Paraffin section observation of flower bud differentiation of Chimonanthus praecox in Kunming and comparison of the differentiation processes in different regions,China

Chimonanthus praecox is an important ornamental plant and cut flower material in China.It blooms in the freezing winter and its flower emits charming fragrance.However,in different region the flowering time is variable.In order to understand the flowering mechanism of Ch.praecox in the winter,we studied the flower bud differentiation in Spring City-Kunming using paraffin sectioning method in the present study.Meanwhile we compared the differentiation process difference from different regions.It was found that the temperature is the key factor for its flower bud differentiation and blossom of Ch.praecox.In the process of bud differentiation,the temperature 20℃was the optimum for inducing changes from vegetative axillary buds to reproductive buds and subsequent morphological differentiation in Ch.praecox.Furthermore in the first three differentiation periods—tepal primordial stage,staminal primordial stage and pistil primordial stage,Kunming took the shortest time to finish the process due to very rapid temperature rise to 20℃,whereas,in Zhengzhou the time for these differentiations was the longest,which may be caused by the slow temperature rise.After May,the high temperature stress forced the flower buds into the first long dormant period in all regions except Kunming.In Kunming,the average temperature was only 20–25℃,so the flower bud continued to differentiate.In all regions,Kunming is the first to complete whole flower bud differentiation even on the early August,and started the second dormancy very early but very long.In the other regions,the plants went through a shorter dormancy and the low temperature broke the dormancy rapidly.Contrarily the plants of Kunming spent a longer period for the low temperature.Thus,the low temperature less than 10℃is a key factor to breaking the second dormancy.Surely the regular effects of temperature on flower bud differentiation and blossom is very helpful for florescence regulation of Ch.praecox.

Sex difference in human diseases:mechanistic insights and clinical implications

Sex characteristics exhibit significant disparities in various human diseases,including prevalent cardiovascular diseases,cancers,metabolic disorders,autoimmune diseases,and neurodegenerative diseases.Risk profiles and pathological manifestations of these diseases exhibit notable variations between sexes.The underlying reasons for these sex disparities encompass multifactorial elements,such as physiology,genetics,and environment.Recent studies have shown that human body systems demonstrate sex-specific gene expression during critical developmental stages and gene editing processes.These genes,differentially expressed based on different sex,may be regulated by androgen or estrogen-responsive elements,thereby influencing the incidence and presentation of cardiovascular,oncological,metabolic,immune,and neurological diseases across sexes.However,despite the existence of sex differences in patients with human diseases,treatment guidelines predominantly rely on male data due to the underrepresentation of women in clinical trials.At present,there exists a substantial knowledge gap concerning sex-specific mechanisms and clinical treatments for diverse diseases.Therefore,this review aims to elucidate the advances of sex differences on human diseases by examining epidemiological factors,pathogenesis,and innovative progress of clinical treatments in accordance with the distinctive risk characteristics of each disease and provide a new theoretical and practical basis for further optimizing individualized treatment and improving patient prognosis.

Primary cilia in hard tissue development and diseases

Bone and teeth are hard tissues.Hard tissue diseases have a serious effect on human survival and quality of life.Primary cilia are protrusions on the surfaces of cells.As antennas,they are distributed on the membrane surfaces of almost all mammalian cell types and participate in the development of organs and the maintenance of homeostasis.Mutations in cilium-related genes result in a variety of developmental and even lethal diseases.Patients with multiple ciliary gene mutations present overt changes in the skeletal system,suggesting that primary cilia are involved in hard tissue development and reconstruction.Furthermore,primary cilia act as sensors of external stimuli and regulate bone homeostasis.Specifically,substances are trafficked through primary cilia by intraflagellar transport,which affects key signaling pathways during hard tissue development.In this review,we summarize the roles of primary cilia in long bone development and remodeling from two perspectives:primary cilia signaling and sensory mechanisms.In addition,the cilium-related diseases of hard tissue and the manifestations of mutant cilia in the skeleton and teeth are described.We believe that all the findings will help with the intervention and treatment of related hard tissue genetic diseases.

Implicit,But Not Explicit,Emotion Regulation Relieves Unpleasant Neural Responses Evoked by High-Intensity Negative Images

Evidence suggests that explicit reappraisal has limited regulatory effects on high-intensity emotions,mainly due to the depletion of cognitive resources occupied by the high-intensity emotional stimulus itself.The implicit form of reappraisal has proved to be resource-saving and therefore might be an ideal strategy to achieve the desired regulatory effect in high-intensity situations.In this study,we explored the regulatory effect of explicit and implicit reappraisal when participants encountered low-and high-intensity negative images.The subjective emotional rating indicated that both explicit and implicit reappraisal down-regulated negative experiences,irrespective of intensity.However,the amplitude of the parietal late positive potential(LPP;a neural index of experienced emotional intensity)showed that only implicit reappraisal had significant regulatory effects in the high-intensity context,though both explicit and implicit reappraisal successfully reduced the emotional neural responses elicited by low-intensity negative images.Meanwhile,implicit reappraisal led to a smaller frontal LPP amplitude(an index of cognitive cost)compared to explicit reappraisal,indicating that the implementation of implicit reappraisal consumes limited cognitive control resources.Furthermore,we found a prolonged effect of implicit emotion regulation introduced by training procedures.Taken together,these findings not only reveal that implicit reappraisal is suitable to relieve high-intensity negative experiences as well as neural responses,but also highlight the potential benefit of trained implicit regulation in clinical populations whose frontal control resources are limited.

Engineering Saccharomyces cerevisiae to produce plant benzylisoquinoline alkaloids

Benzylisoquinoline alkaloids(BIAs)are a diverse family of plant natural products with extensive pharmacological properties,but the yield of BIAs from plant is limited.The understanding of BIA biosynthetic mechanism in plant and the development of synthetic biology enable the possibility to produce BIAs through microbial fermentation,as an alternative to agriculture-based supply chains.In this review,we discussed the engineering strategies to synthesize BIAs in Saccharomyces cerevisiae(yeast)and improve BIA production level,including heterologous pathway reconstruction,enzyme engi neering,expression regulation,host engineering and fermentation engineering.We also highlight recent meta bolic engineering advances in the production of BIAs in yeast.

Recent progress in quantum photonic chips for quantum communication and internet

Recent years have witnessed significant progress in quantum communication and quantum internet with the emerging quantum photonic chips,whose characteristics of scalability,stability,and low cost,flourish and open up new possibilities in miniaturized footprints.Here,we provide an overview of the advances in quantum photonic chips for quantum communication,beginning with a summary of the prevalent photonic integrated fabrication platforms and key components for integrated quantum communication systems.We then discuss a range of quantum communication applications,such as quantum key distribution and quantum teleportation.Finally,the review culminates with a perspective on challenges towards high-performance chip-based quantum communication,as well as a glimpse into future opportunities for integrated quantum networks.

A tumor-penetrable drug nanococktail made from human histones for interventional nucleus-targeted chemophotothermal therapy of drug-resistant tumors

Nanoparticle-based chemophotothermal therapy(CPT)is a promising treatment for multidrug resistant tumors.In this study,a drug nanococktail of DIR825@histone was developed by employing doxorubicin(DOX),NIR dye IR825 and human histones for interventional nucleus-targeted CPT of multidrug resistant tumors with an interventional laser.After localized intervention,DIR825@histone penetrated tumor tissues by transcytosis,efficiently entered tumor cells and targeted the cell nuclei.DIR825@histone also exhibited good photothermal performance and thermal-triggered drug release.Efficient multidrug resistant tumor inhibition was achieved by enhanced CPT sensitization and MDR reversion via nuclear targeting.Moreover,an interventional laser assisted DIR825@histone in inhibiting multidrug resistant tumors by promoting the sufficient delivery of laser energy inside the tumor while reducing skin injury.Therefore,DIR825@histone together with this interventional nucleus-targeted CPT strategy holds great promise for treating multidrug resistant tumors.

^(18)F-FDG PET/MR Imaging for Evaluating of Obstructive Jaundice:Chinese Expert Consensus

The cause of obstructive jaundice is usually complex which renders its differential diagnosis and lesion localization challenging in clinical practice.Integrated Positron Emission tomography/Magnetic Resonance(PET/MR)offers complementary information from PET and MR in the diagnosis of obstructive jaundice and is becoming widely adopted in clinical setting.While preserving its diagnostic accuracy,it is important to standardize and streamline the clinical scan protocol of PET/MR in evaluating obstructive jaundice.Based on literature review and experience of large number of clinical cases from the author group,this article reports an expert consensus on imaging protocol optimization and case interpretation template standardization.