Purpose:To observe the dynamic changes of hydration in galactose induced cataract.Methods:Two groups of wistar rats were used in the experiment.There were 12rats in the experimental group,which were fed diet of 50%D-G...Purpose:To observe the dynamic changes of hydration in galactose induced cataract.Methods:Two groups of wistar rats were used in the experiment.There were 12rats in the experimental group,which were fed diet of 50%D-Galactose standard feed;while the control group had8rats fed standard feed.Their other living conditions were the same,At desired time periods,two Wistar rats fed galactose and one normal control were selected and Killed20minutes before the instrument examination respectively,them,their lenses were repoved from the orbs by a posterior approach.The cleaned fresh lans was placed in a quartz cuvette with Tris buffered balanced salt solution containing5.5mmol/L glucose.The quartz cuvette was placed on the stage of the Spectroneter.The laser beam was focused at the lens nuclear fromthe bottom of the cuvette and the scattered light was col-lected at 90°to the incident beam.Results:Raman spectroscopy showed that(1)during the formation of galactose cataract,the Water signal(at3390cm-1)increased obiviously,and the ratio of I3390/I2935increased from0.31(3days)to2.26(17days).which is correlated with the imbibition of wter in the lens nuclear;（2）the hydration of lens nuclear could be divided into two phases.The ratio I3390/I2935was increased slowly and steadily by 11days after galactose feeding,Then,the ratio tured to increase quite fast till17days.Conclusion:The hydration of nuclear is changed simultaneously with the forman-tion of cataract,The hydration of nuclear is mainly due to the impalance of Na^+/K^+.Eye Science1995;11:143-146.展开更多
文摘Purpose:To observe the dynamic changes of hydration in galactose induced cataract.Methods:Two groups of wistar rats were used in the experiment.There were 12rats in the experimental group,which were fed diet of 50%D-Galactose standard feed;while the control group had8rats fed standard feed.Their other living conditions were the same,At desired time periods,two Wistar rats fed galactose and one normal control were selected and Killed20minutes before the instrument examination respectively,them,their lenses were repoved from the orbs by a posterior approach.The cleaned fresh lans was placed in a quartz cuvette with Tris buffered balanced salt solution containing5.5mmol/L glucose.The quartz cuvette was placed on the stage of the Spectroneter.The laser beam was focused at the lens nuclear fromthe bottom of the cuvette and the scattered light was col-lected at 90°to the incident beam.Results:Raman spectroscopy showed that(1)during the formation of galactose cataract,the Water signal(at3390cm-1)increased obiviously,and the ratio of I3390/I2935increased from0.31(3days)to2.26(17days).which is correlated with the imbibition of wter in the lens nuclear;（2）the hydration of lens nuclear could be divided into two phases.The ratio I3390/I2935was increased slowly and steadily by 11days after galactose feeding,Then,the ratio tured to increase quite fast till17days.Conclusion:The hydration of nuclear is changed simultaneously with the forman-tion of cataract,The hydration of nuclear is mainly due to the impalance of Na^+/K^+.Eye Science1995;11:143-146.
