Osteoimmunology research in rheumatoid arthritis:From single-cell omics approach

Cellular immune responses as well as generalized and periarticular bone loss are the key pathogenic features of rheumatoid arthritis(RA).Under the pathological conditions of RA,dysregulated inflammation and immune processes tightly interact with skeletal system,resulting in pathological bone damage via inhibition of bone formation or induction of bone resorption.Singlecell omics technologies are revolutionary tools in the field of modern biological research.They enable the display of the state and function of cells in various environments from a single-cell resolution,thus making it conducive to identify the dysregulated molecular mechanisms of bone destruction in RA as well as the discovery of potential therapeutic targets and biomarkers.Here,we summarize the latest findings of single-cell omics technologies in osteoimmunology research in RA.These results suggest that single-cell omics have made significant contributions to transcriptomics and dynamics of specific cells involved in bone remodeling,providing a new direction for our understanding of cellular heterogeneity in the study of osteoimmunology in RA.

Recent advancements and manipulation strategies of colloidal Cs_(2)B^(I)B^(III)X_(6)lead-free halide double perovskite nanocrystals

Double-metallic lead-free halide perovskites,Cs_(2)B^(I)B^(III)X_(6),sharing three-dimensional crystal structure,have been under the spotlight as the promising alternatives for the toxic and instable lead-based counterparts.Interest in Cs_(2)B^(I)B^(III)X_(6)motivates intense research into their colloidal nanocrystals(NCs).Recently,Cs_(2)B^(I)B^(III)X_(6)NCs have made great progress in the optical performance via alloying or doping,but there are still great challenges for optoelectronic applications.In this review,the latest advances of Cs_(2)B^(I)B^(III)X_(6)NCs in synthesis approaches,bandgap engineering,photoluminescence(PL)optimization,and applications are summarized.The focus is put upon the composition-property relationships of Cs_(2)B^(I)B^(III)X_(6)NCs,which is approached by discussing the influences of composition variation on the electronic states,carrier dynamics,and optical properties.The challenges and the corresponding improving strategies in the development of high-effective and stable Cs_(2)B^(I)B^(III)X_(6)NCs for device applications are also highlighted.It is believed that this review can deepen the understanding on this burgeoning material system and shed light on their future research directions.

Effects of alfalfa coverage on runoff,erosion and hydraulic characteristics of overland flow on loess slope plots

An evaluation of the interactions between vegetation,overland and soil erosion can provide valuable insight for the conservation of soil and water.An experiment was conducted to study water infiltration,runoff generation process,rate of sediment erosion,and hydrodynamic characteristics of overland flow from a sloping hillside with different draw-off discharges from alfalfa and control plots with 20°slope.The effect of alfalfa on runoff and sediment transport reduction was quantitatively analyzed.Alfalfa was discussed for its ability to reduce the overland flow scouring force or change the runoff movement.Compared to the bare-soil plots,alfalfa plots generated a 1.77 times increase in infiltration rate.Furthermore,the down-slope water infiltration rate for the bare soil plots was higher than in the up-slope,while the opposite was found in the alfalfa plots.In addition,alfalfa had a significant effect on runoff and sediment yield.In comparison to the control,the runoff coefficient and sediment transportation rate decreased by 28.3%and 78.4%in the grass slope,respectively.The runoff generated from the alfalfa and bare-soil plots had similar trends with an initial increase and subsequent leveling to a steady-state rate.The transport of sediment reduced with time as a consequence of the depletion of loose surface materials.The maximum sediment concentration was recorded within the first few minutes of each event.The alfalfa plots had subcritical flow while the baresoil plots had supercritical flow,which indicate that the capability of the alfalfa slope for resisting soil erosion and sediment movement was greater than for bare soil plots.Moreover,the flow resistance coefficient and roughness coefficient for the alfalfa plots were both higher than for the bare-soil plots,which indicate that overland flow in alfalfa plots had retarded and was blocked,and the flow energy along the runoff path had gradually dissipated.Finally,the ability to erode and transport sediment had decreased.

Ephemeral gully recognition and accuracy evaluation using deep learning in the hilly and gully region of the Loess Plateau in China

Ephemeral gullies are widely distributed in the hilly and gully region of the Loess Plateau and play a unique role in the slope gully erosion system.Rapid and accurate identification of ephemeral gullies impacts the distribution law and development trend of soil erosion on the Loess Plateau.Deep learning algorithms can quickly and accurately process large data samples that recognize ephemeral gullies from remote sensing images.Here,we investigated ephemeral gullies in the Zhoutungou watershed in the hilly and gully region of the Loess Plateau in China using satellite and unmanned aerial vehicle images and combined a deep learning image semantic segmentation model to realize automatic recognition and feature extraction.Using Accuracy,Precision,Recall,F1value,and AUC,we compared the ephemeral gully recognition results and accuracy evaluation of U-Net,R2U-Net,and SegNet image semantic segmentation models.The SegNet model was ranked first,followed by the R2U-Net and U-Net models,for ephemeral gully recognition in the hilly and gully region of the Loess Plateau.The ephemeral gully length and width between predicted and measured values had RMSE values of 6.78 m and 0.50 m,respectively,indicating that the model has an excellent recognition effect.This study identified a fast and accurate method for ephemeral gully recognition in the hilly and gully region of the Loess Plateau based on remote sensing images to provide an academic reference and practical guidance for soil erosion monitoring and slope and gully management in the Loess Plateau region.

Lewis acid-catalyzed[4+2]cycloaddition of 3-alkyl-2-vinylindoles withβ,γ-unsaturatedα-ketoesters

A Lewis acid-catalyzed[4+2]cycloaddition of 3-alkyl-2-vinylindoles withβ,γ-unsaturatedα-ketoesters has been established in the presence of Sc(OTf)3,which afforded a series of indole-containing pyran derivatives in generally good yields(up to 90%yield)with excellent diastereoselectivities(up to >95:5 dr)under mild conditions.This approach not only enriches the chemistry of 3-alkyl-2-vinylindoles,but also has provided an atom-economic method for the synthesis of indole-containing pyran derivatives with potential bioactivity.