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Finite element modeling of counter-roller spinning for large-sized aluminum alloy cylindrical parts 被引量:4
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作者 Dawei ZHANG Fan li +1 位作者 shuaipeng li Shengdun ZHAO 《Frontiers of Mechanical Engineering》 SCIE CSCD 2019年第3期351-357,共7页
Counter-roller spinning (CRS), where the mandrel is replaced by rollers, is an effective means of manufacturing large-sized, thin-walled, cylindrical parts with more than 2500 mm diameter. CRS is very complex because ... Counter-roller spinning (CRS), where the mandrel is replaced by rollers, is an effective means of manufacturing large-sized, thin-walled, cylindrical parts with more than 2500 mm diameter. CRS is very complex because of multi-axis rotation, multi-local loading along the circumference, and radial-axial compound deformation. Analytical or experimental methods cannot fully understand CRS. Meanwhile, numerical simulation is an adequate approach to investigate CRS with comprehensive understanding and a low cost. Thus, a finite element (FE) model of CRS was developed with the FORGE code via meshing technology, material modeling, determining the friction condition, and so on. The local fine mesh moving with the roller is one of highlights of the model. The developed 3D-FE model was validated through a CRS experiment by using a tubular blank with a 720 mm outer diameter. The developed 3D-FE model of CRS can provide a basis for parameter optimization, process control, die design, and so on. The data on force and energy predicted by the 3D-FE model can offer reasonable suggestions for determining the main mechanical parameters of CRS machines and selecting the motors. With the predicted data, an all-electric servo-drive system/machine with distributed power was designed in this work for CRS with four pairs of rollers to manufacture a large-sized, thinwalled, cylindrical part with 6000 mm diameter. 展开更多
关键词 large-sized CYLINDRICAL part counter-roller SPINNING aluminum alloy finite element method distributed power
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Ⅰ型前胶原氨基端肽化学发光免疫分析检测方法的建立及评价 被引量:1
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作者 任和 韩霜 +2 位作者 陈祥 李帅鹏 白仲虎 《生物工程学报》 CAS CSCD 北大核心 2021年第12期4406-4414,共9页
为了建立定量检测人血清中Ⅰ型前胶原氨基端肽(TypeⅠprocollagenN-terminalpeptide,PINP)的化学发光免疫分析检测方法,首先在谷氨酸棒状杆菌中分泌表达了PINP-α1链重组蛋白,以其为免疫原制备单抗,获得了2B10、8C12和1F11共3株可稳定... 为了建立定量检测人血清中Ⅰ型前胶原氨基端肽(TypeⅠprocollagenN-terminalpeptide,PINP)的化学发光免疫分析检测方法,首先在谷氨酸棒状杆菌中分泌表达了PINP-α1链重组蛋白,以其为免疫原制备单抗,获得了2B10、8C12和1F11共3株可稳定分泌抗PINP-α1链的单抗杂交瘤细胞株。进一步配对筛选后,以单抗8C12偶联生物素作为捕获抗体,单抗1F11标记辣根过氧化物酶作为检测抗体,二者与样本中的PINP结合形成夹心复合物,再与包被有链霉亲和素的磁微粒形成完整的检测系统,从而定量检测人血清中PINP的浓度。对该方法进行条件优化后,确定捕获抗体、检测抗体的最佳工作浓度均为3μg/mL,孵育时间为30 min;本方法的最低检测限为1.22 ng/mL,批内、批间的变异系数均在10%以内,线性范围为5–1 100 ng/mL,回收率在93%–107%之间。通过对160份临床样本进行检测,本方法检测结果与罗氏诊断试剂盒检测结果的相关系数R2为0.906 2。开发的磁微粒化学发光免疫分析检测方法可定量检测人血清中PINP的含量,有望成为骨骼疾病检查的辅助手段。 展开更多
关键词 磁微粒 化学发光法 Ⅰ型前胶原氨基端肽 性能评估
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