Emulation platforms are critical for evaluation and verification in the research of networking technologies and protocols for space networks(SN).High fidelity emulating technologies have been extensively studied for S...Emulation platforms are critical for evaluation and verification in the research of networking technologies and protocols for space networks(SN).High fidelity emulating technologies have been extensively studied for SN in earlier work,while little emphasis has been placed on the performance evaluation part.In this paper,the design of a network performance analysis architecture is presented,with which high-speed network traffic can be captured and indexed,and the performance of the emulated SN can be well analyzed and evaluated.This architecture comprises three components,namely capture layer,storage layer and analysis layer.Analytic Hierarchy Process(AHP)and several analysis methods are adopted to evaluate the network performance comprehensively.In the implementation of the proposed architecture,configuration optimization and parallel processing are applied to handle large amount of high-speed network traffic.Finally,experiment results through the analysis system exhibits the effectiveness of the proposed architecture.展开更多
Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient meth...Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial trans- port using microfluidic-chamber-cultured neurons together with a newly developed analysis package named ＂MitoQuant＂. This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms （temperature changes, drug treatment and genetic manipulation） that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mltochondrial transport that were not detected by traditional kymographic analyses.展开更多
基金supported by the National Natural Science Foundation of China under Grant 62131012the Fundamental Research Funds for the Central Universities under Grant 021014380187。
文摘Emulation platforms are critical for evaluation and verification in the research of networking technologies and protocols for space networks(SN).High fidelity emulating technologies have been extensively studied for SN in earlier work,while little emphasis has been placed on the performance evaluation part.In this paper,the design of a network performance analysis architecture is presented,with which high-speed network traffic can be captured and indexed,and the performance of the emulated SN can be well analyzed and evaluated.This architecture comprises three components,namely capture layer,storage layer and analysis layer.Analytic Hierarchy Process(AHP)and several analysis methods are adopted to evaluate the network performance comprehensively.In the implementation of the proposed architecture,configuration optimization and parallel processing are applied to handle large amount of high-speed network traffic.Finally,experiment results through the analysis system exhibits the effectiveness of the proposed architecture.
文摘Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial trans- port using microfluidic-chamber-cultured neurons together with a newly developed analysis package named ＂MitoQuant＂. This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms （temperature changes, drug treatment and genetic manipulation） that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mltochondrial transport that were not detected by traditional kymographic analyses.
