Objective:To explore the status of self-perceived burden(SPB)in primary glaucoma patients and to analyze its influencing factors.Subject and setting:A questionnaire survey was administered to 236 inpatients from a ter...Objective:To explore the status of self-perceived burden(SPB)in primary glaucoma patients and to analyze its influencing factors.Subject and setting:A questionnaire survey was administered to 236 inpatients from a tertiary general hospital and a eye hospital in Tianjin.The investigation was conducted after obtaining informed consent from each participant.Instruments:They were investigated using general data questionnaire,Self-Perceived Burden Scale(SPBS),Medical Coping Modes Questionnaire(MCMQ).Design:A descriptive cross-sectional design was used to gather data in this study.Results:The total SPBS score of primary glaucoma patients was(31.10±9.34)was medium.Regression consults showed that avoidance and surrender coping style,medical burden and right eye vision were the influencing factors of patients’SPB(P<0.05).Conclusion:Patients with primary glaucoma have a relatively heavy SPB,so medical staff should encourage them to actively face it.Tailored strategies in line with the patient’s economic and visual conditions to reduce the SPB.展开更多
Objective:To analysis the research status of the elderly's frailty in China by bibliometric,in order to provide reference for clinical practices and nursing researches.Methods:Search China National Knowledge Infra...Objective:To analysis the research status of the elderly's frailty in China by bibliometric,in order to provide reference for clinical practices and nursing researches.Methods:Search China National Knowledge Infrastructure(CNKI),WanFang Data,CQVIP,China Biology Medicine(CBM)disc to find the studies concerning frailty of older adults in China,and analysis the information of Published year,Provinces,Institutions,Journals,Types,Contents and Funds.Results:A Total of 248 articles were included in this study.From 2011,the numbe of studies about the elderly's frailty was growing rapidly;the province with the largest number of publications was Beijing,with a total of 82 articles;the institution with the largest number of publications was Beijing Hospital,with 20 articles;the journal with the largest number of publications was Chinese Journal of Geriatrics,with 27 articles;the research type with the largest number of articles was cross-sectional study,with 92 articles,whose content was mainly about the factors of frailty.Conclusion:The study on the elderly's frailty was just starting in China.Hoping to carry out interventional studies widely in the future,so as to delay the progress of frailty and improve the quality of life of the elderly.展开更多
Objective:The study aims to investigate the molecular mechanism and druggability of Atractylide I(AT-I)for the treatment of diabetic nephropathy(DN)using network pharmacology,molecular docking,and pharmacokinetic anal...Objective:The study aims to investigate the molecular mechanism and druggability of Atractylide I(AT-I)for the treatment of diabetic nephropathy(DN)using network pharmacology,molecular docking,and pharmacokinetic analysis.Method:Multiple databases and service platforms were used to predict and screen the potential genes of AT-I for the prevention and treatment of DN.Further,gene ontology and kyoto encyclopedia of genes and genomes enrichment analyses were performed on the genes to evaluate the mechanism of AT-I against DN.The protein interaction network was constructed by the STRING database,and the key gene targets of AT-I against DN were screened by Cytoscape software.The binding activity of AT-I with the essential target proteins was analyzed by molecular docking technique.Finally,the pharmacokinetic parameters of AT-I were evaluated to determine its druggability.Result:AT-I has 45 drug action targets intersecting with DN targets,which may be the potential targets of AT-I for the prevention and treatment of DN.The enrichment analysis indicated that gene expression regulation,insulin resistance,hypoxia inducible factor-1 signaling pathway,tumor necrosis factor signaling pathway,and PI3K-Akt signaling pathway determine the biological mechanism of AT-I in the prevention and treatment of DN.Further,network pharmacology and molecular docking results indicated that key genes such as AKT1,MAPK8,EGFR,MAPK1,MMP9,and MTOR bind well with AT-I,while AKT1 is probably the primary target gene of AT-I against DN.Pharmacokinetic analysis showed that AT-I conforms to Lipinski’s rule of five drug-like properties,has good gastrointestinal absorption,can pass the blood-brain barrier,and has superior biological properties.Conclusion:AT-I has desirable druggability and multi-target,multi-pathway,and multi-mechanism activity against DN.This study provides an important basis for future research on the activity of AT-I against DN.展开更多
Objective: Developed the core-matched nanoemulsions(CMNEs) to co-delivery paclitaxel-oleic acid(PTXOA) prodrug and Brucea javanica oil(BJO) for increasing the antitumor effect.Methods: Antitumor effects and mechanism ...Objective: Developed the core-matched nanoemulsions(CMNEs) to co-delivery paclitaxel-oleic acid(PTXOA) prodrug and Brucea javanica oil(BJO) for increasing the antitumor effect.Methods: Antitumor effects and mechanism of PTX-OA/BJO CMNEs that the combination therapy which based on core-matched technology(CMT) were evaluated in vitro and in vivo.Results: The PTX-OA/BJO CMNEs were of nanoscale particle size(108.7 ± 2.3) nm and with entrapment efficiency of >95%. The PTX-OA/BJO CMNEs displayed concentration and time-dependent cytotoxicity against HepG-2 cells and increased G2/M phase block. More importantly, a significant reduction of the tumor volume with no obvious toxicity was observed in nude mice model following administration of PTX-OA/BJO CMNEs compared with the control treated with normal saline(P < 0.05), which suggested the excellent efficacy in vivo. It was further found that the enhanced effectiveness of PTX-OA/BJO CMNEs were associated with the ability of inducing apoptosis of the tumor cells, as well as obviously inhibiting tumor cell proliferation and the activity of TOPOⅡ.Conclusion: Co-encapsulation of two drugs with different mechanisms allows simultaneous interruption of diverse anticancer pathways, resulting in increased therapeutic response and lower toxicity.展开更多
文摘Objective:To explore the status of self-perceived burden(SPB)in primary glaucoma patients and to analyze its influencing factors.Subject and setting:A questionnaire survey was administered to 236 inpatients from a tertiary general hospital and a eye hospital in Tianjin.The investigation was conducted after obtaining informed consent from each participant.Instruments:They were investigated using general data questionnaire,Self-Perceived Burden Scale(SPBS),Medical Coping Modes Questionnaire(MCMQ).Design:A descriptive cross-sectional design was used to gather data in this study.Results:The total SPBS score of primary glaucoma patients was(31.10±9.34)was medium.Regression consults showed that avoidance and surrender coping style,medical burden and right eye vision were the influencing factors of patients’SPB(P<0.05).Conclusion:Patients with primary glaucoma have a relatively heavy SPB,so medical staff should encourage them to actively face it.Tailored strategies in line with the patient’s economic and visual conditions to reduce the SPB.
文摘Objective:To analysis the research status of the elderly's frailty in China by bibliometric,in order to provide reference for clinical practices and nursing researches.Methods:Search China National Knowledge Infrastructure(CNKI),WanFang Data,CQVIP,China Biology Medicine(CBM)disc to find the studies concerning frailty of older adults in China,and analysis the information of Published year,Provinces,Institutions,Journals,Types,Contents and Funds.Results:A Total of 248 articles were included in this study.From 2011,the numbe of studies about the elderly's frailty was growing rapidly;the province with the largest number of publications was Beijing,with a total of 82 articles;the institution with the largest number of publications was Beijing Hospital,with 20 articles;the journal with the largest number of publications was Chinese Journal of Geriatrics,with 27 articles;the research type with the largest number of articles was cross-sectional study,with 92 articles,whose content was mainly about the factors of frailty.Conclusion:The study on the elderly's frailty was just starting in China.Hoping to carry out interventional studies widely in the future,so as to delay the progress of frailty and improve the quality of life of the elderly.
基金supported by the National Student Innovation and Entrepreneurship Training Program(202110114006).
文摘Objective:The study aims to investigate the molecular mechanism and druggability of Atractylide I(AT-I)for the treatment of diabetic nephropathy(DN)using network pharmacology,molecular docking,and pharmacokinetic analysis.Method:Multiple databases and service platforms were used to predict and screen the potential genes of AT-I for the prevention and treatment of DN.Further,gene ontology and kyoto encyclopedia of genes and genomes enrichment analyses were performed on the genes to evaluate the mechanism of AT-I against DN.The protein interaction network was constructed by the STRING database,and the key gene targets of AT-I against DN were screened by Cytoscape software.The binding activity of AT-I with the essential target proteins was analyzed by molecular docking technique.Finally,the pharmacokinetic parameters of AT-I were evaluated to determine its druggability.Result:AT-I has 45 drug action targets intersecting with DN targets,which may be the potential targets of AT-I for the prevention and treatment of DN.The enrichment analysis indicated that gene expression regulation,insulin resistance,hypoxia inducible factor-1 signaling pathway,tumor necrosis factor signaling pathway,and PI3K-Akt signaling pathway determine the biological mechanism of AT-I in the prevention and treatment of DN.Further,network pharmacology and molecular docking results indicated that key genes such as AKT1,MAPK8,EGFR,MAPK1,MMP9,and MTOR bind well with AT-I,while AKT1 is probably the primary target gene of AT-I against DN.Pharmacokinetic analysis showed that AT-I conforms to Lipinski’s rule of five drug-like properties,has good gastrointestinal absorption,can pass the blood-brain barrier,and has superior biological properties.Conclusion:AT-I has desirable druggability and multi-target,multi-pathway,and multi-mechanism activity against DN.This study provides an important basis for future research on the activity of AT-I against DN.
基金supported by the National Natural Science Foundation of China(81403109)Science and Technology Planning Project of Guangdong Province(2014A020210021,2016A020217017)
文摘Objective: Developed the core-matched nanoemulsions(CMNEs) to co-delivery paclitaxel-oleic acid(PTXOA) prodrug and Brucea javanica oil(BJO) for increasing the antitumor effect.Methods: Antitumor effects and mechanism of PTX-OA/BJO CMNEs that the combination therapy which based on core-matched technology(CMT) were evaluated in vitro and in vivo.Results: The PTX-OA/BJO CMNEs were of nanoscale particle size(108.7 ± 2.3) nm and with entrapment efficiency of >95%. The PTX-OA/BJO CMNEs displayed concentration and time-dependent cytotoxicity against HepG-2 cells and increased G2/M phase block. More importantly, a significant reduction of the tumor volume with no obvious toxicity was observed in nude mice model following administration of PTX-OA/BJO CMNEs compared with the control treated with normal saline(P < 0.05), which suggested the excellent efficacy in vivo. It was further found that the enhanced effectiveness of PTX-OA/BJO CMNEs were associated with the ability of inducing apoptosis of the tumor cells, as well as obviously inhibiting tumor cell proliferation and the activity of TOPOⅡ.Conclusion: Co-encapsulation of two drugs with different mechanisms allows simultaneous interruption of diverse anticancer pathways, resulting in increased therapeutic response and lower toxicity.
