The fruit peel color is a crucial trait of cucumber.To better understand the molecular mechanisms underlying cucumber peel coloration,we compared the UPLC-ESI-MS/MS-based flavonoid metabolomic and RNA sequencing-based...The fruit peel color is a crucial trait of cucumber.To better understand the molecular mechanisms underlying cucumber peel coloration,we compared the UPLC-ESI-MS/MS-based flavonoid metabolomic and RNA sequencing-based transcriptomic profiling of the brown peeled cucumber line‘PW’at six developmental stages.A total of 210 flavonoid metabolites were identified.Of which,117 flavonoid metabolites were differentially accumulated.In this study,weighted gene co-expression network analysis combined with Kyoto Encyclopedia of Gene and Genomes enrichment analysis revealed key genes coding for seven enzymes and eight transcription factors(TFs)associated with flavonoid biosynthesis.Among them,the R2R3MYB CsaV3_4G001130 is the best candidate gene that is responsible for controlling mature fruit colors in cucumber.Sanger sequencing revealed one nonsynonymous SNP in the exon of CsaV3_4G001130 among the selected 11 cucumber lines,which introduced a premature stop codon,generating a truncated protein in pale yellow or creamy peeled fruits.Yeast two-hybrid assays showed a direct interaction of CsaV3_4G001130 with the bHLH TF CsaV3_1G002260 and the WD40 protein CsaV3_5G001800.However,the interactions were influenced by the nonsynonymous SNP we identified.Our finding revealed that the integrated transcriptome and metabolome analysis further demonstrated that the abundance of some pigmented flavonoids(especially anthocyanins and chalcones)contributed to the coloration of‘PW’fruits.These findings pave the way for elucidation of flavonoid biosynthesis and improvement of cucumber peel color in the future.展开更多
Purpose:To investigate the pathological characteristics of congenital macular retinoschisis by optic coherence tomography (OCT).Methods:The data of 7 cases(14 eyes) with congenital macular retinoschisis were collected...Purpose:To investigate the pathological characteristics of congenital macular retinoschisis by optic coherence tomography (OCT).Methods:The data of 7 cases(14 eyes) with congenital macular retinoschisis were collected.Electroretinogram (ERG),fundus fluorecein angiography (FFA) and OCT examination were performed,respectively.Results:The OCT images showed schisis cavity in all eyes.Schisis was confined to the fovea and parafovea in 2 eyes (1 patient).Schisis was involved in entire macular area in 12 eyes (6 patients).Inner nuclear layer (INL) schisis was seen in all eyes.Schisis was located at both INL and outer nuclear layer.(ONL)/outer plexiform layer (OPL) in 2 of the 14 eyes.Besides the schisis cavity,small cysts within ganglion cell layer were found in 3 eyes.The small cysts were confined to parafoveal area.The OCT images of both eyes in one patient were similar but not exactly the same or symmetrical.Conclusion:Morphology,extension and schisis location in congenital macular retinoschisis have respective diversities.展开更多
基金The‘JBGS’Project of Seed Industry Revitalization in Jiangsu Province[Grant No.JBGS(2021)018]the Jiangsu Agricultural Innovation of New Cultivars(Grant No.PZCZ201720)+2 种基金the Jiangsu Agriculture Science and Technology Innovation Fund[Grant No.CX(20)3103]the Open Project Program of Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement(Grant No.K2020030)the Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety,the Ministry of Education of China(Grant No.JILAR-KF202001).
文摘The fruit peel color is a crucial trait of cucumber.To better understand the molecular mechanisms underlying cucumber peel coloration,we compared the UPLC-ESI-MS/MS-based flavonoid metabolomic and RNA sequencing-based transcriptomic profiling of the brown peeled cucumber line‘PW’at six developmental stages.A total of 210 flavonoid metabolites were identified.Of which,117 flavonoid metabolites were differentially accumulated.In this study,weighted gene co-expression network analysis combined with Kyoto Encyclopedia of Gene and Genomes enrichment analysis revealed key genes coding for seven enzymes and eight transcription factors(TFs)associated with flavonoid biosynthesis.Among them,the R2R3MYB CsaV3_4G001130 is the best candidate gene that is responsible for controlling mature fruit colors in cucumber.Sanger sequencing revealed one nonsynonymous SNP in the exon of CsaV3_4G001130 among the selected 11 cucumber lines,which introduced a premature stop codon,generating a truncated protein in pale yellow or creamy peeled fruits.Yeast two-hybrid assays showed a direct interaction of CsaV3_4G001130 with the bHLH TF CsaV3_1G002260 and the WD40 protein CsaV3_5G001800.However,the interactions were influenced by the nonsynonymous SNP we identified.Our finding revealed that the integrated transcriptome and metabolome analysis further demonstrated that the abundance of some pigmented flavonoids(especially anthocyanins and chalcones)contributed to the coloration of‘PW’fruits.These findings pave the way for elucidation of flavonoid biosynthesis and improvement of cucumber peel color in the future.
文摘Purpose:To investigate the pathological characteristics of congenital macular retinoschisis by optic coherence tomography (OCT).Methods:The data of 7 cases(14 eyes) with congenital macular retinoschisis were collected.Electroretinogram (ERG),fundus fluorecein angiography (FFA) and OCT examination were performed,respectively.Results:The OCT images showed schisis cavity in all eyes.Schisis was confined to the fovea and parafovea in 2 eyes (1 patient).Schisis was involved in entire macular area in 12 eyes (6 patients).Inner nuclear layer (INL) schisis was seen in all eyes.Schisis was located at both INL and outer nuclear layer.(ONL)/outer plexiform layer (OPL) in 2 of the 14 eyes.Besides the schisis cavity,small cysts within ganglion cell layer were found in 3 eyes.The small cysts were confined to parafoveal area.The OCT images of both eyes in one patient were similar but not exactly the same or symmetrical.Conclusion:Morphology,extension and schisis location in congenital macular retinoschisis have respective diversities.