Hepatitis B virus(HBV),an enveloped DNA virus with a partially double-stranded DNA genome,causes transient and chronic hepatitis B in humans.A certain amount of chronic HBV infections eventually leads to liver cirrhos...Hepatitis B virus(HBV),an enveloped DNA virus with a partially double-stranded DNA genome,causes transient and chronic hepatitis B in humans.A certain amount of chronic HBV infections eventually leads to liver cirrhosis or hepatocellular carcinoma which is a major health problem worldwide[1,2].With tissue tropism and species-specific infection,the establishment of in vitro models permissive to HBV is critical in understanding the viral life cycle and designing curative therapies.The ideal cell model for in vitro infection studies is primary human hepatocytes(PHHs)[3].However,PHHis difficult to obtain andwith high cost.The donor diversity and high batch-to-batch variability of infection efficiency also limit its applicability.In 2012,sodium taurocholate co-transporting polypeptide(NTCP)was identified as a functional receptor for HBV entry[4].Thus,a new infection model based on NTCP-reconstituted HepG2 hepatocytes(HepG2-NTCP)was successfully established,which greatly promoted the understanding of HBV infection and replication[4].However,in this infection system,successful HBV infection stringently requires a high concentration of HBV viral particles,but still in most cases supports a low level of HBV replication[5].Thus,further optimization of infection cell models is an important demand in HBV research.展开更多
基金supported by the National Natural Science Foundation of China(32222022,92354301,92054104)Key Research and Development Program,Ministry of Science and Technology of China(2022YFC2303502,2021YFC2300204)Natural Science Foundation of Shanghai(23ZR1470900).
文摘Hepatitis B virus(HBV),an enveloped DNA virus with a partially double-stranded DNA genome,causes transient and chronic hepatitis B in humans.A certain amount of chronic HBV infections eventually leads to liver cirrhosis or hepatocellular carcinoma which is a major health problem worldwide[1,2].With tissue tropism and species-specific infection,the establishment of in vitro models permissive to HBV is critical in understanding the viral life cycle and designing curative therapies.The ideal cell model for in vitro infection studies is primary human hepatocytes(PHHs)[3].However,PHHis difficult to obtain andwith high cost.The donor diversity and high batch-to-batch variability of infection efficiency also limit its applicability.In 2012,sodium taurocholate co-transporting polypeptide(NTCP)was identified as a functional receptor for HBV entry[4].Thus,a new infection model based on NTCP-reconstituted HepG2 hepatocytes(HepG2-NTCP)was successfully established,which greatly promoted the understanding of HBV infection and replication[4].However,in this infection system,successful HBV infection stringently requires a high concentration of HBV viral particles,but still in most cases supports a low level of HBV replication[5].Thus,further optimization of infection cell models is an important demand in HBV research.
