Genome-wide SNP markers provided insights into the reproductive strategy and genetic diversity of the green tide causative species Ulva prolifera in China

Ulva prolifera is the causative species of the annually occurring large-scale green tides in China since 2007.Its specific biological features on reproductivity strategies,as well as intra-species genetic diversity,are still largely unknown,especially at the genome level,despite their importance in understanding the formation and outbreak of massive green tides.In the present study,the restriction site-associated DNA genotyping approach(2b-RAD)was adopted to identify the genome-wide single-nucleotide polymorphisms(SNPs)of 54 individual thalli including samples collected from Subei Shoal in 2019 and Qingdao coast from 2019 to 2021.SNPs genotype results revealed that most of the thalli in 2019 and 2020 were haploid gametophytes,while only half of the thalli were gametophytes in 2021,indicating flexibility in the reproductive strategies for the formation of the green tides among different years and the dominance of asexual and vegetative reproductive mode for the floating period.Besides,population analysis was conducted,and it revealed a very low genetic diversity among samples from Subei Shoal and the Qingdao coast in the same year and a higher divergence among samples in different years.The results showed the efficiency of 2b-RAD in the exploration of SNPs in U.prolifera and provided the first genome-wide scale evidence for the origin of the large-scale green tides on the Qingdao coast.This study improved our understanding of the reproductive strategy and genetic diversity of the green tide causative species and will help further reveal the biological causes of the green tide in China.

Photocatalytic degradation of methylene blue over MIL-100(Fe)/GO composites: a performance and kinetic study

Adsorption coupled with photocatalytic degradation is proposed to fulfill the removal and thorough elimination of organic dyes.Herein,we report a facile hydrothermal synthesis of MIL-100(Fe)/GO photocatalysts.The adsorption and photocatalytic degradation process of methylene blue(MB)on MIL‐100(Fe)/GO composites were systematically studied from performance and kinetic perspectives.A possible adsorption‐photocatalytic degradation mechanism is proposed.The optimized 1M8G composite achieves 95%MB removal(60.8 mg/g)in 210 min and displays well recyclability over ten cycles.The obtained MB adsorption and degradation results are well fitted onto Langmuir isotherm and pseudo‐second order kinetic model.This study shed light on the design of MOFs based composites for water treatment.

Experimental clearance rate and intraguild predation of jellyfish Cyanea nozakii

Cyanea nozakii,a common jellyfish distributed in offshore China,has a complex trophic relationship with other zooplankton groups.However,few studies have reported the predation rates and prey selection patterns of C.nozakii medusae on different prey items.Research is also lacking on the intraguild predation of Aurelia coerulea(another common bloom jellyfish in offshore China)by C.nozakii.To address the knowledge gaps,the clearance rates of C.nozakii for different prey items,including copepods(small<1000μm and large>1000μm),fish larvae,and gelatinous prey(hydromedusae,A.coerulea ephyrae,and chaetognaths),were measured.The influence of predator size on the clearance rate was also determined.Additionally,we examined the intraguild predation of C.nozakii on A.coerulea medusae.The clearance rates of C.nozakii varied widely with prey organisms,being independent of prey concentrations.Gelatinous organisms,except for chaetognaths,were captured with considerably high efficiency,followed by fish larvae and copepods,indicating the preferential prey selection of gelatinous organisms by C.nozakii.The clearance rate increased linearly with the cross-sectional area of C.nozakii.Body size in medusae may,to some extents,underpin their capacity to capture more prey by increasing the encounter rate and capture success through ontogeny.C.nozakii preyed voraciously on A.coerulea in high feeding efficiency,but the clearance rate decreased with increasing A.coerulea(as prey)size.This phenomenon of intraguild predation suggests a speculative hypothesis of potential population regulation of A.coerulea by C.nozakii.The information regarding the feeding ecology of C.nozakii reported in this study is important for understanding plankton dynamics in marine ecosystems with extensive occurrences of this jellyfish.

Spatial patterns of zooplankton abundance,biovolume,and size structure in response to environmental variables:a case study in the Yellow Sea and East China Sea

The Yellow Sea(YS)and East China Sea(ECS)are highly dynamic marginal seas of the northwestern Pacific Ocean.To gain an in-depth understanding of zooplankton community structure,zooplankton abundance,biovolume,and size structure in summer 2017 in the YS and ECS were assessed using ZooScan imaging analysis.Zooplankton abundance and biovolume ranged 2.94–1187.14 inds./m^(3)and 3.13–3438.51 mm^(3)/m^(3),respectively.Based on the biovolume data of the categorized size classes of 26 identified taxonomic groups,the zooplankton community was classified into five groups,and each group was coupled with distinctive oceanographic features.Under the influence of the Yellow Sea Cold Water Mass,the Yellow Sea offshore group featured the lowest bottom temperature(10.84±3.42℃)and the most abundant Calanoids(mainly in the 2–3 mm size class).In the Yellow Sea inshore group,Hydrozoans showed the largest biovolume and dominated in the 3–4-mm and>5-mm size classes.The East China Sea offshore group,which was affected by the Kuroshio Branch Current,featured high temperature and salinity,and the lowest bottom dissolved oxygen(2.58±0.5 mg/L).The lowest values of zooplankton abundance and biovolume in the East China Sea offshore group might be attributed to the bottom dissolved oxygen contents.The East China Sea inshore group,which was mainly influenced by the Zhejiang-Fujian Coastal Current and Changjiang Diluted Water,was characterized by high chlorophyll a and the largest biovolume of carnivorous Siphonophores(280.82±303.37 mm^(3)/m^(3)).The Changjiang River estuary offshore group showed the most abundant Cyclopoids,which might be associated with the less turbid water mass in this region.Seawater temperature was considered the most important factor in shaping the size compositions of Calanoids in different groups.

Distribution,abundance,and realized niches of meroplankton by two different mesh size nets during spring 2017 in the Southern Yellow Sea,China

Meroplankton play a crucial role in both benthic and pelagic ecosystems.Existing quantitative research on estimating the quantities of meroplankton groups is both underrepresented and inaccurate.To investigate and evaluate the influence of varying mesh sizes(505 and 160μm)on the sampling efficiency of meroplankton,we conducted an examination using two commonly used plankton nets during the spring season in the Southern Yellow Sea(SYS).Our study revealed a total of 12 meroplankton groups,with 9 groups identified in the 505-μm mesh nets and 11 groups in the 160-μm mesh nets.The results demonstrated the superior collection efficiency of the 160-μm net compared to the 505-μm net across the majority of meroplankton groups.Furthermore,we focused on exploring the abundance,distribution patterns,and realized niches of meroplankton collected by the two mesh size nets,and observed that the distribution of meroplankton closely resembled the distribution of possible benthic adults in the SYS.Correlation analysis of the six dominant groups collected in the 160-μm mesh nets revealed that seawater temperature and salinity emerged as the key environmental factors driving variations in meroplankton abundance within the SYS.This study also found that a smaller mesh size net does not necessarily capture meroplankton more comprehensively.A comprehensive understanding of the ecological characteristics of meroplankton requires the combination of two types of nets for research.Our research significantly advances our understanding of the quantification,abundance,and distribution of meroplankton,serving as a valuable contribution to the broader landscape of detailed quantitative meroplankton studies.

Parent-offspring relationship recognition based on SSR and mtDNA confirmed resource supplement effect of Fenneropenaeus chinensis release

The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the relevant authorities have carried out the activities of stock enhancement and releasing.It can increase biomass and recover resources.However,compared with increasing biomass,there were still few reports on its effect on the recovery of resources.Resource recovery is a process related to whether the released individuals can form a reproductive population.Up to now,there has been a lack of evidence whether the released F.chinensis can complete the entire life history,and form reproduction population.In this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow Sea.After identifying parentage relationships using simple sequence repeat(SSR)and mtDNA haplotype,it was finally confirmed that there were eight released individuals in the recapture samples.It was confirmed for the first time that at least part of the released F.chinensis can complete overwintering and reproductive migration,and maintain the migration habits as their wild counterparts.Therefore,we infered that the released shrimp can reproduce under natural conditions,these F.chinensis can form reproductive populations theoretically if without human intervention.These results indicated that enhancenment and release activities have a positive effect on resource recovery.

Transformer-Based Cloud Detection Method for High-Resolution Remote Sensing Imagery

Cloud detection from satellite and drone imagery is crucial for applications such as weather forecasting and environmentalmonitoring.Addressing the limitations of conventional convolutional neural networks,we propose an innovative transformer-based method.This method leverages transformers,which are adept at processing data sequences,to enhance cloud detection accuracy.Additionally,we introduce a Cyclic Refinement Architecture that improves the resolution and quality of feature extraction,thereby aiding in the retention of critical details often lost during cloud detection.Our extensive experimental validation shows that our approach significantly outperforms established models,excelling in high-resolution feature extraction and precise cloud segmentation.By integrating Positional Visual Transformers(PVT)with this architecture,our method advances high-resolution feature delineation and segmentation accuracy.Ultimately,our research offers a novel perspective for surmounting traditional challenges in cloud detection and contributes to the advancement of precise and dependable image analysis across various domains.

A Security Trade-Off Scheme of Anomaly Detection System in IoT to Defend against Data-Tampering Attacks

Internet of Things(IoT)is vulnerable to data-tampering(DT)attacks.Due to resource limitations,many anomaly detection systems(ADSs)for IoT have high false positive rates when detecting DT attacks.This leads to the misreporting of normal data,which will impact the normal operation of IoT.To mitigate the impact caused by the high false positive rate of ADS,this paper proposes an ADS management scheme for clustered IoT.First,we model the data transmission and anomaly detection in clustered IoT.Then,the operation strategy of the clustered IoT is formulated as the running probabilities of all ADSs deployed on every IoT device.In the presence of a high false positive rate in ADSs,to deal with the trade-off between the security and availability of data,we develop a linear programming model referred to as a security trade-off(ST)model.Next,we develop an analysis framework for the ST model,and solve the ST model on an IoT simulation platform.Last,we reveal the effect of some factors on the maximum combined detection rate through theoretical analysis.Simulations show that the ADS management scheme can mitigate the data unavailability loss caused by the high false positive rates in ADS.

HIF-2α和Twist2在乳腺癌中的表达及其与预后的关系

目的探讨缺氧诱导因子-2α(HIF-2α)和Twist2在乳腺癌中的表达及其与预后的关系。方法采用免疫组织化学SP法分别检测乳腺癌、纤维腺瘤及癌旁正常组织中HIF-2α、Twist2的表达,并对92例乳腺癌患者的生存结局进行随访。结果乳腺癌组HIF-2α、Twist2的表达高于癌旁正常组织组及纤维腺瘤组(P<0.05)。不同肿瘤大小、有无淋巴结转移乳腺癌患者的HIF-2α阳性率比较有差异(P<0.05),不同组织学分级、有无淋巴结转移及雌激素受体乳腺癌患者的Twist2阳性率比较有差异(P<0.05),乳腺癌中HIF-2α与Twist2的表达呈正相关(P<0.05)。HIF-2α和Twist2阳性乳腺癌患者术后5年生存率均低于阴性患者(P<0.05)。结论 HIF-2α和Twist2在乳腺癌中高表达,两者共同促进肿瘤的浸润、转移,HIF-2α高表达提示患者预后不良,可作为预测乳腺癌患者预后的影响因素之一。

Nanosheet-structured K–Co–MoS_2 catalyst for the higher alcohol synthesis from syngas: Synthesis and activation

The nanosheets structured K–Co–MoS_2 catalyst was prepared through a one-step hydrothermal synthesis combined with the wetness impregnation. The fresh catalyst has a high dispersion of Co–Mo–S active phase and no Co_9S_8 is found. The pure H_2 activated catalyst shows a higher intrinsic activity, especially the C_(2+) OH selectivity for the higher alcohol synthesis compared to the one activated by 5% H_2/N_2 atmosphere. The reason is attributed to that the pure H_2 activation more effectively suppresses the formation of Co_9S_8 and stabilizes the Co–Mo–S active phase during the reaction due to the formation of SH species.

Photonic synapses with ultralow energy consumption for artificial visual perception and brain storage

The human visual system,dependent on retinal cells,can be regarded as a complex combination of optical system and nervous system.Artificial retinal system could mimic the sensing and processing function of human eyes.Optically stimulated synaptic devices could serve as the building blocks for artificial retinas and subsequent information transmission system to brain.Herein,photonic synaptic transistors based on polycrystalline MoS_(2),which could simulate human visual perception and brain storage,are presented.Moreover,the photodetection range from visible light to near-infrared light of MoS_(2) multilayer could extend human eyes’vision limitation to near-infrared light.Additionally,the photonic synaptic transistor shows an ultrafast speed within 5μs and ultralow power consumption under optical stimuli about 40 aJ,several orders of magnitude lower than biological synapses(50 ms and 10 fJ).Furthermore,the backgate control could act as emotional modulation of the artificial brain to enhance or suppress memory function,i.e.the intensity of photoresponse.The proposed carrier trapping/detrapping as the main working mechanism is presented for the device.In addition,synaptic functionalities including short synaptic plasticity,long synaptic plasticity and paired-pulse facilitation could be successfully simulated based on the prepared device.Furthermore,the large difference between short synaptic plasticity and long synaptic plasticity reveals the better image pre-processing function of the prepared photonic synapses.The classical Pavlovian conditioning associated with the associative learning is successfully implemented as well.Therefore,the efficient and rich functionalities demonstrate the potential of the MoS_(2) synaptic device that integrates sensing-memory-preprocessing capabilities for realizing artificial neural networks with different emotions that mimic human retina and brain.

Changes in the population structure of Calanus sinicus during summer–autumn in the southern Yellow Sea

Calanus sinicus is a calanoid copepod widely distributed in coastal waters of China and Japan, and oversummering strategies may have major impacts on their population dynamics which in turn affect local marine food web structure. The abundance, stage composition, and sex composition of the planktonic copepod C. sinicus were studied from August to October 2002 in the southern Yellow Sea to understand how its population recovers from the over-summering state. Results showed that C. sinicus had low reproduction in August due to high temperature, except in waters near the Cheju Island with rich food and moderate bottom temperature, but the reproduction rates here decreased in September–October as food availability declined. When temperature dropped in September–October, C. sinicus actively propagated in coastal shallow waters. However, reproduction rates of C. sinicus individuals inhabiting the Yellow Sea Cold Water Mass(YSCWM) remained low during the three months of the study. The percentage of C. sinicus females was high during the reproductive period, which suggests that the sex composition of adult C. sinicus may reflect whether or not the population is in the reproductive mode.Numerous fifth copepodite stage(CV) C. sinicus aggregated in the YSCWM in a suspended developmental stage during the three months of this study, and they potentially served as the parental individuals for population development when conditions became optimal for reproduction later in the year.

Visible-light deposition of CrO_(x) cocatalyst on TiO_(2):Cr valence regulation for superior photocatalytic CO_(2)reduction to CH_(4)

Photodeposition is widely adopted for implanting metal/metal oxide cocatalysts on semiconductors.However,it is prerequisite that the photon energy should be sufficient to excite the host semiconductor.Here,we report a lower-energy irradiation powered deposition strategy for implanting CrO_(x) cocatalyst on TiO_(2).Excitingly,CrO_(x)-400 implanted under visible-light irradiation significantly promotes the CH4 evolution rate on TiO_(2)to 8.4μmolg·^(-1)h^(-1) with selectivity of98%from photocatalytic CO_(2)reduction,which is 15 times of that on CrO_(x)-200 implanted under UV-visible-light irradiation.Moreover,CrO_(x)-400 is identified to be composed of higher valence Cr species compared to CrO_(x)-200.This valence states regulation of Cr species is indicated to provide more active sites for CO_(2) adsorption/activation and to modulate the reaction mechanism from single Cr site to Cr-Cr dual sites,thus endowing the superior CH_(4)production.This work demonstrates an alternative strategy for constructing efficient metal oxides cocatalysts on wide bandgap semiconductor.

Physical processes determining the distribution patterns of Nemopilema nomurai in the East China Sea

The giant jellyfish Nemopilema nomurai is the largest and most dangerous jellyfish species in East Asian waters,and the N.nomurai bloom causes serious problem in coastal industries,fisheries,and tourism.In the previous surveys,we found N.nomurai could not be observed in the south of 30°N.In this paper,we analyzed the mechanism of this phenomenon.After exploring the possible impacts of different environmental factors,we found that physical processes are essential to the distribution pattern of N.nomurai rather than biological or chemical factors in the East China Sea.The combination of the location of the initial breeding places of N.nomurai and the current system determine the distribution pattern.This study could provide important insights to the potential control of the giant jellyfish in the Chinese coastal waters.

护士分层级管理对心内科护士护理质量的影响

目的分析在心内科护士中开展护士分层级管理的效果。方法选择2020年4月—2022年4月山东颐养健康集团新汶中心医院心内科的12名在职护士展开研究,通过对全部护士开展护士分层级管理措施,提高护理质量水平为主要目标。比较管理前后的护理质量、职业素养水平、护理风险发生情况。结果管理后,护士基础护理、护理文书、病房护理、安全意识、消毒隔离各项护理质量评分均较管理前明显更高,差异有统计学意义(P<0.05);管理后,护士专业知识评分和技术操作评分均较管理前明显更高,差异有统计学意义(P<0.05);管理后,护士护理差错事件发生率、不良反应发生率均较管理前明显降低,差异有统计学意义(P<0.05)。结论护士分层级管理应用于心内科护士管理中可以显著提高护理质量水平,有效增强护士的职业素养水平,并且可显著降低护理风险事件地发生。

Two Ultraviolet Radiation Datasets that Cover China

Ultraviolet（UV） radiation has significant effects on ecosystems, environments, and human health, as well as atmospheric processes and climate change. Two ultraviolet radiation datasets are described in this paper. One contains hourly observations of UV radiation measured at 40 Chinese Ecosystem Research Network stations from 2005 to 2015. CUV3 broadband radiometers were used to observe the UV radiation, with an accuracy of 5%, which meets the World Meteorology Organization＇s measurement standards. The extremum method was used to control the quality of the measured datasets. The other dataset contains daily cumulative UV radiation estimates that were calculated using an all-sky estimation model combined with a hybrid model. The reconstructed daily UV radiation data span from 1961 to 2014. The mean absolute bias error and root-mean-square error are smaller than 30% at most stations, and most of the mean bias error values are negative, which indicates underestimation of the UV radiation intensity. These datasets can improve our basic knowledge of the spatial and temporal variations in UV radiation. Additionally, these datasets can be used in studies of potential ozone formation and atmospheric oxidation, as well as simulations of ecological processes.

Iridocorneal Endothelial Syndrome in a Chinese Child

Purpose: We reported a rare case of ICE syndrome in a Chinese child.Case report: A 6-year-old child was admitted to the ophthalmology department of the Affiliated Wuxi Second Hospital of Nanjing Medical University, complaining of elevated intraocular pressure at his first diagnosis in a local hospital. The patient was initially treated with medication..During subsequent follow up,.two iris holes were seen in the patient's right eye and he was then diagnosed with ICE syndrome. The child underwent surgery combined with mitomycin C intraoperatively.The intraocular pressure was stably controlled.Conclusion:.ICE syndrome has been rarely reported in children. Glaucoma filtering surgery combined with intraoperative administration of mitomycin is efficacious for intraocular pressure control.

A Review of the Engineering Role of Burrowing Animals: Implication of Chinese Pangolin as an Ecosystem Engineer

Ecosystem engineers are organisms that alter the distribution of resources in the environment by creating,modifying,maintaining and/or destroying the habitat.They can affect the structure and function of the whole ecosystem furthermore.Burrowing engineers are an important group in ecosystem engineers as they play a critical role in soil translocation and habitat creation in various types of environment.However,few researchers have systematically summarized and analyzed the studies of burrowing engineers.We reviewing the existing ecological studies of burrowing engineer about their interaction with habitat through five directions:(1)soil turnover;(2)changing soil physicochemical properties;(3)changing plant community structure;(4)providing limited resources for commensal animals;and/or(5)affecting animal communities.The Chinese pangolin(Manis pentadactyla)is a typical example of burrowing mammals,in part(5),we focus on the interspecific relationships among burrow commensal species of Chinese pangolin.The engineering effects vary with environmental gradient,literature indicates that burrowing engineer play a stronger role in habitat transformation in the tropical and subtropical areas.The most common experiment method is comparative measurements(include different spatial and temporal scale),manipulative experiment is relatively few.We found that most of the engineering effects had positive feedback to the local ecosystem,increased plant abundance and resilience,increased biodiversity and consequently improved ecosystem functioning.With the global background of dramatic climate change and biodiversity loss in recent decades,we recommend future studies should improving knowledge of long-term engineering effects on population scale and landscape scale,exploring ecological cascades through trophic and engineering pathways,to better understand the attribute of the burrowing behavior of engineers to restore ecosystems and habitat creation.The review is presented as an aid to systematically expound the engineering effect of burrowing animals in the ecosystem,and provided new ideas and advice for planning and implementing conservation management.

Feeding strategies of Euphausia superba in the eastern South Shetland Islands in austral summer

Euphausia superba is a key species in the Southern Ocean that serves as a link between primary production and higher trophic levels.To investigate the feeding strategies of E.superba from the eastern South Shetland Islands,fatty acid biomarkers,stable isotope signatures,and an incubation experiment were conducted.The results of the incubation experiment proved that adult E.superba mainly fed on 2–20μm particles,demonstrating the importance of nanoplankton in their diet.Moreover,significant positive relationships betweenδ15N and body size demonstrated that size-related dietary shifts were present in E.superba.Evidence from principal component analysis and the C16:1ω7/C18:4ω3 ratio showed that juveniles preferentially fed on dinoflagellates and adults were more likely to feed on diatoms.Fatty acid profiles in adult E.superba roughly mirrored the different trophic conditions and feeding strategies between stations.Adult E.superba at Stas D2-07,D5-07,DA-01 and DA-02 exhibited elevated levels of C16:1ω7,C18:4ω3,C18:1ω9 and C18:1ω9/C18:1ω7,indicating higher levels of feeding on both phytoplankton and higher trophic diets.In contrast,adult E.superba at Stas D1-03 and D1-04 were characterized by high levels of polyunsaturated fatty acids/saturated fatty acids ratios and low levels of C16:1ω7,C18:1ω7,C18:4ω3,C18:1ω9 and total fatty acids.We inferred that adult krill at Stas D1-03 and D1-04 still suffered from difficult dietary conditions after overwintering.The different dietary conditions between stations suggest a highly plastic feeding strategy of E.superba in the eastern South Shetland Islands.

Effect of Mn Promoter on Structure and Performance of K-Co-Mo Catalyst for Synthesis of Higher Alcohols from CO Hydrogenation

A series of Mn-doped K-Co-Mo catalysts were prepared by a sol-gel method. The catalyst structure was well characterized by X-ray diffraction, N2 physisorption, NH3 temperature- programmed adsorption, in situ diffuse reflectance infrared Fourier transform spectroscopy, and X-ray absorption fine structure spectroscopy. The catalytic performance for higher alcohol synthesis from syngas was measured. It was found that the Mn-doped catalysts ex- hibited a much higher activity as compared to the unpromoted catalyst, and in particular the C2＋ alcohol selectivity increased significantly. The distribution of alcohol products de- viated from the Anderson-Schulz-Flory law. The portion of methanol in total alcohol was suppressed remarkably and the ethanol became the predominant product. Characterization results indicated that the incorporation of Mn enhanced the interaction of Co and Mo and thus led to the formation of Co-Mo-O species, which was regarded as the active site for the alcohol synthesis. Secondly, the presence of Mn reduced the amount of strong acid sites significantly and meanwhile promoted the formation of weak acid sites, which had a positive effect on the synthesis of alcohol. Furthermore, it was found that the incorporation of Mn can enhance the adsorption of linear- and bridge-type CO significantly, which contributed to the formation of alcohol and growth of carbon chain and thus increased the selectivity to C2＋OH.