A control and data acquisition system was implemented for the recently developed collinear laser spectroscopy setup.This system is dedicated to data recording,storage,processing,monitoring of the beam intensity and en...A control and data acquisition system was implemented for the recently developed collinear laser spectroscopy setup.This system is dedicated to data recording,storage,processing,monitoring of the beam intensity and energy,and visualization of various spectra.In comparison to the conventional resonance nuclear reaction system,the key technique is the precise synchronization of the detected counts with the actual scanning voltage(or probing laser frequency).The functions of the system were tested by measuring the hyperfine structure spectra of stable calcium(e.g.,^(40)Ca^(+))and radioactive potassium(e.g.,^(38)K)in the bunched and continuous modes,respectively.This system will be routinely applied and further improved in subsequent laser spectroscopy experiments on unstable isotopes at the Beijing Radioactive Ion-beam Facility(BRIF).展开更多
Background and Objective:Heparanase-1 (HPA-1) can promote angiogenesis and metastasis of malignant tumors and plays an important role in the genesis and development of tumors.This study was to explore the effects of s...Background and Objective:Heparanase-1 (HPA-1) can promote angiogenesis and metastasis of malignant tumors and plays an important role in the genesis and development of tumors.This study was to explore the effects of specific small interfering RNA (siRNA) targeting HPA-1 combined with heparin on invasiveness of mouse hepatocellular carcinoma cells.Methods:The expression of HPA-1 in Hca-F, Hca-P, and Hepa1-6 cells, which have high, low, and no metastatic potential, respectively, was analyzed by reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis and enzyme-linked immunosorbent assay (ELISA).After transfection with two specific siRNAs targeting HPA-1, siRNA-1 and siRNA-2, and treatment with heparin, invasiveness of Hca-F cells was observed by Matrigel invasion assay.Results:HPA-1 was negative in Hepa1-6 cells while positive in both Hca-F and Hca-P cells.The expression levels of both HPA-1 mRNA and protein were obviously higher in Hca-F cells than in Hca-P cells.HPA-1 proteins could be secreted into culture supernatant of Hca-F and Hca-P cells, and the amount of secreted HPA-1 detected by Western blot analysis was larger in Hca-F cells than in Hca-P cells (1.34±0.02 vs.0.60±0.01, P<0.001), which was consistent with the results of ELISA.Both siRNA-1 and siRNA-2 downregulated the expression of HPA-1 and the siRNA-2 did more efficiently.The number of invasive Hca-F cells treated with siRNA-2 or heparin alone was larger than that of Hca-F cells treated with combination of them (9±1 vs.4±1, P=0.013; 15±2 vs.4±1, P=0.008), but smaller than that of untreated Hca-F cells (9±1 vs.22±2, P=0.006; 15±2 vs.22±2, P=0.026).Conclusion:The combined application of specific siRNA targeting HPA-1 and heparin is more effective in inhibiting the invasiveness of mouse hepatoma cells.展开更多
Collinear laser spectroscopy is a powerful tool for studying the nuclear spins,electromagnetic moments,and charge radii of exotic nuclei.To study the nuclear properties of unstable nuclei at the Beijing Radioactive Io...Collinear laser spectroscopy is a powerful tool for studying the nuclear spins,electromagnetic moments,and charge radii of exotic nuclei.To study the nuclear properties of unstable nuclei at the Beijing Radioactive Ion-beam Facility(BRIF)and the future High Intensity Heavy-ion Accelerator Facility(HIAF),we developed a collinear laser spectroscopy apparatus integrated with an offline laser ablation ion source and a laser system.The overall performance of this state-of-the-art technique was evaluated,and the system was commissioned using a bunched stable ion beam.The high-resolution optical spectra for the 4s ^(2)S_(1/2)→4p^(2)P_(3/2)(D2)ionic transition of ^(40;42;44;48)Ca isotopes were successfully measured.The extracted isotope shifts relative to ^(40)Ca showed excellent agreement with the literature values.This system is now ready for use at radioactive ion beam facilities such as the BRIF and paves the way for the further development of higher-sensitivity collinear resonance ionization spectroscopy techniques.展开更多
The impact of boron on the dissolution and repairing behavior of passive films formed on S31254 super-austenitic stainless steel(SASS)was investigated.SASS was immersed in 0.5 mol/L of H_(2)SO_(4) for 0,2,6,10,and 14 ...The impact of boron on the dissolution and repairing behavior of passive films formed on S31254 super-austenitic stainless steel(SASS)was investigated.SASS was immersed in 0.5 mol/L of H_(2)SO_(4) for 0,2,6,10,and 14 days to explore the evolution of the passive film.The electrochemical impedance spectroscope(EIS),the Mott–Schottky analysis,and X-ray photoelectron spectroscope were utilized to analyze the semiconductor properties and compositions of the passive films.EIS showed a decrease and consequent increase over 14 days;the same pattern was observed for Cr_(2)O_(3) and Cr/Fe.However,the defect density of the passive film exhibited a reverse trend.The variation in film thicknesses indicated that the passive films possessed dissolution and repairing behavior.SASS passive film had a double-layer structure;the outer layer was found to be rich in Fe3+and Cr(OH)3,but low on Mo6+,while the inner layer was rich in Cr_(2)O_(3) and low in Mo4+.The addition of boron increased the corrosion resistance and could promote the efficiency of the passive film repair,likely by promoting the migration of Mox+,which promoted the repairing of the passive film.展开更多
基金supported by the National Natural Science Foundation of China (Nos.12027809,U1967201,11875073,11875074 and 11961141003)National Key R&D Program of China (No.2018YFA0404403)the State Key Laboratory of Nuclear Physics and Technology,Peking University (Nos.NPT2019ZZ02,NPT2020KFY17).
文摘A control and data acquisition system was implemented for the recently developed collinear laser spectroscopy setup.This system is dedicated to data recording,storage,processing,monitoring of the beam intensity and energy,and visualization of various spectra.In comparison to the conventional resonance nuclear reaction system,the key technique is the precise synchronization of the detected counts with the actual scanning voltage(or probing laser frequency).The functions of the system were tested by measuring the hyperfine structure spectra of stable calcium(e.g.,^(40)Ca^(+))and radioactive potassium(e.g.,^(38)K)in the bunched and continuous modes,respectively.This system will be routinely applied and further improved in subsequent laser spectroscopy experiments on unstable isotopes at the Beijing Radioactive Ion-beam Facility(BRIF).
基金Natural Science Foundation of Liaoning Province (No.20072215)
文摘Background and Objective:Heparanase-1 (HPA-1) can promote angiogenesis and metastasis of malignant tumors and plays an important role in the genesis and development of tumors.This study was to explore the effects of specific small interfering RNA (siRNA) targeting HPA-1 combined with heparin on invasiveness of mouse hepatocellular carcinoma cells.Methods:The expression of HPA-1 in Hca-F, Hca-P, and Hepa1-6 cells, which have high, low, and no metastatic potential, respectively, was analyzed by reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis and enzyme-linked immunosorbent assay (ELISA).After transfection with two specific siRNAs targeting HPA-1, siRNA-1 and siRNA-2, and treatment with heparin, invasiveness of Hca-F cells was observed by Matrigel invasion assay.Results:HPA-1 was negative in Hepa1-6 cells while positive in both Hca-F and Hca-P cells.The expression levels of both HPA-1 mRNA and protein were obviously higher in Hca-F cells than in Hca-P cells.HPA-1 proteins could be secreted into culture supernatant of Hca-F and Hca-P cells, and the amount of secreted HPA-1 detected by Western blot analysis was larger in Hca-F cells than in Hca-P cells (1.34±0.02 vs.0.60±0.01, P<0.001), which was consistent with the results of ELISA.Both siRNA-1 and siRNA-2 downregulated the expression of HPA-1 and the siRNA-2 did more efficiently.The number of invasive Hca-F cells treated with siRNA-2 or heparin alone was larger than that of Hca-F cells treated with combination of them (9±1 vs.4±1, P=0.013; 15±2 vs.4±1, P=0.008), but smaller than that of untreated Hca-F cells (9±1 vs.22±2, P=0.006; 15±2 vs.22±2, P=0.026).Conclusion:The combined application of specific siRNA targeting HPA-1 and heparin is more effective in inhibiting the invasiveness of mouse hepatoma cells.
基金supported by the National Natural Science Foundation of China(Nos.12027809,U1967201,11875073,11875074 and 11961141003)National Key R&D Program of China(No.2018YFA0404403)+1 种基金China National Nuclear Corporation(No.FA18000201)the State Key Laboratory of Nuclear Physics and Technology,Peking University(No.NPT2019ZZ02).
文摘Collinear laser spectroscopy is a powerful tool for studying the nuclear spins,electromagnetic moments,and charge radii of exotic nuclei.To study the nuclear properties of unstable nuclei at the Beijing Radioactive Ion-beam Facility(BRIF)and the future High Intensity Heavy-ion Accelerator Facility(HIAF),we developed a collinear laser spectroscopy apparatus integrated with an offline laser ablation ion source and a laser system.The overall performance of this state-of-the-art technique was evaluated,and the system was commissioned using a bunched stable ion beam.The high-resolution optical spectra for the 4s ^(2)S_(1/2)→4p^(2)P_(3/2)(D2)ionic transition of ^(40;42;44;48)Ca isotopes were successfully measured.The extracted isotope shifts relative to ^(40)Ca showed excellent agreement with the literature values.This system is now ready for use at radioactive ion beam facilities such as the BRIF and paves the way for the further development of higher-sensitivity collinear resonance ionization spectroscopy techniques.
基金The research was financially supported by the National Natural Science Foundation of China(Grant Nos.U1860204,51871159 and 52104338)the Natural Science Foundation of Shanxi Province(Grant No.201901D111103).
文摘The impact of boron on the dissolution and repairing behavior of passive films formed on S31254 super-austenitic stainless steel(SASS)was investigated.SASS was immersed in 0.5 mol/L of H_(2)SO_(4) for 0,2,6,10,and 14 days to explore the evolution of the passive film.The electrochemical impedance spectroscope(EIS),the Mott–Schottky analysis,and X-ray photoelectron spectroscope were utilized to analyze the semiconductor properties and compositions of the passive films.EIS showed a decrease and consequent increase over 14 days;the same pattern was observed for Cr_(2)O_(3) and Cr/Fe.However,the defect density of the passive film exhibited a reverse trend.The variation in film thicknesses indicated that the passive films possessed dissolution and repairing behavior.SASS passive film had a double-layer structure;the outer layer was found to be rich in Fe3+and Cr(OH)3,but low on Mo6+,while the inner layer was rich in Cr_(2)O_(3) and low in Mo4+.The addition of boron increased the corrosion resistance and could promote the efficiency of the passive film repair,likely by promoting the migration of Mox+,which promoted the repairing of the passive film.