Traditional matrix does not allow matrix-assisted laser desorption/ionization mass spectrometry(MALDI MS) to analyze volatile compounds,because volatile analytes may vaporize during the sample preparation process or i...Traditional matrix does not allow matrix-assisted laser desorption/ionization mass spectrometry(MALDI MS) to analyze volatile compounds,because volatile analytes may vaporize during the sample preparation process or in the high vacuum circumstance of ion source.Herein,we reported a Co and N doped porous carbon material(Co-NC) which were synthesized by pyrolysis of a Schiff base coordination compound.Co-NC could simultaneously act as adsorbent of volatile compounds and as matrix of MALDI MS,to provide the capability of MALDI MS to analyze volatile compounds.As adsorbent,Co-NC could stro ngly adsorb and enrich the volatile compounds in perfume and herbs,and hold them even in the high vacuum circumstance.On the other hand,Co-NC could absorb the energy of the laser,and then transfer the energy to the analyte for desorption and ionization of analyte in both negative and positive ionization modes.Additionally,the background interferences were avoided in the low-mass region(<500 Da) when using Co-NC as matrix,overcoming the challenges of MALDI MS analysis of small molecule compounds.In summary,Co-NC as matrix tremendously extended the application of MALDI MS.展开更多
Functional fullerene derivatives exhibit special inhibitory effects on tumor progress and metastasis via diverse tumor microenvironment regulations,while the elusive molecular mechanisms hinder their clinical transfor...Functional fullerene derivatives exhibit special inhibitory effects on tumor progress and metastasis via diverse tumor microenvironment regulations,while the elusive molecular mechanisms hinder their clinical transformation.Herein,it is initially revealed that nanosize aminated fullerene(C_(70)-EDA)can activate autophagic flux,induce G0/G1 cell cycle arrest to abrogate cancer cell proliferation,and significantly inhibit tumor growth in vivo.Mechanismly,C_(70)-EDA promotes the expression of cathepsin D involved in autophagic activation via post-transcriptional regulation,attributing to the interaction with a panel of RNA binding proteins.The accumulation of cathepsin D induces the autophagic degradation of cyclin D1,which arouses G0/G1 phase arrest.This work unveils the fantastic anti-tumor activity of aminated fullerene,elucidates the molecular mechanism,and provides a new strategy for the antineoplastic drug development on functional fullerenes.展开更多
基金supported by the National Key R&D Program of China(No.2018YFA0506900)National Natural Science Foundation of China(Nos.21635008 and 21621062)the Military Medicine and Healthy Major Project,China(No.AWS16J016)。
文摘Traditional matrix does not allow matrix-assisted laser desorption/ionization mass spectrometry(MALDI MS) to analyze volatile compounds,because volatile analytes may vaporize during the sample preparation process or in the high vacuum circumstance of ion source.Herein,we reported a Co and N doped porous carbon material(Co-NC) which were synthesized by pyrolysis of a Schiff base coordination compound.Co-NC could simultaneously act as adsorbent of volatile compounds and as matrix of MALDI MS,to provide the capability of MALDI MS to analyze volatile compounds.As adsorbent,Co-NC could stro ngly adsorb and enrich the volatile compounds in perfume and herbs,and hold them even in the high vacuum circumstance.On the other hand,Co-NC could absorb the energy of the laser,and then transfer the energy to the analyte for desorption and ionization of analyte in both negative and positive ionization modes.Additionally,the background interferences were avoided in the low-mass region(<500 Da) when using Co-NC as matrix,overcoming the challenges of MALDI MS analysis of small molecule compounds.In summary,Co-NC as matrix tremendously extended the application of MALDI MS.
基金This work was supported by the National Natural Science Foundation of China(No.51802310)All animal experiments were conducted according to protocols approved by the Institutional Animal Care and Use Committee in the Institute of Chemistry,Chinese Academy of Sciences.
文摘Functional fullerene derivatives exhibit special inhibitory effects on tumor progress and metastasis via diverse tumor microenvironment regulations,while the elusive molecular mechanisms hinder their clinical transformation.Herein,it is initially revealed that nanosize aminated fullerene(C_(70)-EDA)can activate autophagic flux,induce G0/G1 cell cycle arrest to abrogate cancer cell proliferation,and significantly inhibit tumor growth in vivo.Mechanismly,C_(70)-EDA promotes the expression of cathepsin D involved in autophagic activation via post-transcriptional regulation,attributing to the interaction with a panel of RNA binding proteins.The accumulation of cathepsin D induces the autophagic degradation of cyclin D1,which arouses G0/G1 phase arrest.This work unveils the fantastic anti-tumor activity of aminated fullerene,elucidates the molecular mechanism,and provides a new strategy for the antineoplastic drug development on functional fullerenes.