Siderophores,being iron-chelators,have received much attention in environmental research,medicine,and biotechnology because of their high affinity and specificity to a wide range of metals.This study aimed to investig...Siderophores,being iron-chelators,have received much attention in environmental research,medicine,and biotechnology because of their high affinity and specificity to a wide range of metals.This study aimed to investigate siderophore production of culturable soil fungi from Mt.Yangbew,Tawang,La Trinidad,Benguet and to determine their chemical characteristics.Siderophore production was detected and quantified using Chrome Azurol Sulphonate assay while the siderophore characterization was through FeCl_(3),Csáky’s and Arnow’s tests.It was found that 11 out of 20 pure cultures,which showed positive CAS assay results,produce hydroxamates.ITS primers were used in PCR amplification for the molecular identification of the top-four siderophore-producers.Based on BLASTn analyses of their sequences,the isolates were found to be most homologous to species of Talaromyces,Byssochlamys,Paecilomyces,and Penicillium.The identified species were subjected to phylogenetic analysis using MEGA 7 to the show evolutionary history and relationship among the fungal species.展开更多
基金the support of the Department of Biology,College of Science,University of the Philippines Baguio for the conduct of this study.
文摘Siderophores,being iron-chelators,have received much attention in environmental research,medicine,and biotechnology because of their high affinity and specificity to a wide range of metals.This study aimed to investigate siderophore production of culturable soil fungi from Mt.Yangbew,Tawang,La Trinidad,Benguet and to determine their chemical characteristics.Siderophore production was detected and quantified using Chrome Azurol Sulphonate assay while the siderophore characterization was through FeCl_(3),Csáky’s and Arnow’s tests.It was found that 11 out of 20 pure cultures,which showed positive CAS assay results,produce hydroxamates.ITS primers were used in PCR amplification for the molecular identification of the top-four siderophore-producers.Based on BLASTn analyses of their sequences,the isolates were found to be most homologous to species of Talaromyces,Byssochlamys,Paecilomyces,and Penicillium.The identified species were subjected to phylogenetic analysis using MEGA 7 to the show evolutionary history and relationship among the fungal species.
