[ Objective] This study aimed to establish an electrochemiluminescence method for determining the trace molybdenum in plant seeds. [ Method ] The equipment used in this study consisted of an electrogenerated reagent s...[ Objective] This study aimed to establish an electrochemiluminescence method for determining the trace molybdenum in plant seeds. [ Method ] The equipment used in this study consisted of an electrogenerated reagent system and a flow-injection chemiluminescence detection system. The optimal electric poten- tial, reagent acidity, flow rate of sample solution, as well as the concentration and alkalinity of luminal were screened to measure the trace molybdenum in plant seeds. [ Result I The unstable molybdenum （III） was on-line electrogenerated from molybdenum （VI） via controlled potential electrolysis technique using a home- made flow-through carbon electrolytic cell at the potential of -0.60 V （vs Ag/AgC1）. The method allowed the determination of 5.0 × 10-10 _ 5.0 ×10-7 g/ml mo- lybdenum with a detection limit of 5 × 10 u g/ml molybdenum （VI）. The relative standard deviation was 2.6% for the 1.0 × 10 -9 g/ml molybdenum solution in 11 measurements. [ Conclusion] Compared with the previous methods, this electrochemilum^nescence method not only improved the selectivity of the determina- tion, but also avoided the use of toxic reagent mercury.展开更多
基金Supported by the National Natural Science Foundation of China(20275023)
文摘[ Objective] This study aimed to establish an electrochemiluminescence method for determining the trace molybdenum in plant seeds. [ Method ] The equipment used in this study consisted of an electrogenerated reagent system and a flow-injection chemiluminescence detection system. The optimal electric poten- tial, reagent acidity, flow rate of sample solution, as well as the concentration and alkalinity of luminal were screened to measure the trace molybdenum in plant seeds. [ Result I The unstable molybdenum （III） was on-line electrogenerated from molybdenum （VI） via controlled potential electrolysis technique using a home- made flow-through carbon electrolytic cell at the potential of -0.60 V （vs Ag/AgC1）. The method allowed the determination of 5.0 × 10-10 _ 5.0 ×10-7 g/ml mo- lybdenum with a detection limit of 5 × 10 u g/ml molybdenum （VI）. The relative standard deviation was 2.6% for the 1.0 × 10 -9 g/ml molybdenum solution in 11 measurements. [ Conclusion] Compared with the previous methods, this electrochemilum^nescence method not only improved the selectivity of the determina- tion, but also avoided the use of toxic reagent mercury.
