.Hypoxia precondition plays a protective role in tissues injured by oxidative stress.Whether or not mitophagy is involved needs to be explored.In the present study,the acute kidney injury model was established by foli....Hypoxia precondition plays a protective role in tissues injured by oxidative stress.Whether or not mitophagy is involved needs to be explored.In the present study,the acute kidney injury model was established by folic acid(FA)administration.Roxadustat(FG-4592),a stabilizer of hypoxia-inducible factor(HIF)was used 2 days before FA insult to mimic hypoxia precondition.First of all,the results showed a protective effect,in both renal function and morphology with FG-4592 pretreatment.In addition,BNIP3 and FUNDC1,two key players in.receptor-mediated mitophagy,were induced to express with upregulation of HIF-1alpha.Meanwhile the mitochondrial marker translocase of outer mitochondrial membrane 20(TOMM20)and mtDNA decreased,indicating a decline of mitochondrial quality.On the other hand,PGC-lalpha,the master regulator of mitochondrial biogenesis,was detected no statistical significance,which implied that the decline may attribute to more mitophagy rather than less biogenesis.That upregulation of LC3B and downregulation of p62/SQSTM1 further proved the presence of mitophagy.展开更多
The effective filtration area is determined by the length of capillaries in the glomerulus,however,a quantitative morphological data describing it is not available.Present study aims a quantitatively describing the de...The effective filtration area is determined by the length of capillaries in the glomerulus,however,a quantitative morphological data describing it is not available.Present study aims a quantitatively describing the development of the capillary loop stage glomerulus(capG)with respect to the volume density of the glomerular ca pillary endothelial cells based on the kidney morphogenesis.The kidneys were obtained from mice at various developing time points and prepared for paraf fin sections.The volume density of CD34 positive endothelial cells in glomeruli was measured using immunohistochemical staining and stereological grid system.The capG was divided into early,early-middle,late-middle,and late phases,according to the morphology of developing glomeruli and the arrangement of podocytes.展开更多
目的探讨不同程度胎盘植入剖宫产手术麻醉方式的选择及母婴结局。方法收集2018年至2021年在广州医科大学附属第三医院诊断为"胎盘植入"的562例患者的临床资料,排除剖宫取胎、人工流产、数据缺失等病例,共纳入353例产妇;根据...目的探讨不同程度胎盘植入剖宫产手术麻醉方式的选择及母婴结局。方法收集2018年至2021年在广州医科大学附属第三医院诊断为"胎盘植入"的562例患者的临床资料,排除剖宫取胎、人工流产、数据缺失等病例,共纳入353例产妇;根据磁共振成像影像检查的胎盘植入程度将产妇分成三组:粘连组(63例),植入组(249例),穿透组(41例);分析三组产妇的术前一般情况、麻醉方式选择、术中管理情况及母婴结局。结果三组产妇中,粘连组有27例(42.9%)选择腰硬联合麻醉,而穿透组仅2例(4.9%);穿透组有39例(95.1%)选择全身麻醉,占比高于粘连组(57.1%)和植入组(81.5%),三组麻醉方式选择差异有统计学意义(χ^(2)=24.82,P<0.05)。穿透组36例(87.8%)术中需行子宫切除术,明显高于粘连组(38.1%)和植入组(57.4%),差异有统计学意义(P<0.05)。穿透组的新生儿1 min Apgar评分(17.33±1.57)低于粘连组(8.64±1.70)和植入组(7.77±1.84),差异有统计学意义(F=9.35,P<0.05),而三组的新生儿5 min、10 min Apgar评分差异无统计学意义(P>0.05)。结论麻醉医师应根据术前影像学提示的植入程度选择麻醉方式,植入程度越重,越倾向选择全身麻醉。术中经过产科医师、麻醉医师及新生儿科医师合作,母婴预后结局良好,但穿透性胎盘植入患者子宫切除率高。展开更多
文摘.Hypoxia precondition plays a protective role in tissues injured by oxidative stress.Whether or not mitophagy is involved needs to be explored.In the present study,the acute kidney injury model was established by folic acid(FA)administration.Roxadustat(FG-4592),a stabilizer of hypoxia-inducible factor(HIF)was used 2 days before FA insult to mimic hypoxia precondition.First of all,the results showed a protective effect,in both renal function and morphology with FG-4592 pretreatment.In addition,BNIP3 and FUNDC1,two key players in.receptor-mediated mitophagy,were induced to express with upregulation of HIF-1alpha.Meanwhile the mitochondrial marker translocase of outer mitochondrial membrane 20(TOMM20)and mtDNA decreased,indicating a decline of mitochondrial quality.On the other hand,PGC-lalpha,the master regulator of mitochondrial biogenesis,was detected no statistical significance,which implied that the decline may attribute to more mitophagy rather than less biogenesis.That upregulation of LC3B and downregulation of p62/SQSTM1 further proved the presence of mitophagy.
文摘The effective filtration area is determined by the length of capillaries in the glomerulus,however,a quantitative morphological data describing it is not available.Present study aims a quantitatively describing the development of the capillary loop stage glomerulus(capG)with respect to the volume density of the glomerular ca pillary endothelial cells based on the kidney morphogenesis.The kidneys were obtained from mice at various developing time points and prepared for paraf fin sections.The volume density of CD34 positive endothelial cells in glomeruli was measured using immunohistochemical staining and stereological grid system.The capG was divided into early,early-middle,late-middle,and late phases,according to the morphology of developing glomeruli and the arrangement of podocytes.
文摘目的探讨不同程度胎盘植入剖宫产手术麻醉方式的选择及母婴结局。方法收集2018年至2021年在广州医科大学附属第三医院诊断为"胎盘植入"的562例患者的临床资料,排除剖宫取胎、人工流产、数据缺失等病例,共纳入353例产妇;根据磁共振成像影像检查的胎盘植入程度将产妇分成三组:粘连组(63例),植入组(249例),穿透组(41例);分析三组产妇的术前一般情况、麻醉方式选择、术中管理情况及母婴结局。结果三组产妇中,粘连组有27例(42.9%)选择腰硬联合麻醉,而穿透组仅2例(4.9%);穿透组有39例(95.1%)选择全身麻醉,占比高于粘连组(57.1%)和植入组(81.5%),三组麻醉方式选择差异有统计学意义(χ^(2)=24.82,P<0.05)。穿透组36例(87.8%)术中需行子宫切除术,明显高于粘连组(38.1%)和植入组(57.4%),差异有统计学意义(P<0.05)。穿透组的新生儿1 min Apgar评分(17.33±1.57)低于粘连组(8.64±1.70)和植入组(7.77±1.84),差异有统计学意义(F=9.35,P<0.05),而三组的新生儿5 min、10 min Apgar评分差异无统计学意义(P>0.05)。结论麻醉医师应根据术前影像学提示的植入程度选择麻醉方式,植入程度越重,越倾向选择全身麻醉。术中经过产科医师、麻醉医师及新生儿科医师合作,母婴预后结局良好,但穿透性胎盘植入患者子宫切除率高。
