Vibrio splendidus is an important opportunistic pathogen ubiquitously present in the marine environment,exhibiting virulence to a variety of cultured animals.The extracellular products secreted by V.splendidus are cru...Vibrio splendidus is an important opportunistic pathogen ubiquitously present in the marine environment,exhibiting virulence to a variety of cultured animals.The extracellular products secreted by V.splendidus are crucial to bacterial survival and virulence.In this study,the secretion of outer membrane vesicles(OMVs)by V.splendidus was determined,purified,and morphologically characterized.The protein composition of OMVs was analyzed by proteomic analysis.The results showed that approximately 120 proteins were contained in these OMVs,including outer membrane proteins,flagellins,ABC transporters,protease,and iron regulation proteins,etc.,which were involved in bacterial motility,formation of biofilms and the cell membrane components,and cellular localization based on their structural molecule activity,passive transmembrane transporter activity,channel activity,neurotransmitter receptor activity,extracellular ligand-gated ion channel activity,glutamate receptor activity,ligand-gated ion channel activity,and transmembrane signaling receptor activity.To explore the biological functions of OMVs in V.splendidus,the effects of OMVs on the bacterial adaption to iron limitation,antibiotic,and the coelomic fluid of the Apostichopus japonicus were confirmed.This study is the first time to show that V.splendidus secretes OMVs,and OMVs carry functional proteins that enhance bacterial survival under various stresses.展开更多
目的:系统评价太极拳对轻度认知障碍老年人认知功能及心理状态的影响。方法:通过计算机检索PubMed、Embase、CENTRAL、CINAHL、Web of Science、CNKI、VIP、Wanfang Data和CBM数据库,收集轻度认知障碍老年人太极拳干预的随机对照试验。...目的:系统评价太极拳对轻度认知障碍老年人认知功能及心理状态的影响。方法:通过计算机检索PubMed、Embase、CENTRAL、CINAHL、Web of Science、CNKI、VIP、Wanfang Data和CBM数据库,收集轻度认知障碍老年人太极拳干预的随机对照试验。采用RevMan 5.3软件进行Meta分析。结果:共纳入7项随机对照试验。Meta分析结果显示,与普通运动或者常规护理措施相比,太极拳可以改善老年人的记忆力[MD=0.37,95%CI(0.13,0.61),P<0.01]及视空间功能[SMD=0.57,95%CI(0.23,0.91),P<0.01]。结论:太极拳可以改善轻度认知功能障碍老年人记忆力、视空间功能,但对整体认知功能、执行能力、语言流畅性及抑郁情绪等指标无明显改善。展开更多
基金the Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars(No.LR20C190001)the National Natural Science Foundation of China(No.31972833)+1 种基金the Fundamental Research Funds for the Provincial Universities of Zhejiang(No.SJ LZ2020001)the K.C.Wong Magna Fund at Ningbo University。
文摘Vibrio splendidus is an important opportunistic pathogen ubiquitously present in the marine environment,exhibiting virulence to a variety of cultured animals.The extracellular products secreted by V.splendidus are crucial to bacterial survival and virulence.In this study,the secretion of outer membrane vesicles(OMVs)by V.splendidus was determined,purified,and morphologically characterized.The protein composition of OMVs was analyzed by proteomic analysis.The results showed that approximately 120 proteins were contained in these OMVs,including outer membrane proteins,flagellins,ABC transporters,protease,and iron regulation proteins,etc.,which were involved in bacterial motility,formation of biofilms and the cell membrane components,and cellular localization based on their structural molecule activity,passive transmembrane transporter activity,channel activity,neurotransmitter receptor activity,extracellular ligand-gated ion channel activity,glutamate receptor activity,ligand-gated ion channel activity,and transmembrane signaling receptor activity.To explore the biological functions of OMVs in V.splendidus,the effects of OMVs on the bacterial adaption to iron limitation,antibiotic,and the coelomic fluid of the Apostichopus japonicus were confirmed.This study is the first time to show that V.splendidus secretes OMVs,and OMVs carry functional proteins that enhance bacterial survival under various stresses.
文摘目的:系统评价太极拳对轻度认知障碍老年人认知功能及心理状态的影响。方法:通过计算机检索PubMed、Embase、CENTRAL、CINAHL、Web of Science、CNKI、VIP、Wanfang Data和CBM数据库,收集轻度认知障碍老年人太极拳干预的随机对照试验。采用RevMan 5.3软件进行Meta分析。结果:共纳入7项随机对照试验。Meta分析结果显示,与普通运动或者常规护理措施相比,太极拳可以改善老年人的记忆力[MD=0.37,95%CI(0.13,0.61),P<0.01]及视空间功能[SMD=0.57,95%CI(0.23,0.91),P<0.01]。结论:太极拳可以改善轻度认知功能障碍老年人记忆力、视空间功能,但对整体认知功能、执行能力、语言流畅性及抑郁情绪等指标无明显改善。
