Bumblebees are a genus of pollinators(Bombus)that play important roles in natural ecosystem and agricultural production.Several bumblebee species have been recorded as under population decline,and the proportion of sp...Bumblebees are a genus of pollinators(Bombus)that play important roles in natural ecosystem and agricultural production.Several bumblebee species have been recorded as under population decline,and the proportion of species experiencing popula-tion decline within subgenus Thoracobombus is higher than average.Bombus opulentus is 1 species in Thoracobombus,but little is known about its recent population dynamics.Here,we employed conservation genomics methods to investigate the population dynam-ics of B.opulentus during the recent past and identify the likely environmental factors that may cause population decline.Firstly,we placed the scaffold-level of B.opulentus ref-erence genome sequence onto chromosome-level using Hi-C technique.Then,based on this reference genome and whole-genome resequencing data for 51 B.opulentus samples,we reconstructed the population structure and effective population size(Ne)trajectories of B.opulentus and identified genes that were under positive selection.Our results revealed that the collected B.opulentus samples could be divided into 2 populations,and 1 of them experienced a recent population decline;the declining population also exhibited lower ge-netic diversity and higher inbreeding levels.Genes related to high-temperature tolerance,immune response,and detoxication showed signals of positive selection in the declining population,suggesting that climate warming and pathogen/pesticide exposures may con-tribute to the decline of this B.opulentus population.Taken together,our study provided insights into the demography of B.opulentus populations and highlighted that popula-tions of the same bumblebee species could have contrasting Ne trajectories and population decline could be caused by a combination of various stressors.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant numbers 32270445 and 31971397)the Support Project of High-level Teachers in Beijing Municipal Universities in the Period of 14th Fiveyear Plan(Grant number BPHR20220114)the Central Public-interest Scientific Institution Basal Research Fund(Grant numbers Y2019XK13 and Y2021XK16).
文摘Bumblebees are a genus of pollinators(Bombus)that play important roles in natural ecosystem and agricultural production.Several bumblebee species have been recorded as under population decline,and the proportion of species experiencing popula-tion decline within subgenus Thoracobombus is higher than average.Bombus opulentus is 1 species in Thoracobombus,but little is known about its recent population dynamics.Here,we employed conservation genomics methods to investigate the population dynam-ics of B.opulentus during the recent past and identify the likely environmental factors that may cause population decline.Firstly,we placed the scaffold-level of B.opulentus ref-erence genome sequence onto chromosome-level using Hi-C technique.Then,based on this reference genome and whole-genome resequencing data for 51 B.opulentus samples,we reconstructed the population structure and effective population size(Ne)trajectories of B.opulentus and identified genes that were under positive selection.Our results revealed that the collected B.opulentus samples could be divided into 2 populations,and 1 of them experienced a recent population decline;the declining population also exhibited lower ge-netic diversity and higher inbreeding levels.Genes related to high-temperature tolerance,immune response,and detoxication showed signals of positive selection in the declining population,suggesting that climate warming and pathogen/pesticide exposures may con-tribute to the decline of this B.opulentus population.Taken together,our study provided insights into the demography of B.opulentus populations and highlighted that popula-tions of the same bumblebee species could have contrasting Ne trajectories and population decline could be caused by a combination of various stressors.
