Could extracellular vesicles derived from mesenchymal stem cells be a potential therapy for acute pancreatitis-induced cardiac injury?

Acute pancreatitis(AP)often leads to a high incidence of cardiac injury,posing significant challenges in the treatment of severe AP and contributing to increased mortality rates.Mesenchymal stem cells(MSCs)release bioactive molecules that participate in various inflammatory diseases.Similarly,extracellular vesicles(EVs)secreted by MSCs have garnered extensive attention due to their comparable anti-inflammatory effects to MSCs and their potential to avoid risks associated with cell transplantation.Recently,the therapeutic potential of MSCs-EVs in various inflammatory diseases,including sepsis and AP,has gained increasing recognition.Although preclinical research on the utilization of MSCs-EVs in AP-induced cardiac injury is limited,several studies have demonstrated the positive effects of MSCs-EVs in regulating inflammation and immunity in sepsis-induced cardiac injury and cardiovascular diseases.Furthermore,clinical studies have been conducted on the therapeutic application of MSCs-EVs for some other diseases,wherein the contents of these EVs could be deliberately modified through prior modulation of MSCs.Consequently,we hypothesize that MSCs-EVs hold promise as a potential therapy for AP-induced cardiac injury.This paper aims to discuss this topic.However,additional research is essential to comprehensively elucidate the underlying mechanisms of MSCs-EVs in treating AP-induced cardiac injury,as well as to ascertain their safety and efficacy.

关于网络异常信息数据快速提取仿真

对网络异常信息数据进行提取,在提高网络运行稳定性及有效性方面具有重要意义。由于网络异常信息数据的易变性及易干扰性,使得异常信息数据特征不稳定;传统方法在对异常信息数据进行提取时,针对特征不稳定性,引入异常信息数据判断规则,确定网络不同异常信息数据特征之间的关联规则,完成异常信息数据的提取,但忽略了异常信息数据判断规则的生成过程,对异常数据特征带来的干扰,导致提取精度偏低。提出基于KNN思想重新定义的网络异常信息数据快速提取方法。在分析网络异常信息数据系统原理特征基础上,获取异常信息数据特征响应函数,以平方差函数值最小为机制更新簇的中心点,求得异常信息数据的功率谱密度函数作为数据特征,结合遗传算法进行异常信息数据特征优选。采用KNN思想重新定义异常信息数据局部密度和距离,考虑异常信息数据的优选特征,给出异常信息数据判断规则,实现异常信息数据快速提取。实验结果表明,该方法异常信息数据提取覆盖率较高,且有效降低了内存开销和时间开销。

Changes in count and function of splenic lymphocytes from patients with portal hypertension

AIM: To investigate changes in numbers and proliferative function of splenic lymphocytes in patients with hypersplenism due to portal hypertension (PH), to provide evidence for further study of immune status of the spleen during PH. METHODS: Twelve spleens from patients with hypersplenism due to PH served as the PH group, and four spleens from cases of traumatic spleen rupture were regarded as the control group. After weighing the spleen, lymphocytes were separated and counted using a cell counting plate to calculate the lymphocyte count per gram of spleen tissue (relative quantity) and total lymphocyte count in whole spleen (absolute quantity). The immunohistochemical SP method was used to observe the density and distribution of lymphocytes in the spleen. The MTT method was used to observe changes in lymphocyte proliferative function. RESULTS: As compared to the control group, the splenic lymphocytes in the PH group showed that: (1) There was no difference in distribution but a significant decreasein density; (2) the number of lymphocytes per gram of spleen (relative quantity) decreased significantly (0.822 ± 0.157) × 108 vs (1.174 ± 0.254) × 108, P < 0.01]; (3) with the significant increase in the weight of the PH spleen (832.6 ± 278.2 g vs 211.7 ± 85.6 g, P < 0.01), the total quantity of lymphocytes (absolute quantity) increased significantly (0.685 ± 0.072) × 1011 vs (0.366 ± 0.057) × 1011, P < 0.01]; and (4) the proliferative function of lymphocytes was enhanced: T lymphocytes, (0.022 ± 0.005 vs 0.015 ± 0.003, P < 0.05), and B lymphocytes (0.034 ± 0.006 vs 0.023 ± 0.001, P < 0.01). CONCLUSION: Although lymphocyte density in the spleen decreased in patients with PH, the total quantity of lymphocytes increased because spleen weight increased greatly, along with the proliferating function. With respect to changes in lymphocytes, PH spleens may still have immune function, although it may be disordered. However, complete evaluation of the immune function of the spleen in PH requires more research.

P2Y1R is involved in visceral hypersensitivity in rats with experimental irritable bowel syndrome

AIM To evaluate the role of P2Y1 R in visceral hypersensitivity in rats with experimental irritable bowel syndrome.METHODS A rat model of irritable bowel syndrome was generated by intra-colonic administration of acetic acid(AA) and assessed by histology and myeloperoxidase(m PO) activity assay. Then P2Y1 R expression in the colonic tissue was detected by Western blot. In order to explore the regulatory role of P2Y1 R in visceral hypersensitivity, an agonist(m RS2365) and an antagonist(m RS2179) of P2Y1 R were intra-colonically administered and effects were tested through a colorectal distension test. The abdominal withdrawal reflex and abdominal electromyography were tested during the course. RESULTS model assessment tests showed an obvious inflammatoryreaction that appeared on the 2^(nd) d after the AA injection, and the inflammatory reaction gradually recovered and almost disappeared on the 7^(th) d. The model finished on day 8 and showed a clear feature of IBS that had no organic lesion. The average expression of P2Y1 R was significantly higher in the AA group than in the na?ve group(0.319 ± 0.02 vs 0.094 ± 0.016, P < 0.001). m RS2365 could effectively raise the colonic hypersensitivity status at intervention doses of 10(AUC value from 0.30 ± 0.089 to 1.973 ± 0.127 mv?s, P < 0.01) and 100 μmol/L(AUC value from 0.290 ± 0.079 to 1.983 ± 0.195 mv?s, P < 0.01); m RS2179 could effectively reduce the hypersensitivity status at intervention dose of 100 μmol/L(from a mean baseline AUC value of 1.587 ± 0.099 mv?s to 0.140 ± 0.089 mv?s, P < 0.0001). Differences between the m RS2179 group(1.88 ± 1.45) and either the m RS2365 group(3.96 ± 0.19) or the combined treatment(m RS2179 and m RS2365) group(3.28 ± 0.11) were significant(P < 0.01).CONCLUSION P2Y1 R plays a regulatory role in visceral hypersensitivity in rats with experimental IBS. Specific antagonists of P2Y1 R may have potential therapeutic value in treating abdominal pain in IBS.

Self-recovery ability of stress-damaged salt rock experiment

After being compressed to different plastic deformation stages, the salt rock samples with lateral stress damage of 0.2, 0.3, 0.4, and 0.5 were selected. Ultrasonic technology was used to monitor the wave velocity variation law of stress-dam-aged salt rock during the self-recovery experiment under different temperatures to analyze the influence of initial stress damage and temperature during the self-recovery of salt rock. The experiment shows that the change of salt rock axial wave velocity is smaller than that of lateral wave velocity. The sample ultrasonic velocity is positively correlated with the time of self-recovery, and the damage had been recovered to a certain extent. In the first 200 hours of self-recovery stage, the salt rock lateral damage recovers fast, and then the damage remains almost unchanged. The value of lateral stable damage is positively correlated with the value of lateral initial stress damage. With the increase of temperature, the recovery of lateral damage speeds up and the value of stable damage decreases; the axial damage of salt rock almost remains unchanged during the self-recovery experiment.

激光位移传感器在轨道交通桥梁监测中的应用

位移监测是轨道交通桥梁监测的重要内容之一。为避免接触式位移传感器存在的精度低、易磨损、长期稳定性差等缺点,选用激光位移传感器用于桥梁梁体、支座位移和结构微裂缝的测量,并简要介绍激光位移传感器的工作原理、选型设计、现场安装方法、应用实例和运行维护经验。

乳果糖联合复方聚乙二醇电解质散在超重肥胖结肠镜肠道准备中的应用效果

[目的]探讨乳果糖联合复方聚乙二醇电解质散在超重肥胖结肠镜检查前肠道准备中疗效及安全性。[方法]选取拟行结肠镜检查的176例超重肥胖患者作为研究对象,随机分为观察组(86例)、对照组(90例)。观察组采用乳果糖联合2 L复方聚乙二醇电解质散;对照组单用3 L复方聚乙二醇电解质散。比较2组患者的肠道清洁度、息肉检出率及不良反应总发生率;2组均由内镜医师单盲完成,患者对不同肠道准备方案的耐受性和不良反应采用问卷调查的方式获得。[结果]所有患者均完成肠道准备及结肠镜检查;观察组与对照组Boston肠道准备量表(BBPS)评分分别7.8±1.1、7.9±0.9,差异无统计学意义(P>0.05);观察组、对照组不良反应总发生率分别10.4%(9/86)、20.0%(18/90),差异有统计学意义(P<0.05);观察组和对照组息肉检出率分别41.8%(36/86)、43.3%(39/90),差异无统计学意义(P>0.05)。[结论]乳果糖联合2 L复方聚乙二醇电解质散的肠道准备方案与单用3 L复方聚乙二醇电解质散的肠道准备效果相当,联合乳果糖可减少口服复方聚乙二醇电解质散泻剂容量,降低肠道准备过程中的不良反应总发生率。