CPAL, as a New Mediator of Cardiomyocyte Metabolic Alterations and Pyroptosis, Regulates Myocardial Infarction Injury in Mice

Myocardial infarction (MI), the most serious of the ischemic heart diseases, is accompanied by myocardial metabolic disorders and the loss of cardiomyocytes. Increasing evidence has shown that long noncoding RNAs (lncRNAs) are involved in various pathological conditions such as cancer and cardiovascular diseases (CVDs), and are emerging as a novel biomarker for these disorders. This study aims to investigate the regulatory role and mechanisms of lncRNAs in myocardial remodeling in the setting of MI. We find that post-infarcted hearts exhibit a reduction of adenosine triphosphate (ATP) and an alteration of the glucose and lipid metabolism genes cluster of differentiation 36 (CD36), hexokinase 1 (HK1), and clucose transporter 4 (GLUT4), accompanied by cardiomyocyte pyroptosis. We then identify a previously unknown conserved lncRNA, AK009126 (cardiomyocyte pyroptosis-associated lncRNA, CPAL), which is remarkably upregulated in the myocardial border zone of MI mice. Importantly, the adeno-associated virus 9 (AAV9)-mediated silencing of endogenous CPAL by its short hairpin RNA (shRNA) partially abrogates myocardial metabolic alterations and cardiomyocyte pyroptosis during MI in mice. Mechanistically, CPAL is shown to bind directly to nuclear factor kappa B (NFκB) and to act as an activator of NFκB to induce NFκB phosphorylation in cardiomyocytes. We also find that CPAL upregulates caspase-1 expression at the transcriptional level and consequently promotes the release of interleukin (IL)-18 and IL-1β from cardiomyocytes. Collectively, our findings reveal the conserved lncRNA CPAL as a new regulator of cardiac metabolic abnormalities and cardiomyocyte pyroptosis in the setting of MI and suggest CPAL as a new therapeutic target to protect cardiomyocytes against ischemic injury in infarcted hearts.

Retrospective Analysis of 128 Patients of Acupuncture Consultation After Pancreatic Surgery

This study was conducted to analyze and summaries the current situation of acupuncture post-operative consultation of pancreatic cancer patients in the Qilu hospital of Shandong University,further provide a reference for acupuncture treatment of post-operative complications after pancreatic surgery,especially the effect of acupuncture in treating gastrointestinal dysfunction after pancreatic surgery.The consultation reasons,diagnosis,treatments,patient compliance,and the effect of acupuncture treatment of post-operative was studied and analyzed in 128 cases from October 9,2020 to October 9,2021.A total of 128 patients was selected for this study,and 116 of the patients were completely treated.This study showed that the effective rate of acupuncture;For early post-operative inflammatory bowel obstruction was 94.85%;For post-operative gastric emptying dysfunction was 81.25%;For early post-operative inflammatory bowel obstruction with gastric emptying disorder was 66.67%;and Clinical total effective rate was 91.38%.This study concluded that acupuncture may promote pancreatic gastrointestinal function rehabilitation,and provide a new method to enhance the patient’s recovery after surgery.

Highly efficient electrocatalytic deuteration of acetylene to deuterated ethylene using deuterium oxide

Deuterated ethylene is an important building block for manufacturing various deuterated polyolefins and chemicals.However,low-cost and large-scale production of deuterated ethylene still remain a great challenge.Herein,with D_(2)O as the D source,we first propose an electrocatalytic deuteration strategy for continuous production of deuterated ethylene from acetylene under ambient conditions.Specially,Ag nanoparticles exhibit a very high deuterated ethylene Faradic efficiency of up to 99.3%at-0.6 V vs.reversible hydrogen electrode.Meanwhile,Ag nanoparticles achieve a deuterated ethylene production rate of 3.72×10^(3)mmol h^(-1)g^(-1)cat and an excellent long-term stability with deuterated ethylene Faradaic efficiencies of~95%in a two-electrode flow cell,which substantially outperform state-of-the-art values for previously reported deuterated alkenes.In-situ electrochemical Infrared absorption and Raman spectroscopies reveal superior acetylene absorption and formation of deuterated ethylene on Ag nanoparticles.This efficient electrocatalytic deuteration strategy opens a new window for continuous and economic production of deuterated alkenes.