Today’s chrysanthemums are highly evolved flowering plants and they are considered as one of the most important ornamental cut flowers. In this research an isopentenyl transferase gene named CmIPT1 was isolated from ...Today’s chrysanthemums are highly evolved flowering plants and they are considered as one of the most important ornamental cut flowers. In this research an isopentenyl transferase gene named CmIPT1 was isolated from Chrysanthemum morifolium cv. ‘Jinba’ using RACE and RT-PCR methods. The full cDNA sequence of CmIPT1 was 873 bp which encoded a deduced protein of 290 amino acids. It contained GxxGxGKS which is a conserved sequence of the typical domain of IPT family. The phylogenetic tree analysis of CmIPT1 in Chrysanthemum morifolium cv. ‘Jinba’ shows that it has the closest relationship with CcIPT1 in Cynara cardunculus var. scolymus. Expression of CmIPT1 was higher in stems and apex, whereas it was lower in leaves and roots. And the overexpression of CmIPT1 obviously increased the number of rosette branches in Arabidopsis. Here, in our study, we showed that CmIPT1 is a positive regulator of branch development in Chrysanthemum and may play a key role in regulating lateral branch formation of Chrysanthemum plants.展开更多
Decreasing the hardness of pomegranate seeds by reducing the content of lignin is an effective way to develop soft-seeded pomegranate. Laccases (LAC) is a key regulatory factor in lignin synthesis. The full-length seq...Decreasing the hardness of pomegranate seeds by reducing the content of lignin is an effective way to develop soft-seeded pomegranate. Laccases (LAC) is a key regulatory factor in lignin synthesis. The full-length sequence of PgLAC was obtained from “Punica granatum cv. Hongyushizi”, by using RACE and RT-PCR methods. PgLAC had an open reading frame of 1716 bp and encoded a protein of 571 amino acids. Phylogenetic tree analysis showed that PgLAC was most closely related to the LAC5 ortholog identified in Eucalyptus grandis (EgLAC5). Expression analysis showed that expression of PgLAC was higher in “Hongyushizi”, while lower in “Huiliruanzi” and “Tunisiruanzi”;PgLAC was predominantly expressed in stems;From 20 to 80 days after full bloom, the expression of PgLAC increased and reached a maximum at 80 d, then gradually decreased. These results suggested that PgLAC may be a candidate gene for reducing the hardness of pomegranate seeds.展开更多
文摘Today’s chrysanthemums are highly evolved flowering plants and they are considered as one of the most important ornamental cut flowers. In this research an isopentenyl transferase gene named CmIPT1 was isolated from Chrysanthemum morifolium cv. ‘Jinba’ using RACE and RT-PCR methods. The full cDNA sequence of CmIPT1 was 873 bp which encoded a deduced protein of 290 amino acids. It contained GxxGxGKS which is a conserved sequence of the typical domain of IPT family. The phylogenetic tree analysis of CmIPT1 in Chrysanthemum morifolium cv. ‘Jinba’ shows that it has the closest relationship with CcIPT1 in Cynara cardunculus var. scolymus. Expression of CmIPT1 was higher in stems and apex, whereas it was lower in leaves and roots. And the overexpression of CmIPT1 obviously increased the number of rosette branches in Arabidopsis. Here, in our study, we showed that CmIPT1 is a positive regulator of branch development in Chrysanthemum and may play a key role in regulating lateral branch formation of Chrysanthemum plants.
文摘Decreasing the hardness of pomegranate seeds by reducing the content of lignin is an effective way to develop soft-seeded pomegranate. Laccases (LAC) is a key regulatory factor in lignin synthesis. The full-length sequence of PgLAC was obtained from “Punica granatum cv. Hongyushizi”, by using RACE and RT-PCR methods. PgLAC had an open reading frame of 1716 bp and encoded a protein of 571 amino acids. Phylogenetic tree analysis showed that PgLAC was most closely related to the LAC5 ortholog identified in Eucalyptus grandis (EgLAC5). Expression analysis showed that expression of PgLAC was higher in “Hongyushizi”, while lower in “Huiliruanzi” and “Tunisiruanzi”;PgLAC was predominantly expressed in stems;From 20 to 80 days after full bloom, the expression of PgLAC increased and reached a maximum at 80 d, then gradually decreased. These results suggested that PgLAC may be a candidate gene for reducing the hardness of pomegranate seeds.
