Lycium barbarum polysaccharides promotes in vivo proliferation of adult rat retinal progenitor cells

Lycium barbarum is a widely used Chinese herbal medicine prescription for protection of optic nerve.However,it remains unclear regarding the effects of Lycium barbarum polysaccharides,the main component of Lycium barbarum,on in vivo proliferation of adult ciliary body cells.In this study,adult rats were intragastrically administered low-and high-dose Lycium barbarum polysaccharides（1 and 10 mg/kg）for 35 days and those intragastrically administered phosphate buffered saline served as controls.The number of Ki-67-positive cells in rat ciliary body in the Lycium barbarum polysaccharides groups,in particular low-dose Lycium barbarum polysaccharides group,was significantly greater than that in the phosphate buffered saline group.Ki-67-positive rat ciliary body cells expressed nestin but they did not express glial fibrillary acidic protein.These findings suggest that Lycium barbarum polysaccharides can promote the proliferation of adult rat retinal progenitor cells and the proliferated cells present with neuronal phenotype.

Life Cycle Assessment-based method of Excessive Commodity Packaging on Energy Conservation and Emissions Reduction

Based on a Life Cycle Assessment model, the effect of the whole life cycle of excessive commodity packaging on the environment was analyzed by eBalance Evaluation LCA software from production through circulation. The cost evaluation system and environment impact model of its three main processes, that is, material production, product machining and waste manufacturing, were established to identify the main environmental impact corresponding indicators and the influence on energy conservation and emissions reduction of excessive packaging life cycle. And packaging of moon-cakes was taken as an example to analyze the difference between the ordinary packaging and excessive packaging of moon-cakes in terms of life cycle assessment and costs analysis. Meanwhile some measures are put forward to conserve energy and reduce emissions.

Engineering a cationic supramolecular charge switch for facile amino acids enantiodiscrimination based on extended-gate field effect transistors

Chiral recognition of essential amino acids(EAAs)is a huge challenge that keeps plaguing analytical scientists due to their cryptochirality and limited steric interaction sites.Inspired by the superior enantioselectivity of functional supramolecular cyclodextrins(CDs)and strong signal amplification ability of field effect transistors(FETs),this work firstly reports a cationic supramolecular charge switch for facile enantiodiscrimination of EAAs based on extended-gate organic FET(EG-OFET).The cationic phenylcarbamoylated-CD single isomer acts as a charge switch via interacting with different enantiomers and the weak stereo-differentiation intermolecular interaction signals between the cationic perphenylcarbamoylated CDs and EAAs on the EG can be strongly and rapidly amplified through an OFET.Efficient chiral differentiation of six EAAs,including phenylalanine,tryptophan,leucine,isoleucine,lysine and valine,are successfully achieved without any derivation process and the detection limit for D-phenylalanine is down to 10^(-13)mol/L.We believe that this study provides a new and facile sensing perspective for natural amino acids and may afford deeper understanding of molecular chirality.