The amounts of litter produced and nutrients returned play a fundamental role in the productivity and biogeochemical and nutrient cycling of forest ecosystems.We monitored annual litterfall production, nutrient return...The amounts of litter produced and nutrients returned play a fundamental role in the productivity and biogeochemical and nutrient cycling of forest ecosystems.We monitored annual litterfall production, nutrient return,and monthly dynamics over a one-year period in Chinese fir plantations aged 10, 22, and 34 years. Our objective was to quantify litterfall and nutrient return over a complete harvest rotation of Chinese fir. Annual litterfall production increased with stand age and was recorded as(3,294.6 ± 360.4),(3,733.9 ± 211.2), and(4,876.1 ± 212.8) kg ha-1a-1in stands aged 10, 22 and 34 years, respectively. Total litter production was significantly greater in the stand aged34 years than in the stand aged 10 years(p / 0.05). With the exception of miscellaneous components, needle litterfall constituted the highest proportion(27.5–43.6 %), followed by branches/twigs(9.5–16.6 %). In all three plantations,annual total nutrient return to soil was in the order of C(1,119.95–2,709.05 kg ha-1a-1) [ N(39.32–62.04 kg ha-1a-1) [ K(15.95–22.44 kg ha-1a-1) [ P(1.30–1.63 kg ha-1a-1). C, N, K and P input to soil was significantly lower in the 10-year-old stand in comparison to the 22- and34-year-old stands(p / 0.05). Litterfall production and nutrient return(C, N and K) followed similar patterns, and C and N input to soil was significantly related to litterfall production(needle, branch and total litterfall). C, N, P and K input to soil and total litterfall production were mainly driven by needle litterfall.展开更多
Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as"autotrophic cell factories"for biosynthesis of various biofuels directly from CO_(2).However,the low tolerance to...Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as"autotrophic cell factories"for biosynthesis of various biofuels directly from CO_(2).However,the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts.In this study,RNAseq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp.PCC 6803.Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly.To further identify regulatory genes related to n-hexane tolerance,a library of transcriptional regulators(TRs)deletion mutants was then screened for their roles in nhexane tolerance.The results showed that a knockout mutant of slr0724 that encodes an Hta R suppressor protein was more tolerant to n-hexane than the wild type,indicating the involvement of slr0724 in nhexane tolerance.This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp.PCC 6803,which could contribute to the further engineering of nhexane tolerance in cyanobacteria.展开更多
Mutants lacking wild-type MLO(Mildew resistance Locus O)proteins show broad-spectrum resistance to the powdery mildew fungus,and dysregulated cell death control,with spontaneous cell death in response to developmental...Mutants lacking wild-type MLO(Mildew resistance Locus O)proteins show broad-spectrum resistance to the powdery mildew fungus,and dysregulated cell death control,with spontaneous cell death in response to developmental or abiotic stimuli.In order to understand the evolution and divergence patterns of the MLO gene family in Rosaceae plants,we analysed systematically genome-wide data from Fragaria vesca,Prunus persica,Prunus mume,Malus domestica,Pyrus bretschneideri and Rubus occidentalis based on bioinformatics methods.Using three phylogenetic methods(the neighbour-joining,maximum likelihood,and Bayesian methods),we identified 117 MLO genes from 6 Rosaceae species.The results of all three phylogenetic analysis methods supported that these genes were divided into six clades.Conserved motif analysis found that only motif 2 was present in all MLO proteins and had 3 nearly invariant amino acid residues.The findings indicated that motif 2 might be shared by the MLO gene family.The structural features of these genes showed large variations in sequence length among different species,although the lengths and the numbers of exons exhibited high degrees of similarity.Selective pressure analysis showed extremely significant differences in all 6 clades,with 2,1,and 1 site(s)under significant positive selection detected in clades III,IV,and VI,respectively.These positive selection sites were important driving forces for the promotion of the functional differentiation of the MLO genes.Functional divergence analysis showed that the significantly divergent sites were located within the domains of the MLO genes.Functional distance analysis showed that the clade V had more conservative functions and might have retained more original functions during the evolutionary process.However,clade I may have undergone extensive altered functional constraints as a specialised functional role.Moreover,the most original function of the MLO genes in Rosaceae could be related to the evolution of their resistance to powdery mildew,which then gradually evolved into functions such as the regulation of flower development,the control of root morphology,and seed evolution due to the different evolutionary rates after gene duplication.These results provide a theoretical basis for further studies of the molecular evolutionary patterns of the plant MLO gene family.展开更多
Based on decreasing the flexibility of the power grid through the integration of large-scale renewable energy,a multi-energy storage system architectural model and its coor-dination operational strategy with the same ...Based on decreasing the flexibility of the power grid through the integration of large-scale renewable energy,a multi-energy storage system architectural model and its coor-dination operational strategy with the same flexibility as in the pumped storage power station and battery energy storage system(BESS)are studied.According to the new energy fluctuation characteristics and the different peak valley parameters in the power grid,this paper proposes a electricity heat hydrogen multi-energy storage system(EHH-MESS)and its coordination and optimization operational model to reduce the curtailment of wind power and photovoltaic(PV)to the power grid and improve the flexibility of the power grid.Finally,this paper studied the simulation model of an energy storage optimization control strategy after the multi-energy storage system is connected to the distribution networks,and analyzed three operational modes of the multi-energy storage system.The simulation results show that the EHH-MESS proposed in this paper has a better power grid regulation flexibility and economy,and can be used to replace the battery energy storage system based on MATLAB.展开更多
Dyestuffs and heavy metal ions in water are seriously harmful to the ecological environment and human health. Three-dimensional (3D) flowerlike Fe(OH)3 microspheres were synthesized through a green yet low-cost in...Dyestuffs and heavy metal ions in water are seriously harmful to the ecological environment and human health. Three-dimensional (3D) flowerlike Fe(OH)3 microspheres were synthesized through a green yet low-cost injection method, for the removal of organic dyes and heavy metal ions. The Fe(OH)3 microspheres were characterized by thermal gravimetric analysis (TGA), Fourier transform infrared (FT-IR}, and transmission electron microscopy (TEM) techniques. The adsorption kinetics of Congo Red (CR) on Fe(OH)3 microspheres obeyed the pseudo-second-order model. Cr6+ and Pb2+ adsorption behaviors on Fe(OH)3 microspheres followed the Langmuir isotherm model. The maximum adsorption capacities of the synthesized Fe(OH)3 were 308, 52.94, and 75.64 mg/g for CR, Cr6+, and Pb2+ respectively. The enhanced adsorption performance originated from its surface properties and large specific surface area of 250 m2/g. The microspheres also have excellent adsorption stability and recyclability. Another merit of the Fe(OH)3 material is that it also acts as a Fenton-like catalyst. These twin functionalities (both as adsorbent and Fenton-like catalyst) give the synthesized Fe(OH)3 microspheres great potential in the field of water treatment.展开更多
基金supported by National Natural Science Foundation of China(Grant Nos.31370619 and 31100472)Doctoral Program of Higher Education of Ministry of Education of China(Grant No.20113515110009)+2 种基金Forestry Public Benefit Research Projects of National Forestry Administration(Grant No.201304303)Science and Technology Major Project of the Fujian Province(Grant No.2012NZ0001–1)Training Program Foundation for University Distinguished Young Talents of Fujian Province,P.R.China(Grant No.JA 12091)
文摘The amounts of litter produced and nutrients returned play a fundamental role in the productivity and biogeochemical and nutrient cycling of forest ecosystems.We monitored annual litterfall production, nutrient return,and monthly dynamics over a one-year period in Chinese fir plantations aged 10, 22, and 34 years. Our objective was to quantify litterfall and nutrient return over a complete harvest rotation of Chinese fir. Annual litterfall production increased with stand age and was recorded as(3,294.6 ± 360.4),(3,733.9 ± 211.2), and(4,876.1 ± 212.8) kg ha-1a-1in stands aged 10, 22 and 34 years, respectively. Total litter production was significantly greater in the stand aged34 years than in the stand aged 10 years(p / 0.05). With the exception of miscellaneous components, needle litterfall constituted the highest proportion(27.5–43.6 %), followed by branches/twigs(9.5–16.6 %). In all three plantations,annual total nutrient return to soil was in the order of C(1,119.95–2,709.05 kg ha-1a-1) [ N(39.32–62.04 kg ha-1a-1) [ K(15.95–22.44 kg ha-1a-1) [ P(1.30–1.63 kg ha-1a-1). C, N, K and P input to soil was significantly lower in the 10-year-old stand in comparison to the 22- and34-year-old stands(p / 0.05). Litterfall production and nutrient return(C, N and K) followed similar patterns, and C and N input to soil was significantly related to litterfall production(needle, branch and total litterfall). C, N, P and K input to soil and total litterfall production were mainly driven by needle litterfall.
基金supported by grants from the National Key Research and Development Program of China(2020YFA0906800,2021YFA0909700,2018YFA0903600 and 2019YFA0904600)。
文摘Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as"autotrophic cell factories"for biosynthesis of various biofuels directly from CO_(2).However,the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts.In this study,RNAseq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp.PCC 6803.Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly.To further identify regulatory genes related to n-hexane tolerance,a library of transcriptional regulators(TRs)deletion mutants was then screened for their roles in nhexane tolerance.The results showed that a knockout mutant of slr0724 that encodes an Hta R suppressor protein was more tolerant to n-hexane than the wild type,indicating the involvement of slr0724 in nhexane tolerance.This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp.PCC 6803,which could contribute to the further engineering of nhexane tolerance in cyanobacteria.
基金supported by the National Key R&D Program of China(Grant No.2018YFD1000400)National Natural Science Foundation of China(Grant Nos.31860571 and 31560565)+1 种基金Major Science and Technology Projects Yunnan Province(Grant No.2016ZA005)Yunnan Youth Academic&Technical Leaders Reserve Talents Training Project(Grant No.2015HB078)。
文摘Mutants lacking wild-type MLO(Mildew resistance Locus O)proteins show broad-spectrum resistance to the powdery mildew fungus,and dysregulated cell death control,with spontaneous cell death in response to developmental or abiotic stimuli.In order to understand the evolution and divergence patterns of the MLO gene family in Rosaceae plants,we analysed systematically genome-wide data from Fragaria vesca,Prunus persica,Prunus mume,Malus domestica,Pyrus bretschneideri and Rubus occidentalis based on bioinformatics methods.Using three phylogenetic methods(the neighbour-joining,maximum likelihood,and Bayesian methods),we identified 117 MLO genes from 6 Rosaceae species.The results of all three phylogenetic analysis methods supported that these genes were divided into six clades.Conserved motif analysis found that only motif 2 was present in all MLO proteins and had 3 nearly invariant amino acid residues.The findings indicated that motif 2 might be shared by the MLO gene family.The structural features of these genes showed large variations in sequence length among different species,although the lengths and the numbers of exons exhibited high degrees of similarity.Selective pressure analysis showed extremely significant differences in all 6 clades,with 2,1,and 1 site(s)under significant positive selection detected in clades III,IV,and VI,respectively.These positive selection sites were important driving forces for the promotion of the functional differentiation of the MLO genes.Functional divergence analysis showed that the significantly divergent sites were located within the domains of the MLO genes.Functional distance analysis showed that the clade V had more conservative functions and might have retained more original functions during the evolutionary process.However,clade I may have undergone extensive altered functional constraints as a specialised functional role.Moreover,the most original function of the MLO genes in Rosaceae could be related to the evolution of their resistance to powdery mildew,which then gradually evolved into functions such as the regulation of flower development,the control of root morphology,and seed evolution due to the different evolutionary rates after gene duplication.These results provide a theoretical basis for further studies of the molecular evolutionary patterns of the plant MLO gene family.
基金This project was supported by National Key Research and Development Plan(2017YFB0902100).
文摘Based on decreasing the flexibility of the power grid through the integration of large-scale renewable energy,a multi-energy storage system architectural model and its coor-dination operational strategy with the same flexibility as in the pumped storage power station and battery energy storage system(BESS)are studied.According to the new energy fluctuation characteristics and the different peak valley parameters in the power grid,this paper proposes a electricity heat hydrogen multi-energy storage system(EHH-MESS)and its coordination and optimization operational model to reduce the curtailment of wind power and photovoltaic(PV)to the power grid and improve the flexibility of the power grid.Finally,this paper studied the simulation model of an energy storage optimization control strategy after the multi-energy storage system is connected to the distribution networks,and analyzed three operational modes of the multi-energy storage system.The simulation results show that the EHH-MESS proposed in this paper has a better power grid regulation flexibility and economy,and can be used to replace the battery energy storage system based on MATLAB.
基金supported by the National Key Research and Development Programme(No.2016YFC0401104)Harbin Institute of Technology(HIT) Environment and Ecology Innovation Special Funds(No.HSCJ201617)+1 种基金the National Natural Science Foundation of China(Nos.21773050,21528501)State Key Laboratory of Urban Water Resource and Environment(Harbin Institute of Technology)(No.2017DX05)
文摘Dyestuffs and heavy metal ions in water are seriously harmful to the ecological environment and human health. Three-dimensional (3D) flowerlike Fe(OH)3 microspheres were synthesized through a green yet low-cost injection method, for the removal of organic dyes and heavy metal ions. The Fe(OH)3 microspheres were characterized by thermal gravimetric analysis (TGA), Fourier transform infrared (FT-IR}, and transmission electron microscopy (TEM) techniques. The adsorption kinetics of Congo Red (CR) on Fe(OH)3 microspheres obeyed the pseudo-second-order model. Cr6+ and Pb2+ adsorption behaviors on Fe(OH)3 microspheres followed the Langmuir isotherm model. The maximum adsorption capacities of the synthesized Fe(OH)3 were 308, 52.94, and 75.64 mg/g for CR, Cr6+, and Pb2+ respectively. The enhanced adsorption performance originated from its surface properties and large specific surface area of 250 m2/g. The microspheres also have excellent adsorption stability and recyclability. Another merit of the Fe(OH)3 material is that it also acts as a Fenton-like catalyst. These twin functionalities (both as adsorbent and Fenton-like catalyst) give the synthesized Fe(OH)3 microspheres great potential in the field of water treatment.