Scientific interest in plant reproduction has a long and venerable history going back to early European naturalists including Carl Linnaeus,Christian Konrad Sprengel,Joseph Kölreuter,Fritz and Hermann Müller...Scientific interest in plant reproduction has a long and venerable history going back to early European naturalists including Carl Linnaeus,Christian Konrad Sprengel,Joseph Kölreuter,Fritz and Hermann Müller,George Henslow and of course,Charles Darwin.Darwin can be considered the founder of modern plant reproductive biology.His three books(Darwin 1862,1876,1877)laid the conceptual foundation for the field,and many of the ideas they contain are still topics of active research(reviewed in Barrett 2010).The turn of the 20th century saw the rediscovery of Mendel’s Laws and breeding experiments on flowering plants played an important role in establishing many key elements of modern genetics.By the 1940s,Fisher had developed the population genetic principles for theoretical analysis of the evolution of plant mating systems(Fisher 1941)。展开更多
Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isola...Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isolated 24 microsatellite markers from P.chungensis using Illumina Mi Seq sequencing.Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet.All loci were polymorphic with the number of alleles per locus ranging from 2 to 4.The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708,respectively.The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.展开更多
It has long been recognized that natural selection during the haploid gametophytic phase of the plant life cycle may have widespread importance for rates of evolution and the maintenance of genetic variation.Recent th...It has long been recognized that natural selection during the haploid gametophytic phase of the plant life cycle may have widespread importance for rates of evolution and the maintenance of genetic variation.Recent theoretical advances have further highlighted the significance of gametophytic selection for diverse evolutionary processes.Genomic approaches offer exciting opportunities to address key questions about the extent and effects of gametophytic selection on plant evolution and adaptation.Here,we review the progress and prospects for integrating functional and evolutionary genomics to test theoretical predictions,and to examine the importance of gametophytic selection on genetic diversity and rates of evolution.There is growing evidence that selection during the gametophyte phase of the plant life cycle has important effects on both gene and genome evolution and is likely to have important pleiotropic effects on the sporophyte.We discuss the opportunities to integrate comparative population genomics,genome-wide association studies,and experimental approaches to further distinguish how differential selection in the two phases of the plant life cycle contributes to genetic diversity and adaptive evolution.展开更多
文摘Scientific interest in plant reproduction has a long and venerable history going back to early European naturalists including Carl Linnaeus,Christian Konrad Sprengel,Joseph Kölreuter,Fritz and Hermann Müller,George Henslow and of course,Charles Darwin.Darwin can be considered the founder of modern plant reproductive biology.His three books(Darwin 1862,1876,1877)laid the conceptual foundation for the field,and many of the ideas they contain are still topics of active research(reviewed in Barrett 2010).The turn of the 20th century saw the rediscovery of Mendel’s Laws and breeding experiments on flowering plants played an important role in establishing many key elements of modern genetics.By the 1940s,Fisher had developed the population genetic principles for theoretical analysis of the evolution of plant mating systems(Fisher 1941)。
基金funded by the National Key Basic Research Program of China(2014CB954100)the Key Research Program of the Chinese Academic of Sciences(KJZD-EW-L07)+1 种基金the National Natural Science Foundation of China(31200289,31570384)the Natural Science Foundation of Yunnan Province(2012FB182)
文摘Primula chungensis is a species with considerable floral and mating-system variation,including distylous(outcrossing),homostylous(selfing) and mixed populations that contain both outcrossing and selfing forms.We isolated 24 microsatellite markers from P.chungensis using Illumina Mi Seq sequencing.Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet.All loci were polymorphic with the number of alleles per locus ranging from 2 to 4.The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708,respectively.The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P.chungensis and will inform models of the evolutionary history of mating systems in the species.
基金supported by Discovery Grants from the Natural Sciences and Engineering Research Council of Canada to S.I.W.and S.C.H.B.a University of Toronto Ecology and Evolutionary Biology postdoctoral fellowship to J.L.R.
文摘It has long been recognized that natural selection during the haploid gametophytic phase of the plant life cycle may have widespread importance for rates of evolution and the maintenance of genetic variation.Recent theoretical advances have further highlighted the significance of gametophytic selection for diverse evolutionary processes.Genomic approaches offer exciting opportunities to address key questions about the extent and effects of gametophytic selection on plant evolution and adaptation.Here,we review the progress and prospects for integrating functional and evolutionary genomics to test theoretical predictions,and to examine the importance of gametophytic selection on genetic diversity and rates of evolution.There is growing evidence that selection during the gametophyte phase of the plant life cycle has important effects on both gene and genome evolution and is likely to have important pleiotropic effects on the sporophyte.We discuss the opportunities to integrate comparative population genomics,genome-wide association studies,and experimental approaches to further distinguish how differential selection in the two phases of the plant life cycle contributes to genetic diversity and adaptive evolution.
