CORM-3, a water soluble transitional metal carbonyls based around ruthenium can release CO activated bylight[1]. Although CORM-3 has resulted in promising preclinical data, such as anti-inflammatory, anti-apoptosisand...CORM-3, a water soluble transitional metal carbonyls based around ruthenium can release CO activated bylight[1]. Although CORM-3 has resulted in promising preclinical data, such as anti-inflammatory, anti-apoptosisand vasodilatory effect, they present a poorly understanding on toxicological profile.To evaluate the effects of CORM-3 on the developmentof zebrafish embryos, we selected the zebrafish embryosdeveloping normally at the stage of blastocyst at4 h post fertilization and transferred into 24-well cultureplates with 2 embryos per well and then exposedto freshly prepared CORM-3 solution with E3 (0, 6.25,12.5, 25, 50, 100, 200 and 400 mol/L) for 4144 hpffor a continuing observation period on the developmentof zebrafish. Acute endpoints including hatching rateand embryonic/larval mortality were evaluated every 24h(Fig. 1).展开更多
基金Key Program of National Natural Science Foundation of China (U1432248), National Natural Science Foundationof China (11305226, 11175222).
文摘CORM-3, a water soluble transitional metal carbonyls based around ruthenium can release CO activated bylight[1]. Although CORM-3 has resulted in promising preclinical data, such as anti-inflammatory, anti-apoptosisand vasodilatory effect, they present a poorly understanding on toxicological profile.To evaluate the effects of CORM-3 on the developmentof zebrafish embryos, we selected the zebrafish embryosdeveloping normally at the stage of blastocyst at4 h post fertilization and transferred into 24-well cultureplates with 2 embryos per well and then exposedto freshly prepared CORM-3 solution with E3 (0, 6.25,12.5, 25, 50, 100, 200 and 400 mol/L) for 4144 hpffor a continuing observation period on the developmentof zebrafish. Acute endpoints including hatching rateand embryonic/larval mortality were evaluated every 24h(Fig. 1).
