Objective: To observe the effect of MTA1 gene silencing by RNA interference on invasion and migration of esophageal carcinoma 9706 cells. Methods: siRNA expression vector targeting MTA1 gene was transfected into EC970...Objective: To observe the effect of MTA1 gene silencing by RNA interference on invasion and migration of esophageal carcinoma 9706 cells. Methods: siRNA expression vector targeting MTA1 gene was transfected into EC9706 cells by Lipofectamine method. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western Blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitro. Results: MTA1 gene expression decreased significantly. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and EC9706 cells transfected with blank vector (P < 0.05). Conclusion: MTA1 gene silencing by RNAi can inhibit the invasion and migration of esophageal carcinoma effectively. It is supposed that MTA1 gene may be a prospective molecule target in tumor therapy.展开更多
基金Supported by a grant from the "Tenth Five-Year Plan" Research Foundation for the Key Construction Project (211 Projects) by Ministry of Education of China (2002)
文摘Objective: To observe the effect of MTA1 gene silencing by RNA interference on invasion and migration of esophageal carcinoma 9706 cells. Methods: siRNA expression vector targeting MTA1 gene was transfected into EC9706 cells by Lipofectamine method. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western Blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitro. Results: MTA1 gene expression decreased significantly. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and EC9706 cells transfected with blank vector (P < 0.05). Conclusion: MTA1 gene silencing by RNAi can inhibit the invasion and migration of esophageal carcinoma effectively. It is supposed that MTA1 gene may be a prospective molecule target in tumor therapy.
