CRISPR/Cas9 systems are an established tool in genome engineering.As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms,new systems were developed that can...CRISPR/Cas9 systems are an established tool in genome engineering.As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms,new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these issues.The recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base exchange.These base editing systems however require additional constraints to be considered for designing the sgRNAs.Here,we present an updated version of the interactive CRISPy-web single guide RNA design tool https://crispy.secondarymetabolites.org/that was built to support“classical”CRISPR and now also CRISPRBEST workflows.展开更多
基金This work was supported by grants from the Novo Nordisk Foundation[NNF10CC1016517,NNF15OC0016226,NNF16OC0021746].
文摘CRISPR/Cas9 systems are an established tool in genome engineering.As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms,new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these issues.The recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base exchange.These base editing systems however require additional constraints to be considered for designing the sgRNAs.Here,we present an updated version of the interactive CRISPy-web single guide RNA design tool https://crispy.secondarymetabolites.org/that was built to support“classical”CRISPR and now also CRISPRBEST workflows.
