Protein corona mediated liposomal drug delivery for bacterial infection management

Liposomes have been widely investigated as a class of promising antibiotic delivery systems for the treatment of life-threatening bacterial infections.However,the inevitable formation of protein corona on the liposomal surface can heavily impact in vivo performance.A better understanding of the effects of protein corona on liposomal behavior can significantly improve antibacterial liposomal drug development.Here,the critical role of protein corona in mediating liposome-bacteria interactions was elucidated.Adsorption of negatively charged protein on cationic liposome weakened electrostatic attractionenhanced liposomal binding to the bacteria.Cumulative complement deposition on anionic liposome composed of phosphatidylglycerol(DSPG s Lip)contributed to a superior binding affinity of DSPG s Lip to planktonic bacteria and biofilms,which was exploited to enhance bacteria-targeted drug delivery.In both S.aureus-related osteomyelitis and pneumonia mice models,DSPG s Lip was demonstrated as a promising antibiotic nanocarrier for managing MRSA infection,indicating the benefits of lipid composition-based protein corona modulation in liposomal antibiotic delivery for bacterial infection treatment.

Hypoxia-degradable and long-circulating zwitterionic phosphorylcholine-based nanogel for enhanced tumor drug delivery

Tumor microenvironment has been widely utilized for advanced drug delivery in recent years,among which hypoxia-responsive drug delivery systems have become the research hotspot.Although hypoxia-responsive micelles or polymersomes have been successfully developed,a type of hypoxia-degradable nanogel has rarely been reported and the advantages of hypoxia-degradable nanogel over other kinds of degradable nanogels in tumor drug delivery remain unclear.Herein,we reported the synthesis of a novel hypoxia-responsive crosslinker and the fabrication of a hypoxia-degradable zwitterionic poly(phosphorylcholine)-based(HPMPC)nanogel for tumor drug delivery.The obtained HPMPC nanogel showed ultra-long blood circulation and desirable immune compatibility,which leads to high and long-lasting accumulation in tumor tissue.Furthermore,HPMPC nanogel could rapidly degrade into oligomers of low molecule weight owing to the degradation of azo bond in hypoxic environment,which leads to the effective release of the loaded drug.Impressively,HPMPC nanogel showed superior tumor inhibition effect both in vitro and in vivo compared to the reduction-responsive phosphorylcholine-based nanogel,owing to the more complete drug release.Overall,the drug-loaded HPMPC nanogel exhibits a pronounced tumor inhibition effect in a humanized subcutaneous liver cancer model with negligible side effects,which showed great potential as nanocarrier for advanced tumor drug delivery.