MicroRNA-29a attenuates angiotensin-Ⅱ induced-left ventricular remodeling by inhibiting collagen,TGF-β and SMAD2/3 expression

Background Left ventricular(LV)remodeling is the most common target organ damage in hypertension.Previously,our study found that plasma microRNA-29a(miR-29a)level was associated with the LV remodeling in hypertensive patients.However,the causal relationship between miR-29a and LV remodeling remains unknown.Thus,the aim of this study was to investigate the regulation mechanism of miR-29a in LV remodeling.Methods&Results Overexpression and knockdown miR-29a mice were generated by tail-intravenous injection of miR-29a-mimic and inhibitor lentivirus for one week respectively.Then the mice were subjected to angiotensin-II(AngII)induced LV remodeling by subcutaneous AngII capsule osmotic pumping into AngII for four weeks.AngII-induced LV remodeling mice as the model group(n=9).Age-matched male SPF C57/BL6J mice(6–8 weeks old)were treated with the pumping of saline as a vehicle(n=6).In vivo,overexpression miR-29a ameliorated AngII-induced LV remodeling,while knockdown miR-29a deteriorated LV remodeling.Simultaneously,we observed that overexpression miR-29a mice inhibited but knockdown miR-29a mice increased cardiac cross-sectional area,indicating that miR-29a has an antagonistic effect on cardiac hypertrophy.Further studies found that overexpression miR-29a inhibited the content of the LV collagen including collagen I and III.Moreover,the expression of transforming growth factor-β(TGF-β)and phosphorylated SMAD2/3 decreased with the down-regulation of collagen I and III in overexpression miR-29a mice.Conclusions Our finding indicates that overexpression miR-29a attenuates LV remodeling by inhibiting collagen deposition,TGF-β,and phosphorylated SMAD2/3 expression.Thus,intervention miR-29a may be a therapeutic target for attenuating LV remodeling.

A world's first attempt of mixed-reality system guided inferior vena cava filter implantation under remote guidance of 5G communication

Deep vein thrombosis(DVT)is a manifestation of venous thromboembolism,which can result from long-term bed and lead to pulmonary embolism(PE),stroke,mesenteric artery embolis,etc.Patients after surgery without bed-off activity may prone to thrombosis,but traditional anticoagulant therapy is a contraindication for them because of high risk of postoperative hemorrhage.Inferior vena cava filter(IVCF)implantation is the most effective method to intercept thrombosis from lower limb and to prevent PE.However,radiation and contrast agent limit its application.Our team have successfully performed an IVCF implantation guided by a mixed-reality(MR)system,[1]which is a chance for critically ill patients to allow the IVCF implantation exposed under no Xray and require no contrast agent.5G can help remote holographic projection images between distant range,meeting the needs of experts to guide the operation of critically ill patients.We describe a case of weak woman after neurosurgery who can not tolerate traditional interventional surgery to prevent DVT from falling off.We performed a mixed-reality system guided IVCF implantation under remote guidance of 5G communication.

Association between dietary sodium intake and blood pressure variability in Chinese patients with hypertension

Background:The association between dietary sodium intake and blood pressure variability(BPV)in hypertensive patients remains unclear.The objective of this study was to demonstrate whether dietary sodium intake is a predictor of elevated BPV in Chinese patients with hypertension.Methods:A total of 235 patients with essential hypertension were enrolled in the Department of Cardiology,Chinese People’s Liberation Army(PLA)General Hospital in 2018 to 2019,all of whom underwent 24-h ambulatory blood pressure monitoring.BPV was calculated as the standard deviation(SD),coefficient of variation(CV),variation independent of mean(VIM)of blood pressure measurements,respectively,and divided into diurnal systolic BPV(SBPV),diurnal diastolic BPV(DBPV),nocturnal SBPV,and nocturnal DBPV.24-h urine samples were collected to measure 24-h urine sodium excretion,which represents dietary sodium intake.The relationship between dietary sodium intake and BPV was analyzed by using Spearman correlations and multiple linear regression analysis.Results:Nocturnal SBPV-SD,CV,VIM,and nocturnal DBPV-SD in the high urine sodium excretion group were significantly higher than those in the medium and low urine sodium excretion groups,whereas diurnal SBPV-SD,CV,VIM,diurnal DBPV-SD,CV,VIM,and nocturnal DBPV-CV,VIM were not.Using the Spearman correlation analysis,we found a linear correlation between 24-h urine sodium excretion and nocturnal SBPV-SD,CV,VIM(SD,r=0.22,P=0.001;CV,r=0.17,P=0.009;VIM,r=0.16,P=0.020),nocturnal DBPV-SD(r=0.21,P=0.001),respectively.After further adjusting for confounding factors by multiple linear regression,the positive correlations remained between 24-h urine sodium excretion and nocturnal SBPV-SD,CV,VIM(SD,β=0.224,P<0.001;CV,β=0.211,P=0.001;VIM,β=0.213,P=0.001),nocturnal DBPV(SD,β=0.215,P=0.001),respectively.Conclusions:Dietary sodium intake is associated with nocturnal SBPV in Chinese patients with hypertension.