Drought causes water shortage and consequent retardation of plants growth and development.Therefore,improving the drought tolerance of plants is necessary for expanding cultivation and resource promotion.Increasing ev...Drought causes water shortage and consequent retardation of plants growth and development.Therefore,improving the drought tolerance of plants is necessary for expanding cultivation and resource promotion.Increasing evidence indicates that phospholipase is involved in the response of plants to drought stress.The objective of this study was to create new drought-tolerant chrysanthemum germplasm,which lays a foundation for the study of the molecular mechanism of phospholipase mediated stress response in chrysanthemum.CmPLDαhas the closest relationship with sunflower HaPLDα,and belongs to the PLDαfamily.CmPLDαover-expressing plants showed a slight shrinking under 20%PEG6000 treatment.The survival rate increased significantly by 1.7−1.8 times that of the wild type.Relative water content(RWC)of CmPLDαover-expressing plants were nearly 10%higher than that of the wild type.Relative electrical conductivity and MDA content were significantly lower than those of the wild type.ABA content of the over-expression lines Z1,Z2 were 1.3 and 1.22 times that of wild type,but ABA content of antisense lines F1,F2 was approximately 0.83 and 0.81 of those of wild type.Most plants of antisense transgenic lines F1,F2 were wrinkled,with a wilting index of 5 and 6,and the survival rate was also lower than that of the wild type after recovery growth.RWC of antisense lines were lower than over-expression lines,relative electrical conductivity and MDA content were significantly higher than those of the wild type.In summary,CmPLDαcould enhance tolerance of chrysanthemum to drought conditions.展开更多
基金funded by the National Key Research and Development Program of China(2018YFD1000402)the National Natural Science Foundation of China(32030098)Jiangsu Agriculture Science and Technology Innovation Fund(CX(18)2020).
文摘Drought causes water shortage and consequent retardation of plants growth and development.Therefore,improving the drought tolerance of plants is necessary for expanding cultivation and resource promotion.Increasing evidence indicates that phospholipase is involved in the response of plants to drought stress.The objective of this study was to create new drought-tolerant chrysanthemum germplasm,which lays a foundation for the study of the molecular mechanism of phospholipase mediated stress response in chrysanthemum.CmPLDαhas the closest relationship with sunflower HaPLDα,and belongs to the PLDαfamily.CmPLDαover-expressing plants showed a slight shrinking under 20%PEG6000 treatment.The survival rate increased significantly by 1.7−1.8 times that of the wild type.Relative water content(RWC)of CmPLDαover-expressing plants were nearly 10%higher than that of the wild type.Relative electrical conductivity and MDA content were significantly lower than those of the wild type.ABA content of the over-expression lines Z1,Z2 were 1.3 and 1.22 times that of wild type,but ABA content of antisense lines F1,F2 was approximately 0.83 and 0.81 of those of wild type.Most plants of antisense transgenic lines F1,F2 were wrinkled,with a wilting index of 5 and 6,and the survival rate was also lower than that of the wild type after recovery growth.RWC of antisense lines were lower than over-expression lines,relative electrical conductivity and MDA content were significantly higher than those of the wild type.In summary,CmPLDαcould enhance tolerance of chrysanthemum to drought conditions.
