Gastrodia elata (G. elata ) is a traditional Chinese herbal medicine. In recent years, G. elata has been widely used in food and pharmaceutical industries because of its excellent health benefits. However, the stabili...Gastrodia elata (G. elata ) is a traditional Chinese herbal medicine. In recent years, G. elata has been widely used in food and pharmaceutical industries because of its excellent health benefits. However, the stability and bioactivity of phenolic compounds from G. elata after in vitro gastrointestinal digestion remain unknown. In this study, the aqueous extract of G. elata rhizomes was used to simulate gastrointestinal digestion and colonic fermentation in vitro . This study aimed to evaluate the bioaccessibility during the simulation of gastrointestinal and colonic fermentation and to clarify the effects of different digestion stages on the anti-oxidant and enzyme inhibitory activities and intestinal health of samples in vitro . The results indicated that the total polyphenol and flavonoid contents after colonic fermentation for 72 h were 5.48- and 3.24- fold higher than those without digestion, respectively. Except for gastrodigenin and ferulic acid, the contents and bioaccessibility of the other phenols after fermentation were higher than those in the small-intestine stage. Antioxidant activity was positively correlated with the total polyphenol content. The inhibitory activities of pancrelipase and cholinesterase were enhanced by gastric digestion and colonic fermentation. The sample promoted the production of Lactobacillus and decreased the abundance of pathogenic bacteria. Moreover, the content of short-chain fatty acids also exhibited an upward trend. Therefore, G. elata has the potential to develop into a health food with anti-oxidant, anti-obesity, and intestinal health functions.展开更多
基金supported by the Shaanxi Province Key Research and Development Plan[2020NY-139]Scientific Research Program Funded by Shaanxi Provincial Education Department[19JC012]the Scientific Research Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas(Joint construction of Hanzhong City&Shaanxi University of Technology).
文摘Gastrodia elata (G. elata ) is a traditional Chinese herbal medicine. In recent years, G. elata has been widely used in food and pharmaceutical industries because of its excellent health benefits. However, the stability and bioactivity of phenolic compounds from G. elata after in vitro gastrointestinal digestion remain unknown. In this study, the aqueous extract of G. elata rhizomes was used to simulate gastrointestinal digestion and colonic fermentation in vitro . This study aimed to evaluate the bioaccessibility during the simulation of gastrointestinal and colonic fermentation and to clarify the effects of different digestion stages on the anti-oxidant and enzyme inhibitory activities and intestinal health of samples in vitro . The results indicated that the total polyphenol and flavonoid contents after colonic fermentation for 72 h were 5.48- and 3.24- fold higher than those without digestion, respectively. Except for gastrodigenin and ferulic acid, the contents and bioaccessibility of the other phenols after fermentation were higher than those in the small-intestine stage. Antioxidant activity was positively correlated with the total polyphenol content. The inhibitory activities of pancrelipase and cholinesterase were enhanced by gastric digestion and colonic fermentation. The sample promoted the production of Lactobacillus and decreased the abundance of pathogenic bacteria. Moreover, the content of short-chain fatty acids also exhibited an upward trend. Therefore, G. elata has the potential to develop into a health food with anti-oxidant, anti-obesity, and intestinal health functions.
