AIM：To explore the effect of miR-22 on viability,migration,invasion and apoptosis in retinoblastoma（RB）Y79 cells and to further detect the potential mechanism.METHODS：Plasmids were constructed to change the expres...AIM：To explore the effect of miR-22 on viability,migration,invasion and apoptosis in retinoblastoma（RB）Y79 cells and to further detect the potential mechanism.METHODS：Plasmids were constructed to change the expression level of miR-22 in Y79 cells. Real-time reverse transcription polymerase chain reaction（RT-PCR）was conducted to test the expression level of miR-22. After changing the expression of miR-22,the mRNA and protein levels of high-mobility group box 1（HMGB1） were investigated using RT-PCR and Western blotting.The effect of miR-22 on viability was analyzed by using cell counting kit-8（CCK-8） assay and the effect on apoptosis was detected by the flow cytometry. Wound healing migration assay and Transwell invasion assay were used to detect the effects of miR-22 on cell motility.RESULTS：miR-22 inhibited viability,migration and invasion,while promoting apoptosis,in RB Y79 cells.The inhibition rate of miR-22 overexpression group at 12,24,48h was 11.71%±2.54%,21.36%±1.39% and 29.44%±1.15%,respectively.Cellular apoptosis was higher in miR-22 overexpression group（17.00%±0.39%） compared with negative control（4.38%±0.38%）.miR-22 negatively mediated the expression of HMGB1.Furthermore,decreased展开更多
基金Supported by the Key Project of Anhui Provincial Excellent Young Talent Support Program(No.gxyqZD2017033)the Grant of Second Affiliated Hospital of Anhui Medical University(No.2014BKJ047)the Project of Anhui Provincial Young Wanjiang Scholars Support Program(No.9101041203)
文摘AIM：To explore the effect of miR-22 on viability,migration,invasion and apoptosis in retinoblastoma（RB）Y79 cells and to further detect the potential mechanism.METHODS：Plasmids were constructed to change the expression level of miR-22 in Y79 cells. Real-time reverse transcription polymerase chain reaction（RT-PCR）was conducted to test the expression level of miR-22. After changing the expression of miR-22,the mRNA and protein levels of high-mobility group box 1（HMGB1） were investigated using RT-PCR and Western blotting.The effect of miR-22 on viability was analyzed by using cell counting kit-8（CCK-8） assay and the effect on apoptosis was detected by the flow cytometry. Wound healing migration assay and Transwell invasion assay were used to detect the effects of miR-22 on cell motility.RESULTS：miR-22 inhibited viability,migration and invasion,while promoting apoptosis,in RB Y79 cells.The inhibition rate of miR-22 overexpression group at 12,24,48h was 11.71%±2.54%,21.36%±1.39% and 29.44%±1.15%,respectively.Cellular apoptosis was higher in miR-22 overexpression group（17.00%±0.39%） compared with negative control（4.38%±0.38%）.miR-22 negatively mediated the expression of HMGB1.Furthermore,decreased
