Micro RNA-26a(miR-26a)has been verified to promote osteogenic differentiation of mesenchymal stem cells in recent years.The main obstacles to its application in bone regeneration are instability in the physiological e...Micro RNA-26a(miR-26a)has been verified to promote osteogenic differentiation of mesenchymal stem cells in recent years.The main obstacles to its application in bone regeneration are instability in the physiological environment and low efficiency of cellular membrane penetration.To overcome these problems,we constructed a novel plant virus gene delivery system based on Cowpea chlorotic mottle virus(CCMV).By encapsulating miR-26a with purified capsid protein(CP)dimers derived from CCMV,CPmiR-26a(CP26a)virus-like particles(VLPs)were obtained.CP26a retained a structure similar to the native CCMV and protected miR-26a from digestion with its exterior CP.Moreover,CP26a featured similar cellular uptake efficiency,osteogenesis promotion ability,and better biocompatibility compared with Lipofectamine2000-miR-26a(lipo26a),which indicated a promising prospect for CCMV as a novel gene delivery system.展开更多
Stem cells have been one of the ideal sources for tissue regeneration owing to their capability of self-renewal and differentiation.In vivo,the extracellular microenvironment plays a vital role in modulating stem cell...Stem cells have been one of the ideal sources for tissue regeneration owing to their capability of self-renewal and differentiation.In vivo,the extracellular microenvironment plays a vital role in modulating stem cell fate.When developing biomaterials for regenerative medicine,incorporating biochemical and biophysical cues to mimic extracellular matrix can enhance stem cell lineage differentiation.More specifically,modulating the stem cell fate can be achieved by controlling the nanotopographic features on synthetic surfaces.Optimization of nanotopographical features leads to desirable stem cell functions,which can maximize the effectiveness of regenerative treatment.In this review,nanotopographical surfaces,including static patterned surface,dynamic patterned surface,and roughness are summarized,and their fabrication,as well as the impact on stem cell behaviour,are discussed.Later,the recent progress of applying nanotopographical featured biomaterials for altering different types of stem cells is presented,which directs the design and fabrication of functional biomaterial.Last,the perspective in fundamental research and for clinical application in this field is discussed.展开更多
基金supported by the research funding from West China School/Hospital of Stomatology,Sichuan University(Nos.RCDWJS2021-15,RD-03-202010,RD-02-202004)the research funding from the State Key Laboratory of Oral Diseases(No.SKLOD202111)the fellowship of China Postdoctoral Science Foundation(No.2020TQ0211)。
文摘Micro RNA-26a(miR-26a)has been verified to promote osteogenic differentiation of mesenchymal stem cells in recent years.The main obstacles to its application in bone regeneration are instability in the physiological environment and low efficiency of cellular membrane penetration.To overcome these problems,we constructed a novel plant virus gene delivery system based on Cowpea chlorotic mottle virus(CCMV).By encapsulating miR-26a with purified capsid protein(CP)dimers derived from CCMV,CPmiR-26a(CP26a)virus-like particles(VLPs)were obtained.CP26a retained a structure similar to the native CCMV and protected miR-26a from digestion with its exterior CP.Moreover,CP26a featured similar cellular uptake efficiency,osteogenesis promotion ability,and better biocompatibility compared with Lipofectamine2000-miR-26a(lipo26a),which indicated a promising prospect for CCMV as a novel gene delivery system.
基金This work was supported by Research Funding from West China School/Hospital of Stomatology,Sichuan University(Nos.RD-03-202010,RCDWJS2021-15,RD-02-202004)the China Postdoctoral Science Foundation(No.2020TQ0211).
文摘Stem cells have been one of the ideal sources for tissue regeneration owing to their capability of self-renewal and differentiation.In vivo,the extracellular microenvironment plays a vital role in modulating stem cell fate.When developing biomaterials for regenerative medicine,incorporating biochemical and biophysical cues to mimic extracellular matrix can enhance stem cell lineage differentiation.More specifically,modulating the stem cell fate can be achieved by controlling the nanotopographic features on synthetic surfaces.Optimization of nanotopographical features leads to desirable stem cell functions,which can maximize the effectiveness of regenerative treatment.In this review,nanotopographical surfaces,including static patterned surface,dynamic patterned surface,and roughness are summarized,and their fabrication,as well as the impact on stem cell behaviour,are discussed.Later,the recent progress of applying nanotopographical featured biomaterials for altering different types of stem cells is presented,which directs the design and fabrication of functional biomaterial.Last,the perspective in fundamental research and for clinical application in this field is discussed.
