacial sites are main reasons for the high sensing performance.The strategy by fabrication of CoO_(x)/Zn O heterogeneous film within Debye length and employing synergistic effect of active interfacial sites offers a promising route for the design of environmentally friendly gas sensors.Furthermore,the ALD technique offers a facile in-situ strategy and high-throughput fabrication of MEMS gas sensors.
基金the National Natural Science Foundation of China(No.21673051)the Department of Science and Technology of Guangdong Province(No.2019A050510043)the Department of Science and Technology of Zhuhai City,China(No.ZH22017001200059PWC)。
文摘Owing to the wide range and low cost of sodium resources,sodium-ion batteries(SIBs)have received extensive attention and research.Metal sulfides with high theoretical capacity are used as promising anode materials for SIBs.This paper presents the electrochemical performance of the binder-free NiS_(2)nanosheet arrays grown on stainless steel(SS)substrate(NiS_(2)/SS)using an in situ growth and sulfidation strategy as anode for sodium ion batteries.Owing to the close connection between the NiS_(2)nanosheet arrays and the SS current collector,the NiS_(2)/SS anode demonstrates high rate capability with a reversible capacity of 492.5 mAh·g^(-1)at 5.0C rate.Such rate capability is superior to that of NiS_(2)nanoparticles(NiS_(2)/CMC:41.7 mAh·g^(-1)at 5.0C,NiS_(2)/PVDF:7.3 mAh·g^(-1)at 5.0C)and other Ni sulfides(100–450 mAh·g^(-1)at 5.0C)reported.Furthermore,the initial reversible specific capacity and Coulombic efficiency of NiS_(2)/SS are 786.5 mAh·g^(-1)and 81%,respec-tively,demonstrating a better sodium storage ability than those of most NiS_(2)anodes reported for SIBs.In addition,the amorphization and conversion mechanism during the sodiation/desodiation process of NiS_(2)are proposed after investigation by in situ X-ray diffraction(XRD)measurements of intermediate products at successive charge/discharge stages.
