Dendritic cells(DCs)are the master regulators of the immune response and engage in constant cross-talk with other immune cells to initiate an appropriate immune response.The outcome of cross-talk with various immune c...Dendritic cells(DCs)are the master regulators of the immune response and engage in constant cross-talk with other immune cells to initiate an appropriate immune response.The outcome of cross-talk with various immune cells towards DC functions has been shown to be dependent on the particular subset of cells and their activation stimuli.Here we show that for human B cells to induce maturation of DCs with an enhanced T-cell stimulatory capacity,B-cell receptor(BCR)signaling is sufficient for memory B-cells,whereas additional CD40 signaling is required for naïve B cells to induce complete activation of DCs.展开更多
Basophils are rare granulocytes.Despite representing only~0.5%of all leukocytes,basophils have several important physiological functions.1,2 Although basophils lack the classic features of professional antigen-present...Basophils are rare granulocytes.Despite representing only~0.5%of all leukocytes,basophils have several important physiological functions.1,2 Although basophils lack the classic features of professional antigen-presenting cells,3–7 through the secretion of cytokines,they orient the immune response by polarizing Th2 differentiation and supporting B-cell differentiation and class switching.Basophils are also critical for mediating protection against helminth infection.1,2,8,9 Basophils receive activation signals from diverse sources.It is well recognized that cytokines such as IL-3,granulocyte–macrophage colony-stimulating factor(GM-CSF),thymic stromal lymphopoietin(TSLP)and IL-33;various toll-like receptor ligands;allergen-bound IgE provide activation signals to basophils and induce the release of inflammatory mediators.10–15 In addition,several reports have also demonstrated the existence of anti-IgE autoantibodies that possess the capacity to induce basophil activation in patients with chronic spontaneous urticaria(CSU),atopic or non-atopic asthma or autoimmune disease.16–21 However,isolation and functional exploration of such anti-IgE IgG autoantibodies from either healthy donors or patients have not been attempted yet.By using a pooled normal IgG preparation from healthy donors,specifically intravenous immunoglobulin G(IVIG)22 that represents the complete IgG repertoire of a normal individual,we attempted to address this outstanding question in the field.展开更多
基金Supported by the Institut National de la Santé et de la Recherche Médicale (INSERM),Sorbonne Université and Université Paris Descartes, Paris, France.
文摘Dendritic cells(DCs)are the master regulators of the immune response and engage in constant cross-talk with other immune cells to initiate an appropriate immune response.The outcome of cross-talk with various immune cells towards DC functions has been shown to be dependent on the particular subset of cells and their activation stimuli.Here we show that for human B cells to induce maturation of DCs with an enhanced T-cell stimulatory capacity,B-cell receptor(BCR)signaling is sufficient for memory B-cells,whereas additional CD40 signaling is required for naïve B cells to induce complete activation of DCs.
基金supported by the Institut National de la Santéet de la Recherche Médicale(INSERM)Sorbonne Université,UniversitéParis Descartes,ANR-19-CE17-0021(BASIN)CSL Behring,Switzerland.C.G.and A.K.were the recipients of fellowships from La Fondation pour la Recherche Médicale(FDM20150633674 and FDT201805005552,respectively),France。
文摘Basophils are rare granulocytes.Despite representing only~0.5%of all leukocytes,basophils have several important physiological functions.1,2 Although basophils lack the classic features of professional antigen-presenting cells,3–7 through the secretion of cytokines,they orient the immune response by polarizing Th2 differentiation and supporting B-cell differentiation and class switching.Basophils are also critical for mediating protection against helminth infection.1,2,8,9 Basophils receive activation signals from diverse sources.It is well recognized that cytokines such as IL-3,granulocyte–macrophage colony-stimulating factor(GM-CSF),thymic stromal lymphopoietin(TSLP)and IL-33;various toll-like receptor ligands;allergen-bound IgE provide activation signals to basophils and induce the release of inflammatory mediators.10–15 In addition,several reports have also demonstrated the existence of anti-IgE autoantibodies that possess the capacity to induce basophil activation in patients with chronic spontaneous urticaria(CSU),atopic or non-atopic asthma or autoimmune disease.16–21 However,isolation and functional exploration of such anti-IgE IgG autoantibodies from either healthy donors or patients have not been attempted yet.By using a pooled normal IgG preparation from healthy donors,specifically intravenous immunoglobulin G(IVIG)22 that represents the complete IgG repertoire of a normal individual,we attempted to address this outstanding question in the field.
