Morpho-Physiological and Biochemical Characterization of Soybean-Associated Rhizobia and Effect of Their Liquid Inoculant Formulation on Nodulation of Host Plants in the Cameroon Cotton Fields Zone

The aim of this research was to assess the diversity of the Cameroon cotton zone in soybean associated rhizobia in order to formulate the most efficient elite inoculant to boost both the cotton and soybean production. Therefore, soybean associated rhizobia were isolated and characterized morphologically, physiologically and biochemically on YEMA culture media. For each of the two soybean varieties (Houla1 and TGX1910 14F) used, the trials were laid out in two IRAD-fields of North Cameroon (Sanguere-Paul) and Far-North (Soukoundou) respectively, under a complete randomized complete block design, the isolate formulations representing the treatments. The six isolated strains (IS1, IS2, IS3, IS4, IS5, IS6) from which seven liquid inoculant were formulated were revealed to belong to the same slow growing group of rhizobia, with a high level of tolerance to temperature, pH, and salinity, with optimum growth at respectively 28˚C, pH (7 - 9), salt (1% - 5%). Not surprisingly, root nodules were formed by both inoculated and uninoculated soybean plants. However, the most efficient soybean-rhizobia symbiosis for nodulations were isolate IS6 associated to TGX1910 14F variety, and isolate IS5 associated to Houla1variety at Sanguere-Paul. Whereas isolate M was associated to TGX1910 14F variety, Houla 1 variety had affinity with native rhizobia isolates at Soukoundou. The present results suggest the adaptability of rhizobia isolates to a particular soybean variety at a particular cotton fields zone. These findings should be taken into consideration for commercial inoculant formulation.

Relationship between Selected Soil Physico-Chemical Characteristics and Mycorrhizal Status under <i>Triumfetta cordifolia</i>in the Cameroon Western Highlands

There is limited information on the pedological requirements of Triumfetta cordifolia. A starting point for establishing such information requires knowledge on the growing environment of the species. The aim of this study was to assess the physicochemical properties and mycorrhizal status in the rhizosphere of Triumeffa cordifolia. Soil and root samples from the rhizosphere of T. cordifolia were collected from three localities (Santchou, Bandjoun, and Balatchi) in the West Region of Cameroon. The results show that the soils are dominated by a loamy texture and have a mean porosity > 50%. Mean bulk density ranges from 0.91 ± 0.02 to 1.26 ± 0.04 g⋅cm−3. The sum of exchangeable cations ranges from medium (6.45 ± 1.02) to high (11.21 ± 1.35) and are evident of the satisfactory soil organic matter (OM) content in the various localities (5.90% ± 0.42% to 10.65% ± 0.73%). Total nitrogen (TN) content of the soils ranged from low (0.10%) to very high (0.41%). Biological activity is low due to very poor OM quality (mean C/N > 20). The average available phosphorus status ranged from medium (18.32 ± 3.91 ppm) to very high (69.39 ± 26.09 ppm). The Cationic Exchange Capacity (CEC) was moderate (19.28 - 29.28 cmol⋅kg−1) and was mainly contributed by soil organic matter. Base saturation ranged from low (28.0%) to medium (48.83%). Assessment of endomycorrhizal colonization showed that the intensity (I), frequency (Fr) and specific density of spores (Ds) were not significantly different among sites. A high level of available P in the Santchou soils appears to be the major cause for the lowest values of Fr, I, and Ds observed. These results reaffirm the link between soil physicochemical properties and endomycorrhizal infection in T. cordifolia. Site characteristics and soil OM quality are factors to be considered in promoting the establishment of mycorrhizal symbiosis for profitable and sustainable cultivation of T. cordifolia.