Recovery of functional beta cell mass offers a biological cure for type 1 diabetes. However, beta cell mass is difficult to regain once lost since the proliferation rate of beta cells after youth is very low. Angiopoi...Recovery of functional beta cell mass offers a biological cure for type 1 diabetes. However, beta cell mass is difficult to regain once lost since the proliferation rate of beta cells after youth is very low. Angiopoietin like-protein 8 (ANGPTL8), a peptide that has a role in the regulation of lipoprotein lipase activity, was reported to increase beta cell proliferation in mice in 2013. Subsequent studies of human ANGPTL8 for short term (3 to 8 days) in non-diabetic mice showed little or no increase in beta cell proliferation. Here, we examined the effect of ANGPTL8 on glucose homeostasis in models that have not been examined previously. We expressed mouse ANGPTL8 using adenovirus in 2 mouse models of diabetes (streptozotocin and Non-Obese Diabetic (NOD) mice) over 2 weeks. Also, we tested ANGPTL8 in NOD mice deficient in leukocyte 12-lipoxygenase (12LO), an enzyme that contributes to insulitis and loss of beta cell function in NOD, in an effort to determine whether 12LO deficiency alters the response to ANGPTL8. Adenovirus-mediated expression of ANGPTL8 lowered blood glucose levels in streptozotocin treated mice without an increase in beta cell proliferation or serum insulin concentration. While ANGPTL8 did not reverse hyperglycemia in overtly hyperglycemic NOD mice or alter glucose homeostasis of non-diabetic NOD mice, ANGPTL8 reduced blood glucose levels in 12LOKO NOD mice. However, the lower glucose levels in 12LOKO NOD were not associated with higher serum insulin levels or beta cell proliferation. In summary, while mouse ANGPTL8 does not increase beta cell proliferation in NOD mice or streptozotocin treated mice in agreement with studies in non-diabetic mice, it lowers blood glucose levels in multiple low-dose streptozotocin induced diabetes and 12LO deficiency indicating that host factors influence the impact of ANGPTL8 on glucose homeostasis.展开更多
This study was conducted to establish a method for rapid determination of crude starch content in complete quinoa( Chenopodium quinoa Willd) seeds.The near infrared spectra of 100 quinoa samples were collected,and a m...This study was conducted to establish a method for rapid determination of crude starch content in complete quinoa( Chenopodium quinoa Willd) seeds.The near infrared spectra of 100 quinoa samples were collected,and a mathematic model was built using the near infrared spectra within the wavelength range of 1 0 000-4 000 cm^(-1) by first derivative + vector normalization spectral pre-processing. The results showed that the quantification model of starch content had better calibration and prediction effects,and showed a determination coefficient of cross validation( r_(cv)~2) of 0. 914 7 and a determination coefficient of validation( r _(val)~2) of 0. 903 1. The determination of starch content in complete quinoa seeds by near infrared spectroscopy is totally feasible.展开更多
This study was conducted to find a method for rapid determination of fat content in complete quinoa( Chenopodium quinoa Willd) seeds. The near infrared spectra of 100 quinoa samples were collected,and a mathematic mod...This study was conducted to find a method for rapid determination of fat content in complete quinoa( Chenopodium quinoa Willd) seeds. The near infrared spectra of 100 quinoa samples were collected,and a mathematic model was built using the near infrared spectra,so as to perform prediction. The results showed that within the wavelength range of 1 0 000-4 000 cm^(-1),the quantification model of fat content built by first derivative + vector normalization spectral pre-processing had better calibration and prediction effects,and showed a determination coefficient of cross validation( r_(cv)~2 ) of 0. 939 3 and a determination coefficient of validation( r_(val)~2 ) of 0. 923 5. The near infrared spectral model of fat could be used for rapid detection of fat contents in quinoa.展开更多
文摘Recovery of functional beta cell mass offers a biological cure for type 1 diabetes. However, beta cell mass is difficult to regain once lost since the proliferation rate of beta cells after youth is very low. Angiopoietin like-protein 8 (ANGPTL8), a peptide that has a role in the regulation of lipoprotein lipase activity, was reported to increase beta cell proliferation in mice in 2013. Subsequent studies of human ANGPTL8 for short term (3 to 8 days) in non-diabetic mice showed little or no increase in beta cell proliferation. Here, we examined the effect of ANGPTL8 on glucose homeostasis in models that have not been examined previously. We expressed mouse ANGPTL8 using adenovirus in 2 mouse models of diabetes (streptozotocin and Non-Obese Diabetic (NOD) mice) over 2 weeks. Also, we tested ANGPTL8 in NOD mice deficient in leukocyte 12-lipoxygenase (12LO), an enzyme that contributes to insulitis and loss of beta cell function in NOD, in an effort to determine whether 12LO deficiency alters the response to ANGPTL8. Adenovirus-mediated expression of ANGPTL8 lowered blood glucose levels in streptozotocin treated mice without an increase in beta cell proliferation or serum insulin concentration. While ANGPTL8 did not reverse hyperglycemia in overtly hyperglycemic NOD mice or alter glucose homeostasis of non-diabetic NOD mice, ANGPTL8 reduced blood glucose levels in 12LOKO NOD mice. However, the lower glucose levels in 12LOKO NOD were not associated with higher serum insulin levels or beta cell proliferation. In summary, while mouse ANGPTL8 does not increase beta cell proliferation in NOD mice or streptozotocin treated mice in agreement with studies in non-diabetic mice, it lowers blood glucose levels in multiple low-dose streptozotocin induced diabetes and 12LO deficiency indicating that host factors influence the impact of ANGPTL8 on glucose homeostasis.
基金Supported by Special Fund for the Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture(2017NWB036-20)Key Project of Shanxi Academy of Agricultural Sciences(YGG17064)Key Research Plan Project of Shanxi Province(201603D21102)
文摘This study was conducted to establish a method for rapid determination of crude starch content in complete quinoa( Chenopodium quinoa Willd) seeds.The near infrared spectra of 100 quinoa samples were collected,and a mathematic model was built using the near infrared spectra within the wavelength range of 1 0 000-4 000 cm^(-1) by first derivative + vector normalization spectral pre-processing. The results showed that the quantification model of starch content had better calibration and prediction effects,and showed a determination coefficient of cross validation( r_(cv)~2) of 0. 914 7 and a determination coefficient of validation( r _(val)~2) of 0. 903 1. The determination of starch content in complete quinoa seeds by near infrared spectroscopy is totally feasible.
基金Supported by Special Fund for the Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture(2017NWB036-20)Key Project of Shanxi Academy of Agricultural Sciences(YGG17064)Key Research Plan Project of Shanxi Province(201603D21102)
文摘This study was conducted to find a method for rapid determination of fat content in complete quinoa( Chenopodium quinoa Willd) seeds. The near infrared spectra of 100 quinoa samples were collected,and a mathematic model was built using the near infrared spectra,so as to perform prediction. The results showed that within the wavelength range of 1 0 000-4 000 cm^(-1),the quantification model of fat content built by first derivative + vector normalization spectral pre-processing had better calibration and prediction effects,and showed a determination coefficient of cross validation( r_(cv)~2 ) of 0. 939 3 and a determination coefficient of validation( r_(val)~2 ) of 0. 923 5. The near infrared spectral model of fat could be used for rapid detection of fat contents in quinoa.
