The microscopic heterogeneity of pore-throat structures in tight sandstone is a crucial parameter for understanding the transport mechanism of fluid flow.In this work,we firstly developed the new procedure to characte...The microscopic heterogeneity of pore-throat structures in tight sandstone is a crucial parameter for understanding the transport mechanism of fluid flow.In this work,we firstly developed the new procedure to characterize the pore size distribution(PSD)and throat size distribution(TSD)by combining the nuclear magnetic resonance(NMR),cast thin section(CTS),and constant-rate mercury injection(CRMI)tests,and used the permeability estimated model to verify the full-scale PSD and TSD.Then,we respectively analyzed the fractal feature of the pore and throat,and characterized the heterogeneity of pores and throats.Finally,we elaborated the effect of the pore and throat heterogeneity on the gas-phase seepage capacity base on the analysis of the simple capillary tube model and gas-flooding experiment.The results showed that(1)The PSD and TSD of the tight sandstone sample ranged from 0.01 to 10 mm and from 0.1 to 57 mm,respectively,mainly contributed by the micropores and mesopores.Meanwhile,the permeability estimated by the PSD and TSD was consistent with the experimental permeability,and relative error was lower than 8%.(2)The PSD and TSD exhibited multifractal characteristics,and singularity strength range,Δα,could be used as the indicator for characterizing the heterogeneity of pore and throat.Furthermore,the throat of the sample showed stronger heterogeneity than that the pore.(3)The throats played an important role for the fluid transport in the tight sandstone,and the effect of the throat heterogeneity on the gas-phase seepage capacity was different under the lower and higher injection pressure.The macropores and micropores maybe respectively become the preferential migration pathways at the lower and higher injection pressure.In the end,the identification plate was established in our paper,and could be described the relationship among the throat heterogeneity,injection pressure,permeability and flow path of the gas phase in the tight sandstone.展开更多
The mechanism underlying acrylamide-induced neurotoxicity remains controversial. Previous studies have focused on acrylamide-induced toxicity in adult rodents, but neurotoxicity in weaning rats has not been investigat...The mechanism underlying acrylamide-induced neurotoxicity remains controversial. Previous studies have focused on acrylamide-induced toxicity in adult rodents, but neurotoxicity in weaning rats has not been investigated. To explore the neurotoxic effect of acrylamide on the developing brain, weaning rats were gavaged with 0, 5, 15, and 30 mg/kg acrylamide for 4 consecutive weeks. No obvious neurotoxicity was observed in weaning rats in the low-dose acrylamide group （5 mg/kg）. However, rats from the moderateand high-dose acrylamide groups （15 and 30 mg/kg） had an abnormal gait. Furthermore, biochemical tests in these rats demonstrated that glutamate concentration was significantly reduced, and ^-aminobutyric acid content was significantly increased and was dependent on acrylamide dose. Immunohis- tochemical staining showed that in the cerebral cortex, γ-aminobutyric acid, glutamic acid decarboxylase and glial fibrillary acidic protein expression increased remarkably in the moderate- and high-dose acrylamide groups. These results indicate that in weaning rats, acrylamide is positively associated with neurotoxicity in a dose-dependent manner, which may correlate with upregulation of γ-aminobutyric acid and subsequent neuronal degeneration after the initial acrylamide exposure.展开更多
Haemangiopericytoma(HPC) is a rare vascular tumor with borderline malignancy, considerable histological variability, and unpredictable clinical and biological behavior. HPC can present a diagnostic challenge because o...Haemangiopericytoma(HPC) is a rare vascular tumor with borderline malignancy, considerable histological variability, and unpredictable clinical and biological behavior. HPC can present a diagnostic challenge because of its indeterminate clinical, radiological, and pathological features. HPC generally presents in adulthood and is equally frequent in both sexes. HPC can arise in any site in the body as a slowly growing and painless mass. The precise cell type origin of HPC is uncertain. One third of HPCs occur in the head and neck areas. Exceptional cases of hemangioblastoma arising outside the head and neck areas have been reported, but little is known about their clinicopathologic and immunohistochemical features. This study reports on a case of a large sacro-anterior HPC in a 65-year-old male.展开更多
Both the band filling effect and Fe/Mo disorder have a close correlation with the physical properties of the double perovskite Ca2FeMoO6. Two series of Ca2FeMoO6and Nd0.3Ca1.7FeMoO6ceramics sintered at(1050℃, 1200℃,...Both the band filling effect and Fe/Mo disorder have a close correlation with the physical properties of the double perovskite Ca2FeMoO6. Two series of Ca2FeMoO6and Nd0.3Ca1.7FeMoO6ceramics sintered at(1050℃, 1200℃, and 1300℃) were specially designed to comparatively investigate the band-filling effect and Fe/Mo disorder on the physical properties of Ca2FeMoO6. The x-ray diffraction indicates that Fe/Mo disorder is sensitive to the sintering temperature. The magnetization behavior is mainly controlled by the Fe/Mo disorder not by the band filling effect, manifested by a close correlation of saturated magnetization(Ms) with the Fe/Mo disorder. Interestingly, magnetoresistance(MR) property of the same composition is dominantly contributed by the grain boundary strength, which can be expressed by the macroscopic resistivity values. However, the band filling effect caused by the Nd-substitution can decrease the spin polarization, and thus suppress the MR performance fundamentally. Contrary to the MR response, the Curie temperature(TC) shows an obvious optimization due to the band filling effect, which increases the carrier density near the Fermi level responsible for the ferromagnetic coupling interaction strengthen. Maybe, our work can provoke further research interests into the correlation of the band-filling effects and Fe/Mo disorder with the physical properties of other Fe/Mo-based double perovskites.展开更多
Pancreatic ductal adenocarcinoma(PDAC) is one of the five most malignant cancer.ZX-1201 is one of the active constituents in Alismatis Rhizoma,a well-known traditional Chinese medi.cine with a wide variety of pharmaco...Pancreatic ductal adenocarcinoma(PDAC) is one of the five most malignant cancer.ZX-1201 is one of the active constituents in Alismatis Rhizoma,a well-known traditional Chinese medi.cine with a wide variety of pharmacological properties including diuretic,anti-hyperlipidemic,anti-atheroscle.rotic,anti-cancer,anti-inflammatory and anti-oxidative activities.We investigated the inhibitory effect of ZX-1201 on pancreatic cancer and the relevant molecular mechanism in vitro and in vivo.ZX-1201 inhibited the growth and metastasis of PANC-1 cells in BALB/c nude mice significantly.ZX-1201 inhibited the function of AQP1 via directly interaction and involved in the reversion process of ZX-1201 on TGF-β_1.CTGF was an important protein in the reversion process of ZX-1201 on TGF-β_1.ZX-1201 inhibited the migration of PANC-1 and CPFAC-1 cells induced by TGF-β_1 in vitro.ZX-1201 reversed the down-regu.lated of epithelial markers and up-regulated of mesenchymal markers,as well as the up-regulated of Snail and p-Smad2/3 induced by TGF-β_1.And ZX-1201 reversed Epithelial-Mesenchymal Transition by down-regulating AQP1 and inhibiting translocation of β-catenin,the promotor of CTGF.According to these,ZX-1201 inhibited the migration of pancreatic cancer cells.We concluded that ZX-1201 inhibited the growth and metastasis of PANC-1 cells in vivo significantly.And AQP1,β-catenin and CTGF were the pivotal proteins in the process of ZX-1201 inhibiting PANC-1 cells migration induced by TGF-β_1.展开更多
In the past two decades,with the increase of smoking population,more and more people are suffering from small cell lung cancer(SCLC).Besides,it is difficult to find an effective way to cure SCLC,since patience can eas...In the past two decades,with the increase of smoking population,more and more people are suffering from small cell lung cancer(SCLC).Besides,it is difficult to find an effective way to cure SCLC,since patience can easily develop drug resistance.On the other hand,with the development of science and technology,people began to study the anti-cancer strategy to increase apoptosis,such as inhibiting the overexpression of survival factors.In these survival factors,BCL-2 family has attracted a lot of attention.BH3-only protein is a member of BCL-2 family and it can directly inhibit the expression of BCL-2 protein,thereby prompting apoptosis.Since the BH3-only protein itself is difficult to become a clinical drug,to find alternatives BH3-only protein-BH3 mimetics is particularly important.Plus,more and more researchers have paid attention on the natural BH3 mimetic since it has less side-effect than artificial BH3 mimetics.To find possible BH3 mimetics,we made a primary screening with this pharma.cophore on a small molecular compounds library via Discovery Studio software.And then MTS assay were introduced to verify the activity of compounds.After that,we use Western Blot and Co-IP meth.ods to test the effect of BH3 mimetics.And finally use CDOCKER to predict the further mechanism on autophagy and apoptosis.In our studies,we found 3 possible BH3 mimetics compounds from 170,000 natural small molecular compounds via pharmacophore-based virtual screening.Furthermore,we dem.onstrated AD23,one of the 3 possible natural BH3 mimetics,induced autophagy and apoptosis simulta.neously in dose-time dependence in SCLC cell line.Finally,we use Molecular Docking to predict the further mechanism on autophagy and apoptosis.We believe our works would provide evidences and clues for the structural optimizing and further study of new drugs in the future.展开更多
DEAR EDITOR,The Pegasidae family,which includes seven species of seamoths,is widely distributed in the temperate and tropical waters of the Indo-Pacific,ranging from South Africa to Hawaii.In this study,we describe a ...DEAR EDITOR,The Pegasidae family,which includes seven species of seamoths,is widely distributed in the temperate and tropical waters of the Indo-Pacific,ranging from South Africa to Hawaii.In this study,we describe a new species of seamoth,Pegasus sinensis sp.nov.,based on morphological and molecular characterization of 14 specimens collected from the surrounding waters of Xiamen City,Fujian Province,China.The new species differs from closely related P.volitans in both the density and color of spots present on the dorsal and lateral body surfaces.展开更多
基金supported by the National Natural Science Foundation of China(No.52071035)Guangdong Major Project of Basic and Applied Basic Research,China(No.2020B0301030006)。
基金financial support from the Youth Science and Technology Innovation Team of Southwest Petroleum University(No.2018CXTD10)the National Natural Science Foundation Project of China(No.51874248 and No.U19B2010).
文摘The microscopic heterogeneity of pore-throat structures in tight sandstone is a crucial parameter for understanding the transport mechanism of fluid flow.In this work,we firstly developed the new procedure to characterize the pore size distribution(PSD)and throat size distribution(TSD)by combining the nuclear magnetic resonance(NMR),cast thin section(CTS),and constant-rate mercury injection(CRMI)tests,and used the permeability estimated model to verify the full-scale PSD and TSD.Then,we respectively analyzed the fractal feature of the pore and throat,and characterized the heterogeneity of pores and throats.Finally,we elaborated the effect of the pore and throat heterogeneity on the gas-phase seepage capacity base on the analysis of the simple capillary tube model and gas-flooding experiment.The results showed that(1)The PSD and TSD of the tight sandstone sample ranged from 0.01 to 10 mm and from 0.1 to 57 mm,respectively,mainly contributed by the micropores and mesopores.Meanwhile,the permeability estimated by the PSD and TSD was consistent with the experimental permeability,and relative error was lower than 8%.(2)The PSD and TSD exhibited multifractal characteristics,and singularity strength range,Δα,could be used as the indicator for characterizing the heterogeneity of pore and throat.Furthermore,the throat of the sample showed stronger heterogeneity than that the pore.(3)The throats played an important role for the fluid transport in the tight sandstone,and the effect of the throat heterogeneity on the gas-phase seepage capacity was different under the lower and higher injection pressure.The macropores and micropores maybe respectively become the preferential migration pathways at the lower and higher injection pressure.In the end,the identification plate was established in our paper,and could be described the relationship among the throat heterogeneity,injection pressure,permeability and flow path of the gas phase in the tight sandstone.
基金Project(2016YFB0301104) supported by the National Key Research and Development Program of ChinaProjects(51671041,51531002) supported by the National Natural Science Foundation of ChinaProject(cstc2017jcyjBX0040) supported by the Natural Science Foundation of Chongqing City,China
基金supported by the Medical Scientific Research Foundation of Guangdong Province in China,No.B2014202the Natural Science Foundation of Guangdong Province in China,No.2014A030310455
文摘The mechanism underlying acrylamide-induced neurotoxicity remains controversial. Previous studies have focused on acrylamide-induced toxicity in adult rodents, but neurotoxicity in weaning rats has not been investigated. To explore the neurotoxic effect of acrylamide on the developing brain, weaning rats were gavaged with 0, 5, 15, and 30 mg/kg acrylamide for 4 consecutive weeks. No obvious neurotoxicity was observed in weaning rats in the low-dose acrylamide group （5 mg/kg）. However, rats from the moderateand high-dose acrylamide groups （15 and 30 mg/kg） had an abnormal gait. Furthermore, biochemical tests in these rats demonstrated that glutamate concentration was significantly reduced, and ^-aminobutyric acid content was significantly increased and was dependent on acrylamide dose. Immunohis- tochemical staining showed that in the cerebral cortex, γ-aminobutyric acid, glutamic acid decarboxylase and glial fibrillary acidic protein expression increased remarkably in the moderate- and high-dose acrylamide groups. These results indicate that in weaning rats, acrylamide is positively associated with neurotoxicity in a dose-dependent manner, which may correlate with upregulation of γ-aminobutyric acid and subsequent neuronal degeneration after the initial acrylamide exposure.
文摘Haemangiopericytoma(HPC) is a rare vascular tumor with borderline malignancy, considerable histological variability, and unpredictable clinical and biological behavior. HPC can present a diagnostic challenge because of its indeterminate clinical, radiological, and pathological features. HPC generally presents in adulthood and is equally frequent in both sexes. HPC can arise in any site in the body as a slowly growing and painless mass. The precise cell type origin of HPC is uncertain. One third of HPCs occur in the head and neck areas. Exceptional cases of hemangioblastoma arising outside the head and neck areas have been reported, but little is known about their clinicopathologic and immunohistochemical features. This study reports on a case of a large sacro-anterior HPC in a 65-year-old male.
基金Project supported by the National Natural Science Foundation of China(Grant No.U1504107)the Doctoral Scientific Research Foundation(Grant No.qd15214)
文摘Both the band filling effect and Fe/Mo disorder have a close correlation with the physical properties of the double perovskite Ca2FeMoO6. Two series of Ca2FeMoO6and Nd0.3Ca1.7FeMoO6ceramics sintered at(1050℃, 1200℃, and 1300℃) were specially designed to comparatively investigate the band-filling effect and Fe/Mo disorder on the physical properties of Ca2FeMoO6. The x-ray diffraction indicates that Fe/Mo disorder is sensitive to the sintering temperature. The magnetization behavior is mainly controlled by the Fe/Mo disorder not by the band filling effect, manifested by a close correlation of saturated magnetization(Ms) with the Fe/Mo disorder. Interestingly, magnetoresistance(MR) property of the same composition is dominantly contributed by the grain boundary strength, which can be expressed by the macroscopic resistivity values. However, the band filling effect caused by the Nd-substitution can decrease the spin polarization, and thus suppress the MR performance fundamentally. Contrary to the MR response, the Curie temperature(TC) shows an obvious optimization due to the band filling effect, which increases the carrier density near the Fermi level responsible for the ferromagnetic coupling interaction strengthen. Maybe, our work can provoke further research interests into the correlation of the band-filling effects and Fe/Mo disorder with the physical properties of other Fe/Mo-based double perovskites.
基金supported by National Natural Science Foundation of China(8147323581673453+3 种基金91129727816734868127004981373405)
文摘Pancreatic ductal adenocarcinoma(PDAC) is one of the five most malignant cancer.ZX-1201 is one of the active constituents in Alismatis Rhizoma,a well-known traditional Chinese medi.cine with a wide variety of pharmacological properties including diuretic,anti-hyperlipidemic,anti-atheroscle.rotic,anti-cancer,anti-inflammatory and anti-oxidative activities.We investigated the inhibitory effect of ZX-1201 on pancreatic cancer and the relevant molecular mechanism in vitro and in vivo.ZX-1201 inhibited the growth and metastasis of PANC-1 cells in BALB/c nude mice significantly.ZX-1201 inhibited the function of AQP1 via directly interaction and involved in the reversion process of ZX-1201 on TGF-β_1.CTGF was an important protein in the reversion process of ZX-1201 on TGF-β_1.ZX-1201 inhibited the migration of PANC-1 and CPFAC-1 cells induced by TGF-β_1 in vitro.ZX-1201 reversed the down-regu.lated of epithelial markers and up-regulated of mesenchymal markers,as well as the up-regulated of Snail and p-Smad2/3 induced by TGF-β_1.And ZX-1201 reversed Epithelial-Mesenchymal Transition by down-regulating AQP1 and inhibiting translocation of β-catenin,the promotor of CTGF.According to these,ZX-1201 inhibited the migration of pancreatic cancer cells.We concluded that ZX-1201 inhibited the growth and metastasis of PANC-1 cells in vivo significantly.And AQP1,β-catenin and CTGF were the pivotal proteins in the process of ZX-1201 inhibiting PANC-1 cells migration induced by TGF-β_1.
基金supported by National Natural Science Foundation of China(8147323581673453+3 种基金91129727816734868127004981373405)
文摘In the past two decades,with the increase of smoking population,more and more people are suffering from small cell lung cancer(SCLC).Besides,it is difficult to find an effective way to cure SCLC,since patience can easily develop drug resistance.On the other hand,with the development of science and technology,people began to study the anti-cancer strategy to increase apoptosis,such as inhibiting the overexpression of survival factors.In these survival factors,BCL-2 family has attracted a lot of attention.BH3-only protein is a member of BCL-2 family and it can directly inhibit the expression of BCL-2 protein,thereby prompting apoptosis.Since the BH3-only protein itself is difficult to become a clinical drug,to find alternatives BH3-only protein-BH3 mimetics is particularly important.Plus,more and more researchers have paid attention on the natural BH3 mimetic since it has less side-effect than artificial BH3 mimetics.To find possible BH3 mimetics,we made a primary screening with this pharma.cophore on a small molecular compounds library via Discovery Studio software.And then MTS assay were introduced to verify the activity of compounds.After that,we use Western Blot and Co-IP meth.ods to test the effect of BH3 mimetics.And finally use CDOCKER to predict the further mechanism on autophagy and apoptosis.In our studies,we found 3 possible BH3 mimetics compounds from 170,000 natural small molecular compounds via pharmacophore-based virtual screening.Furthermore,we dem.onstrated AD23,one of the 3 possible natural BH3 mimetics,induced autophagy and apoptosis simulta.neously in dose-time dependence in SCLC cell line.Finally,we use Molecular Docking to predict the further mechanism on autophagy and apoptosis.We believe our works would provide evidences and clues for the structural optimizing and further study of new drugs in the future.
基金supported by the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML2019ZD0401,GML2019ZD0407)Guangdong Basic and Applied Basic Research Foundation(2019A1515110199)+1 种基金National Natural Science Foundation of China(42006109,41825013)Key Research Program of Frontier Sciences of CAS(ZDBS-LY-DQC004)。
文摘DEAR EDITOR,The Pegasidae family,which includes seven species of seamoths,is widely distributed in the temperate and tropical waters of the Indo-Pacific,ranging from South Africa to Hawaii.In this study,we describe a new species of seamoth,Pegasus sinensis sp.nov.,based on morphological and molecular characterization of 14 specimens collected from the surrounding waters of Xiamen City,Fujian Province,China.The new species differs from closely related P.volitans in both the density and color of spots present on the dorsal and lateral body surfaces.
