This paper describes a pneumatic balloon actuator(PBA)composed of polydimethylsiloxane(PDMS)for cellular aggregate manipulation.We evaluated the ability of the microdevice to manipulate a tiny and sensitive cellular a...This paper describes a pneumatic balloon actuator(PBA)composed of polydimethylsiloxane(PDMS)for cellular aggregate manipulation.We evaluated the ability of the microdevice to manipulate a tiny and sensitive cellular aggregate without causing serious damage.We used human mesenchymal stem cells(hMSCs)for the cellular aggregate.We describe the design,fabrication,characterization and operation of the soft microfingers to pinch and release a spherical hMSC aggregate(φ200μm),and we employed a PBA to serve as an artificial muscle to drive the microfingers.A design of the microfingers in terms of dimensions,generated force and contact conditions was accomplished.The designed dimensions of a single finger were 560μm×900μm.In summary,we demonstrate the utility of the surface modification of a fingertip for pinching and releasing a cellular aggregate and describe a manipulation system that was constructed to drive and control the microfingers.The implemented manipulation system,which is composed of microfingers and a positioning mechanism,was tested and verified in a series of operations.展开更多
基金This work was supported by Grant-in-Aid for Scientific Research(A):JSPS KAKENHI Grant Number 24240075.
文摘This paper describes a pneumatic balloon actuator(PBA)composed of polydimethylsiloxane(PDMS)for cellular aggregate manipulation.We evaluated the ability of the microdevice to manipulate a tiny and sensitive cellular aggregate without causing serious damage.We used human mesenchymal stem cells(hMSCs)for the cellular aggregate.We describe the design,fabrication,characterization and operation of the soft microfingers to pinch and release a spherical hMSC aggregate(φ200μm),and we employed a PBA to serve as an artificial muscle to drive the microfingers.A design of the microfingers in terms of dimensions,generated force and contact conditions was accomplished.The designed dimensions of a single finger were 560μm×900μm.In summary,we demonstrate the utility of the surface modification of a fingertip for pinching and releasing a cellular aggregate and describe a manipulation system that was constructed to drive and control the microfingers.The implemented manipulation system,which is composed of microfingers and a positioning mechanism,was tested and verified in a series of operations.
