背景与目的肺癌是世界上最常见的恶性肿瘤,其中小细胞肺癌是恶性程度最高的亚型,具有生长迅速、早期转移和高度血管化等特点。阿帕替尼(Apatinib)是我国自主研发的血管内皮生长因子受体2抑制剂,在多种实体瘤中疗效显著。本研究旨在探讨A...背景与目的肺癌是世界上最常见的恶性肿瘤,其中小细胞肺癌是恶性程度最高的亚型,具有生长迅速、早期转移和高度血管化等特点。阿帕替尼(Apatinib)是我国自主研发的血管内皮生长因子受体2抑制剂,在多种实体瘤中疗效显著。本研究旨在探讨Apatinib对小细胞肺癌细胞株NCI-H446的体外作用以及联合哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)抑制剂CCI-779对小细胞肺癌的体外作用。方法体外培养小细胞肺癌细胞株NCI-H446,CCK8法、细胞凋亡实验、细胞周期实验及Transwell实验检测Apatinib及联合mTOR抑制剂CCI-779对NCI-H446细胞增殖、凋亡、周期及迁移的影响;Western blot实验检测血管内皮生长因子受体和细胞周期相关蛋白的表达。结果CCK8实验结果显示高浓度Apatinib能抑制NCI-H446细胞增殖;细胞凋亡实验结果显示高浓度Apatinib诱导NCI-H446细胞凋亡;Transwell实验结果显示高浓度Apatinib抑制NCI-H446细胞迁移;联合mTOR抑制剂CCI-779后,低浓度Apatinib便能抑制NCI-H446细胞增殖和迁移,诱导细胞凋亡。结论Apatinib对小细胞肺癌细胞株NCI-H446的作用具有浓度依赖性特征,高浓度Apatinib能够抑制NCI-H446细胞增殖和迁移,诱导细胞凋亡,与mTOR抑制剂CCI-779联用能增加NCI-H446细胞对Apatinib的敏感性。展开更多
The wave velocity analysis of rock medium is the main method used to explore the internal compositions in the crust and research seismic.In this paper,a compression–shear coupled nonlinear elastic constitutive relati...The wave velocity analysis of rock medium is the main method used to explore the internal compositions in the crust and research seismic.In this paper,a compression–shear coupled nonlinear elastic constitutive relation is established,which is consistent with the mechanical properties of rock and mineral medium under high pressure.On this basis,numerical solutions of the wave equation and plane wave analytical solutions for the primary and secondary wave velocities are obtained.As is indicated by the comparison with the linear elastic constitutive theory,the results reflect the compression–shear coupling characteristics of the rock,including the stress path effect and the compression–shear coupling wave effect.With different parameter values,the velocity of the secondary wave changes from lower than that of the elastic shear wave,to higher than that of the elastic shear wave.The research results are expected to provide meaningful explanations for the physical mechanisms of the supershear wave and sub-Rayleigh wave,and guidance for the detection of rock and soil composition and the observation of seismic waves.展开更多
The objective of this study is to investigate numerically the flow characteristics of falling film on horizontal circular tubes. Numerical simulations are performed using FLUENT for 2D configurations with one and two ...The objective of this study is to investigate numerically the flow characteristics of falling film on horizontal circular tubes. Numerical simulations are performed using FLUENT for 2D configurations with one and two cylinders. The volume of fluid method is used to track the motion of liquid falling film and the gas-liquid interface. The effect of flow characteristics on heat and transfer coefficient may be remarkable, although it has been neglected in previous studies. The velocity distribution and the film thickness characteristics on the top tube, some special flow characteristics on the bottom tube, intertube flow modes and effect of liquid feeder height on flow characteristics have been studied. Our simulations indicate that 1) the velocity distributions of the upper and lower parts of the tube are not strictly symmetric and non- uniform, 2) the film thickness depends on flow rate and angular distributions, 3) the flow characteristics of the top tube are different from those of the bottom tube, 4) three principal and two intermediate transition modes are distinguished, and 5) the liquid feed height plays an important role on the formation of falling film. The numerical results are in a good agreement with the theoretical values by the Nusselt model and the reported results.展开更多
Knoevenagel condensation of aromatic aldehydes with active methylene compounds such as malononitrile,ethylcyanoacetate,benzimidazol-2-acetonitrile and benzothiazole-2-acetonitrile proceeded very smoothly,catalyzed by ...Knoevenagel condensation of aromatic aldehydes with active methylene compounds such as malononitrile,ethylcyanoacetate,benzimidazol-2-acetonitrile and benzothiazole-2-acetonitrile proceeded very smoothly,catalyzed by nano-Fe_(3)O_(4) encapsulated-silica particles supported primary amine.Both reaction time and yield are satisfying.The advantages of this catalyst are ease of preparation,non-toxicity,low cost,ease of handling and recyclability.展开更多
文摘背景与目的肺癌是世界上最常见的恶性肿瘤,其中小细胞肺癌是恶性程度最高的亚型,具有生长迅速、早期转移和高度血管化等特点。阿帕替尼(Apatinib)是我国自主研发的血管内皮生长因子受体2抑制剂,在多种实体瘤中疗效显著。本研究旨在探讨Apatinib对小细胞肺癌细胞株NCI-H446的体外作用以及联合哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)抑制剂CCI-779对小细胞肺癌的体外作用。方法体外培养小细胞肺癌细胞株NCI-H446,CCK8法、细胞凋亡实验、细胞周期实验及Transwell实验检测Apatinib及联合mTOR抑制剂CCI-779对NCI-H446细胞增殖、凋亡、周期及迁移的影响;Western blot实验检测血管内皮生长因子受体和细胞周期相关蛋白的表达。结果CCK8实验结果显示高浓度Apatinib能抑制NCI-H446细胞增殖;细胞凋亡实验结果显示高浓度Apatinib诱导NCI-H446细胞凋亡;Transwell实验结果显示高浓度Apatinib抑制NCI-H446细胞迁移;联合mTOR抑制剂CCI-779后,低浓度Apatinib便能抑制NCI-H446细胞增殖和迁移,诱导细胞凋亡。结论Apatinib对小细胞肺癌细胞株NCI-H446的作用具有浓度依赖性特征,高浓度Apatinib能够抑制NCI-H446细胞增殖和迁移,诱导细胞凋亡,与mTOR抑制剂CCI-779联用能增加NCI-H446细胞对Apatinib的敏感性。
基金National Natural Science Foundation of China,Grant/Award Numbers:11672286,11872361Opening Foundation of the United Laboratory of High-Pressure Physics and Earthquake Science,Grant/Award Number:2019HPPES01。
文摘The wave velocity analysis of rock medium is the main method used to explore the internal compositions in the crust and research seismic.In this paper,a compression–shear coupled nonlinear elastic constitutive relation is established,which is consistent with the mechanical properties of rock and mineral medium under high pressure.On this basis,numerical solutions of the wave equation and plane wave analytical solutions for the primary and secondary wave velocities are obtained.As is indicated by the comparison with the linear elastic constitutive theory,the results reflect the compression–shear coupling characteristics of the rock,including the stress path effect and the compression–shear coupling wave effect.With different parameter values,the velocity of the secondary wave changes from lower than that of the elastic shear wave,to higher than that of the elastic shear wave.The research results are expected to provide meaningful explanations for the physical mechanisms of the supershear wave and sub-Rayleigh wave,and guidance for the detection of rock and soil composition and the observation of seismic waves.
基金Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 20976118).
文摘The objective of this study is to investigate numerically the flow characteristics of falling film on horizontal circular tubes. Numerical simulations are performed using FLUENT for 2D configurations with one and two cylinders. The volume of fluid method is used to track the motion of liquid falling film and the gas-liquid interface. The effect of flow characteristics on heat and transfer coefficient may be remarkable, although it has been neglected in previous studies. The velocity distribution and the film thickness characteristics on the top tube, some special flow characteristics on the bottom tube, intertube flow modes and effect of liquid feeder height on flow characteristics have been studied. Our simulations indicate that 1) the velocity distributions of the upper and lower parts of the tube are not strictly symmetric and non- uniform, 2) the film thickness depends on flow rate and angular distributions, 3) the flow characteristics of the top tube are different from those of the bottom tube, 4) three principal and two intermediate transition modes are distinguished, and 5) the liquid feed height plays an important role on the formation of falling film. The numerical results are in a good agreement with the theoretical values by the Nusselt model and the reported results.
基金We are grateful to the financial supports for this re-search by the National Natural Science Foundation of China(Grant No.21106090)Postdoctoral Foundation of China(No.2012M511352)Foundation of Low Carbon Fatty Amine Engineering Research Center of Zhejiang Province(No.2012E10033).
文摘Knoevenagel condensation of aromatic aldehydes with active methylene compounds such as malononitrile,ethylcyanoacetate,benzimidazol-2-acetonitrile and benzothiazole-2-acetonitrile proceeded very smoothly,catalyzed by nano-Fe_(3)O_(4) encapsulated-silica particles supported primary amine.Both reaction time and yield are satisfying.The advantages of this catalyst are ease of preparation,non-toxicity,low cost,ease of handling and recyclability.