Insufficient sleep can produce a multitude of deleterious repercussions on various domains of human well-being.Concomitantly,the walnut(Juglans mandshurica)confers numerous salutary biological activities pertaining to...Insufficient sleep can produce a multitude of deleterious repercussions on various domains of human well-being.Concomitantly,the walnut(Juglans mandshurica)confers numerous salutary biological activities pertaining to sleep.Nevertheless,the sedative and hypnotic capacities of walnut's functional constituents remain obscure.In this investigation,we analyzed the sedative and hypnotic components of the walnut Diaphragma juglandis fructus and innovatively discovered a compound,defined as 3-hydroxy-4-iminobutyric acid(HIBA),which disrupts motor activity and enhances sleep duration by regulating the neurotransmitters(GABA,DA,etc.)within the brain and serum of mice.Subsequently,a metabolomics approach of the serum,basal ganglia,hypothalamus,and hippocampus as well as the gut microbiota was undertaken to unravel the underlying molecular mechanisms of sleep promotion.Our data reveal that HIBA can regulate the metabolism of basal ganglia(sphingolipids,acylcarnitines,etc.),possibly in relation to HIBA's influence on the gut microbiome(Muribaculum,Bacteroides,Lactobacillus,etc.).Therefore,we introduce a novel natural product,HIBA,and explicate the modulation of sleep promotion in mice based on the microbiota-gut-brain axis.This study contributes fresh insights toward natural product-based sleep research.展开更多
基金supported by the Key R&D program project of Yunnan Province(202202AE090007)the Science and Technology Project of Yunnan Province(202305AF150025)+2 种基金the Science and Technology Project of Chongzuo City(20210710)the Fundamental Research Funds for the Central Universities(JUSRP222001)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Jiangnan University.
文摘Insufficient sleep can produce a multitude of deleterious repercussions on various domains of human well-being.Concomitantly,the walnut(Juglans mandshurica)confers numerous salutary biological activities pertaining to sleep.Nevertheless,the sedative and hypnotic capacities of walnut's functional constituents remain obscure.In this investigation,we analyzed the sedative and hypnotic components of the walnut Diaphragma juglandis fructus and innovatively discovered a compound,defined as 3-hydroxy-4-iminobutyric acid(HIBA),which disrupts motor activity and enhances sleep duration by regulating the neurotransmitters(GABA,DA,etc.)within the brain and serum of mice.Subsequently,a metabolomics approach of the serum,basal ganglia,hypothalamus,and hippocampus as well as the gut microbiota was undertaken to unravel the underlying molecular mechanisms of sleep promotion.Our data reveal that HIBA can regulate the metabolism of basal ganglia(sphingolipids,acylcarnitines,etc.),possibly in relation to HIBA's influence on the gut microbiome(Muribaculum,Bacteroides,Lactobacillus,etc.).Therefore,we introduce a novel natural product,HIBA,and explicate the modulation of sleep promotion in mice based on the microbiota-gut-brain axis.This study contributes fresh insights toward natural product-based sleep research.
