One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were conf...One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were confirmed positive for the presence of this pathogen. Stapylococcus aureus isolates identified appeared typically and atypically characteristics of growth colonies in Baird Parker agar plates at 37°C for 24-48 h. 6 out of 19 strains showed typical characteristics and 13 out 19 (68%) isolates had atypical characteristics. Coagulase positive Staphylococcus aureus was found in 18% (1911 05) of fresh milk samples. Milk produced by cows with subclinical mastitis largely influences Staphylococcus aureus count of bulk tank milk than contamination of milking and milk handling equipment by this pathogen. All S. aureus isolates were coagulase positive. 5 (or about 25%) of isolates of S. aureus identified were resistant to penicillin, 7 out 19 isolates or 36% resistant to methicilin and 9 out 19 strains or 47% resistant to vancomycin. Another objective of this study was the evaluation of the number of S. aureus per mL raw milk. The results confirmed that II (57.8%) out of 19 positive cases had more than 100 cfu/mL, indicating a possible risk for intoxication caused by production of entertoxines of coagulase positive S. aureus.展开更多
文摘One hundred and five raw cow milk samples were collected from cattle farms located in Fieri from 2007 to 2009 and have been analyzed for presence of coagulase positive Staphylococcus aureus. Nineteen samples were confirmed positive for the presence of this pathogen. Stapylococcus aureus isolates identified appeared typically and atypically characteristics of growth colonies in Baird Parker agar plates at 37°C for 24-48 h. 6 out of 19 strains showed typical characteristics and 13 out 19 (68%) isolates had atypical characteristics. Coagulase positive Staphylococcus aureus was found in 18% (1911 05) of fresh milk samples. Milk produced by cows with subclinical mastitis largely influences Staphylococcus aureus count of bulk tank milk than contamination of milking and milk handling equipment by this pathogen. All S. aureus isolates were coagulase positive. 5 (or about 25%) of isolates of S. aureus identified were resistant to penicillin, 7 out 19 isolates or 36% resistant to methicilin and 9 out 19 strains or 47% resistant to vancomycin. Another objective of this study was the evaluation of the number of S. aureus per mL raw milk. The results confirmed that II (57.8%) out of 19 positive cases had more than 100 cfu/mL, indicating a possible risk for intoxication caused by production of entertoxines of coagulase positive S. aureus.