Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,...Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.展开更多
The heterojunctions between metal and polymer have become the effective ways to produce the lighter,safer and more environmental friendly vehicles for the manufacturing fields of automotive and aerospace.The state-of-...The heterojunctions between metal and polymer have become the effective ways to produce the lighter,safer and more environmental friendly vehicles for the manufacturing fields of automotive and aerospace.The state-of-the-art frictionbased welding techniques are characterized by low peak temperature,severe plastic deformation,energy efficiency and nonpollution,which can simultaneously realize the mechanical and chemical bonding,improving mechanical performances.In this review,the current progress about friction-based welding techniques is summarized,containing technical development,welding tool design,microstructural characteristic,process optimization,surface modification and joining mechanism.The conclusions and prospects are presented,which focus on the practical implications for the manufacturing sectors and recommendations for further research and development.The purpose of this review is to elucidate the benefits of friction-based welding techniques so that these methods may be better exploited and industrialized.展开更多
基金supported by the National Key Research and Development Plan(2018YFC2001500)National Natural Science Foundation of China(81972254,82172098).
文摘Skeletal muscle disorders have posed great threats to health.Selective delivery of drugs and oligonucleotides to skeletal muscle is challenging.Aptamers can improve targeting efficacy.In this study,for the first time,the human skeletal muscle-specific ssDNA aptamers(HSM01,etc.)were selected and identified with Systematic Evolution of Ligands by Exponential Enrichment(SELEX).The HSM01 ssDNA aptamer preferentially interacted with human skeletal muscle cells in vitro.The in vivo study using tree shrews showed that the HSM01 ssDNA aptamer specifically targeted human skeletal muscle cells.Furthermore,the ability of HSM01 ssDNA aptamer to target skeletal muscle cells was not affected by the formation of a disulfide bond with nanoliposomes in vitro or in vivo,suggesting a potential new approach for targeted drug delivery to skeletal muscles via liposomes.Therefore,this newly identified ssDNA aptamer and nanoliposome modification could be used for the treatment of human skeletal muscle diseases.
基金supported by the National Natural Science Foundation of China(Nos.52205350 and 52175301)the China Postdoctoral Science Foundation(Nos.2021M690820 and 2021T140151)and the Heilongjiang Postdoctoral Fund(LBH-Z20055).
文摘The heterojunctions between metal and polymer have become the effective ways to produce the lighter,safer and more environmental friendly vehicles for the manufacturing fields of automotive and aerospace.The state-of-the-art frictionbased welding techniques are characterized by low peak temperature,severe plastic deformation,energy efficiency and nonpollution,which can simultaneously realize the mechanical and chemical bonding,improving mechanical performances.In this review,the current progress about friction-based welding techniques is summarized,containing technical development,welding tool design,microstructural characteristic,process optimization,surface modification and joining mechanism.The conclusions and prospects are presented,which focus on the practical implications for the manufacturing sectors and recommendations for further research and development.The purpose of this review is to elucidate the benefits of friction-based welding techniques so that these methods may be better exploited and industrialized.
